PMID- 25304355
OWN - NLM
STAT- MEDLINE
DCOM- 20150624
LR  - 20211021
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 1234
DP  - 2015
TI  - Assessing GPCR and G protein signaling to the nucleus in live cells using 
      fluorescent biosensors.
PG  - 149-59
LID - 10.1007/978-1-4939-1755-6_13 [doi]
AB  - G protein-coupled receptor (GPCR) signaling cascades regulate a wide variety of 
      cellular processes and feature prominently in many cardiovascular pathologies. As 
      such they represent major drug targets and discovering novel aspects of GPCR 
      signaling provide important opportunities to identify additional potential 
      therapeutic approaches to reverse or prevent cardiac remodeling and failure. 
      Monitoring cellular trafficking of signaling components and specific protein 
      kinase activities using fluorescent biosensors has provided key insight into 
      stress/GPCR-induced kinase signaling networks and their effect on cardiac gene 
      expression. Herein we describe the protocols for the expression, visualization 
      (by confocal microscopy), and interpretation of data obtained with such 
      biosensors expressed in adult cardiomyocytes. Our focus is on the cellular 
      trafficking of class II histone deacetylases (i.e., HDAC5) and on the FRET sensor 
      (Camui) for calmodulin-dependent protein kinase II (CaMKII).
FAU - Bossuyt, Julie
AU  - Bossuyt J
AD  - Department of Pharmacology, University of California, Davis, CA, 95616-8636, USA.
FAU - Bers, Donald M
AU  - Bers DM
LA  - eng
GR  - R01 HL103933/HL/NHLBI NIH HHS/United States
GR  - R37 HL30077/HL/NHLBI NIH HHS/United States
GR  - P01 HL080101/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (Receptors, G-Protein-Coupled)
RN  - EC 3.5.1.98 (Histone Deacetylases)
RN  - EC 3.6.1.- (GTP-Binding Proteins)
SB  - IM
MH  - Animals
MH  - *Biosensing Techniques
MH  - Cell Culture Techniques
MH  - Cell Nucleus/*metabolism
MH  - *Fluorescence Resonance Energy Transfer
MH  - GTP-Binding Proteins/*metabolism
MH  - Histone Deacetylases/metabolism
MH  - Mice
MH  - Microscopy, Confocal
MH  - Molecular Imaging/methods
MH  - Myocytes, Cardiac/metabolism
MH  - Receptors, G-Protein-Coupled/*metabolism
EDAT- 2014/10/12 06:00
MHDA- 2015/06/25 06:00
CRDT- 2014/10/12 06:00
PHST- 2014/10/12 06:00 [entrez]
PHST- 2014/10/12 06:00 [pubmed]
PHST- 2015/06/25 06:00 [medline]
AID - 10.1007/978-1-4939-1755-6_13 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2015;1234:149-59. doi: 10.1007/978-1-4939-1755-6_13.