PMID- 25304970
OWN - NLM
STAT- MEDLINE
DCOM- 20160302
LR  - 20171116
IS  - 1477-0903 (Electronic)
IS  - 0960-3271 (Linking)
VI  - 34
IP  - 6
DP  - 2015 Jun
TI  - Expression of selected proteins of the extrinsic and intrinsic pathways of 
      apoptosis in human leukocytes exposed to N-nitrosodimethylamine.
PG  - 591-600
LID - 10.1177/0960327114551391 [doi]
AB  - N-nitrosodimethylamine (NDMA) is a xenobiotic widespread in human environment 
      capable of regulating the lifespan of immune cells. In this study, we examined 
      the roles of the tumor necrosis factor-related apoptosis-inducing ligand 
      (TRAIL)/death receptor 5 (DR5) complex and the Fas molecule in the induction of 
      the extrinsic apoptosis pathway in human neutrophils (polymorphonuclear 
      neutrophils (PMNs)) and peripheral blood mononuclear cells (PBMCs) exposed to 
      NDMA. Also we assessed these proteins ability to trigger the intrinsic apoptosis 
      pathway in those cells. For this purpose, we examined the expression of 
      Fas-associated protein with death domain, truncated Bid (tBid) proteins, and 
      apoptogenic factors such as apoptosis-inducing factor, Smac/Diablo, Omi/HtrA2, 
      and caspase-3 as an indication of accomplished apoptosis phenomenon. PMNs and 
      PBMCs were isolated from whole blood by density gradient centrifugation using 
      Polymorphrep. Apoptotic cells were assessed with flow cytometry using a 
      ready-made kit. The expression of proapoptotic molecules was investigated by 
      Western blot analysis of PMNs and PBMCs treated with NDMA and/or rhTRAIL. The 
      obtained results confirm the proapoptotic effects of NDMA on the examined human 
      leukocytes and indicate an active participation of the TRAIL/DR5 complex and Fas 
      protein in the process of apoptosis. Moreover, the research revealed distinct 
      mechanisms of intrinsic apoptosis pathway activation between PMNs and PBMCs 
      exposed to NDMA, as confirmed by the different levels of tBid, Smac/Diablo, 
      Omi/HtrA2, and caspase-3 expression in those cells.
CI  - (c) The Author(s) 2014.
FAU - Iwaniuk, A
AU  - Iwaniuk A
AD  - Department of Immunology, Medical University of Bialystok, Poland 
      agnieszka.iwaniuk@umb.edu.pl.
FAU - Jablonska, E
AU  - Jablonska E
AD  - Department of Immunology, Medical University of Bialystok, Poland.
FAU - Jablonski, J
AU  - Jablonski J
AD  - Department of Toxicology, Medical University of Bialystok, Poland.
FAU - Ratajczak-Wrona, W
AU  - Ratajczak-Wrona W
AD  - Department of Immunology, Medical University of Bialystok, Poland.
FAU - Garley, M
AU  - Garley M
AD  - Department of Immunology, Medical University of Bialystok, Poland.
LA  - eng
PT  - Journal Article
DEP - 20141010
PL  - England
TA  - Hum Exp Toxicol
JT  - Human & experimental toxicology
JID - 9004560
RN  - 0 (FAS protein, human)
RN  - 0 (Receptors, TNF-Related Apoptosis-Inducing Ligand)
RN  - 0 (TNF-Related Apoptosis-Inducing Ligand)
RN  - 0 (fas Receptor)
RN  - M43H21IO8R (Dimethylnitrosamine)
SB  - IM
MH  - Adult
MH  - Apoptosis/physiology
MH  - Cells, Cultured
MH  - Dimethylnitrosamine/*toxicity
MH  - Female
MH  - Humans
MH  - Leukocytes, Mononuclear/*drug effects/metabolism
MH  - Male
MH  - Neutrophils/*drug effects/metabolism
MH  - Receptors, TNF-Related Apoptosis-Inducing Ligand/metabolism
MH  - TNF-Related Apoptosis-Inducing Ligand/metabolism
MH  - Young Adult
MH  - fas Receptor/metabolism
OTO - NOTNLM
OT  - Apoptosis/cell death
OT  - N-nitrosodimethylamine
OT  - neutrophils
OT  - peripheral blood mononuclear cells
EDAT- 2014/10/12 06:00
MHDA- 2016/03/05 06:00
CRDT- 2014/10/12 06:00
PHST- 2014/10/12 06:00 [entrez]
PHST- 2014/10/12 06:00 [pubmed]
PHST- 2016/03/05 06:00 [medline]
AID - 0960327114551391 [pii]
AID - 10.1177/0960327114551391 [doi]
PST - ppublish
SO  - Hum Exp Toxicol. 2015 Jun;34(6):591-600. doi: 10.1177/0960327114551391. Epub 2014 
      Oct 10.