PMID- 25310083
OWN - NLM
STAT- MEDLINE
DCOM- 20150619
LR  - 20141022
IS  - 1791-3004 (Electronic)
IS  - 1791-2997 (Linking)
VI  - 10
IP  - 6
DP  - 2014 Dec
TI  - N‑acetyl‑L‑cysteine reduces arsenite‑induced cytotoxicity through chelation in 
      U937 monocytes and macrophages.
PG  - 2961-6
LID - 10.3892/mmr.2014.2612 [doi]
AB  - In the present study, in order to clarify the preventive mechanism of 
      N‑acetyl‑L‑cysteine (NAC) on arsenite‑induced apoptosis in U937 cells, which lack 
      functional p53, the cytotoxicity among U937 cells [monocytes and 
      12‑O‑tetradecanoylphorbol‑13‑acetate (TPA)‑treated macrophages] receiving NAC 
      treatment at different times post arsenite treatment was examined. TPA‑treated 
      macrophages were more resistant to arsenite‑induced apoptosis than monocytes, 
      which may be associated with the induction of Bcl‑2 expression. Pretreatment with 
      20 mM NAC prior to arsenite exposure suppressed apoptosis up to 75% in the 
      monocytes and 100% in the macrophages. However, 6‑h NAC pretreatment and 
      subsequent washing out of NAC from the culture medium prior to arsenite treatment 
      did not inhibit the arsenite‑induced apoptosis. Post‑treatment by NAC up to 1 h 
      following arsenite exposure almost completely inhibited the cytotoxic effects of 
      arsenite in U937 monocytes and macrophages. The results of the current study 
      indicate that the preventive mechanism of NAC on arsenite‑induced apoptosis in 
      U937 monocytes and macrophages mainly involves chelation of arsenite in culture 
      medium.
FAU - Ghani, Sidra
AU  - Ghani S
AD  - Department of Epidemiology and Preventive Medicine, Kagoshima University Graduate 
      School of Medical and Dental Sciences, Kagoshima, Kagoshima 890‑8544, Japan.
FAU - Khan, Noureen
AU  - Khan N
AD  - Department of Epidemiology and Preventive Medicine, Kagoshima University Graduate 
      School of Medical and Dental Sciences, Kagoshima, Kagoshima 890‑8544, Japan.
FAU - Koriyama, Chihaya
AU  - Koriyama C
AD  - Department of Epidemiology and Preventive Medicine, Kagoshima University Graduate 
      School of Medical and Dental Sciences, Kagoshima, Kagoshima 890‑8544, Japan.
FAU - Akiba, Suminori
AU  - Akiba S
AD  - Department of Epidemiology and Preventive Medicine, Kagoshima University Graduate 
      School of Medical and Dental Sciences, Kagoshima, Kagoshima 890‑8544, Japan.
FAU - Yamamoto, Megumi
AU  - Yamamoto M
AD  - Integrated Physiology Section, Department of Basic Medical Science, National 
      Institute for Minamata Disease, Minamata, Kumamoto 867‑0008, Japan.
LA  - eng
PT  - Journal Article
DEP - 20141008
PL  - Greece
TA  - Mol Med Rep
JT  - Molecular medicine reports
JID - 101475259
RN  - 0 (Arsenites)
RN  - 0 (Proto-Oncogene Proteins c-bcl-2)
RN  - N5509X556J (arsenite)
RN  - WYQ7N0BPYC (Acetylcysteine)
SB  - IM
MH  - Acetylcysteine/*pharmacology
MH  - Apoptosis/drug effects
MH  - Arsenites/*pharmacology
MH  - Humans
MH  - Macrophages/*drug effects
MH  - Monocytes/*drug effects
MH  - Proto-Oncogene Proteins c-bcl-2
MH  - U937 Cells
EDAT- 2014/10/14 06:00
MHDA- 2015/06/20 06:00
CRDT- 2014/10/14 06:00
PHST- 2013/12/13 00:00 [received]
PHST- 2014/05/14 00:00 [accepted]
PHST- 2014/10/14 06:00 [entrez]
PHST- 2014/10/14 06:00 [pubmed]
PHST- 2015/06/20 06:00 [medline]
AID - 10.3892/mmr.2014.2612 [doi]
PST - ppublish
SO  - Mol Med Rep. 2014 Dec;10(6):2961-6. doi: 10.3892/mmr.2014.2612. Epub 2014 Oct 8.