PMID- 25310568
OWN - NLM
STAT- MEDLINE
DCOM- 20150629
LR  - 20211021
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 9
IP  - 10
DP  - 2014
TI  - A novel model of human skin pressure ulcers in mice.
PG  - e109003
LID - 10.1371/journal.pone.0109003 [doi]
LID - e109003
AB  - INTRODUCTION: Pressure ulcers are a prevalent health problem in today's society. 
      The shortage of suitable animal models limits our understanding and our ability 
      to develop new therapies. This study aims to report on the development of a novel 
      and reproducible human skin pressure ulcer model in mice. MATERIAL AND METHODS: 
      Male non-obese, diabetic, severe combined immunodeficiency mice (n = 22) were 
      engrafted with human skin. A full-thickness skin graft was placed onto 4x3 cm 
      wounds created on the dorsal skin of the mice. Two groups with permanent grafts 
      were studied after 60 days. The control group (n = 6) was focused on the process 
      of engraftment. Evaluations were conducted with photographic assessment, 
      histological analysis and fluorescence in situ hybridization (FISH) techniques. 
      The pressure ulcer group (n = 12) was created using a compression device. A 
      pressure of 150 mmHg for 8 h, with a total of three cycles of compression-release 
      was exerted. Evaluations were conducted with photographic assessment and 
      histological analysis. RESULTS: Skin grafts in the control group took 
      successfully, as shown by visual assessment, FISH techniques and histological 
      analysis. Pressure ulcers in the second group showed full-thickness skin loss 
      with damage and necrosis of all the epidermal and dermal layers (ulcer stage III) 
      in all cases. Complete repair occurred after 40 days. CONCLUSIONS: An 
      inexpensive, reproducible human skin pressure ulcer model has been developed. 
      This novel model will facilitate the development of new clinically relevant 
      therapeutic strategies that can be tested directly on human skin.
FAU - Maldonado, Andres A
AU  - Maldonado AA
AD  - Department of Plastic and Reconstructive Surgery and Burn Unit, University 
      Hospital of Getafe, Madrid, Spain.
FAU - Cristobal, Lara
AU  - Cristobal L
AD  - Department of Plastic and Reconstructive Surgery and Burn Unit, University 
      Hospital of Getafe, Madrid, Spain.
FAU - Martin-Lopez, Javier
AU  - Martin-Lopez J
AD  - Department of Pathology, University Hospital of Puerta de Hierro, Madrid, Spain.
FAU - Mallen, Mar
AU  - Mallen M
AD  - Department of Genetics, University Hospital Central de la Defensa, Madrid, Spain.
FAU - Garcia-Honduvilla, Natalio
AU  - Garcia-Honduvilla N
AD  - Department of Medical Specialties, Faculty of Medicine, University of Alcala, 
      Networking Research Centre on Bioengineering, Biomaterials and Nanomedicine 
      (CIBER-BBN), Madrid, Spain.
FAU - Bujan, Julia
AU  - Bujan J
AD  - Department of Medical Specialties, Faculty of Medicine, University of Alcala, 
      Networking Research Centre on Bioengineering, Biomaterials and Nanomedicine 
      (CIBER-BBN), Madrid, Spain.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20141013
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
SB  - IM
MH  - Animals
MH  - *Disease Models, Animal
MH  - Humans
MH  - Male
MH  - Mice
MH  - Mice, SCID
MH  - Pressure Ulcer/*pathology
MH  - Skin/*pathology
MH  - Skin Transplantation
MH  - Wound Healing
PMC - PMC4195607
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2014/10/14 06:00
MHDA- 2015/06/30 06:00
PMCR- 2014/10/13
CRDT- 2014/10/14 06:00
PHST- 2014/06/05 00:00 [received]
PHST- 2014/09/03 00:00 [accepted]
PHST- 2014/10/14 06:00 [entrez]
PHST- 2014/10/14 06:00 [pubmed]
PHST- 2015/06/30 06:00 [medline]
PHST- 2014/10/13 00:00 [pmc-release]
AID - PONE-D-14-24412 [pii]
AID - 10.1371/journal.pone.0109003 [doi]
PST - epublish
SO  - PLoS One. 2014 Oct 13;9(10):e109003. doi: 10.1371/journal.pone.0109003. 
      eCollection 2014.