PMID- 25308193
OWN - NLM
STAT- MEDLINE
DCOM- 20150804
LR  - 20171116
IS  - 1879-3177 (Electronic)
IS  - 0887-2333 (Linking)
VI  - 29
IP  - 1
DP  - 2015 Feb
TI  - Comparative study on thiol drugs' effect on tert-butyl hydroperoxide induced 
      luminol chemiluminescence in human erythrocyte lysate and hemoglobin oxidation.
PG  - 148-54
LID - S0887-2333(14)00165-9 [pii]
LID - 10.1016/j.tiv.2014.09.001 [doi]
AB  - The current studies have investigated the effect of heterocyclic drugs with the 
      single thiol group (thiamazole, mercaptopurine) and dithiol aliphatic drugs 
      (dimercaptosuccinic acid, dithiothreitol) under oxidative stress conditions, 
      using tert-butyl hydroperoxide (t-BuOOH), in human erythrocyte lysate with the 
      luminol-enhanced chemiluminescence technique. Knowing that oxidative processes 
      induced by t-BuOOH are triggered by (oxy)hemoglobin (Hb), the effect of different 
      thiol drugs (RSH) on isolated human Hb oxidation to methemoglobin (MHb) and 
      hemichromes (HChr) was further considered. Three types of chemiluminescence 
      curves, fitting to logistic-exponential model, have been revealed under influence 
      of RSH. Structure of the data (MHb and HChr production, and free radical activity 
      of RSH) in Principal Component Analysis visualization and kinetic profiles of 
      chemiluminescence integrate information in terms of the diversity of RSH reaction 
      mechanisms depending on the specific molecular context of the given thiol: 
      aliphatic or aromatic nature as well as the number and position of the -SH groups 
      in the molecule. The study conducted in presented in vitro systems indicates the 
      potential role of thiol drugs mediated toxicity in an oxidative stress dependent 
      mechanism.
CI  - Copyright (c) 2014 Elsevier Ltd. All rights reserved.
FAU - Sajewicz, Waldemar
AU  - Sajewicz W
AD  - Department of Biomedical and Environmental Analyses, Wroclaw Medical University, 
      Borowska St. 211, PL 50-556 Wroclaw, Poland. Electronic address: 
      waldemar.sajewicz@umed.wroc.pl.
FAU - Zalewska, Marta
AU  - Zalewska M
AD  - Department of Biomedical and Environmental Analyses, Wroclaw Medical University, 
      Borowska St. 211, PL 50-556 Wroclaw, Poland.
FAU - Milnerowicz, Halina
AU  - Milnerowicz H
AD  - Department of Biomedical and Environmental Analyses, Wroclaw Medical University, 
      Borowska St. 211, PL 50-556 Wroclaw, Poland.
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20141013
PL  - England
TA  - Toxicol In Vitro
JT  - Toxicology in vitro : an international journal published in association with 
      BIBRA
JID - 8712158
RN  - 0 (Hemoglobins)
RN  - 554Z48XN5E (Methimazole)
RN  - 5EXP385Q4F (Luminol)
RN  - 955VYL842B (tert-Butylhydroperoxide)
RN  - DX1U2629QE (Succimer)
RN  - E7WED276I5 (Mercaptopurine)
RN  - T8ID5YZU6Y (Dithiothreitol)
SB  - IM
MH  - Dithiothreitol/*pharmacology
MH  - Erythrocytes/*drug effects
MH  - Hemoglobins/drug effects/*metabolism
MH  - Humans
MH  - Luminescence
MH  - *Luminescent Measurements/methods
MH  - *Luminol
MH  - Mercaptopurine/*pharmacology
MH  - Methimazole/*pharmacology
MH  - Oxidation-Reduction/drug effects
MH  - Oxidative Stress/drug effects
MH  - Succimer/*pharmacology
MH  - *tert-Butylhydroperoxide
OTO - NOTNLM
OT  - Chemiluminescence
OT  - Erythrocyte lysate
OT  - Hemichromes
OT  - Methemoglobin
OT  - Principal Component Analysis
OT  - Thiol drugs
EDAT- 2014/10/14 06:00
MHDA- 2015/08/05 06:00
CRDT- 2014/10/14 06:00
PHST- 2013/09/03 00:00 [received]
PHST- 2014/08/28 00:00 [revised]
PHST- 2014/09/05 00:00 [accepted]
PHST- 2014/10/14 06:00 [entrez]
PHST- 2014/10/14 06:00 [pubmed]
PHST- 2015/08/05 06:00 [medline]
AID - S0887-2333(14)00165-9 [pii]
AID - 10.1016/j.tiv.2014.09.001 [doi]
PST - ppublish
SO  - Toxicol In Vitro. 2015 Feb;29(1):148-54. doi: 10.1016/j.tiv.2014.09.001. Epub 
      2014 Oct 13.