PMID- 25311133
OWN - NLM
STAT- MEDLINE
DCOM- 20150611
LR  - 20141014
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 1228
DP  - 2015
TI  - Quantitative analysis of chromosome localization in the nucleus.
PG  - 223-33
LID - 10.1007/978-1-4939-1680-1_17 [doi]
AB  - The spatial organization of the genome within the interphase nucleus is important 
      for mediating genome functions. The radial organization of chromosome territories 
      has been studied traditionally using two-dimensional fluorescence in situ 
      hybridization (FISH) using labeled whole chromosome probes. Information from 
      2D-FISH images is analyzed quantitatively and is depicted in the form of the 
      spatial distribution of chromosomes territories. However, to the best of our 
      knowledge no open-access tools are available to delineate the position of 
      chromosome territories from 2D-FISH images. In this chapter we present a 
      methodology termed Image Analysis of Chromosomes for computing their localization 
      (IMACULAT). IMACULAT is an open-access, automated tool that partitions the cell 
      nucleus into shells of equal area or volume and computes the spatial distribution 
      of chromosome territories.
FAU - Chakraborty, Sandeep
AU  - Chakraborty S
AD  - Department of Biological Sciences, Tata Institute of Fundamental Research, 
      Mumbai, Maharashtra, India, sanchak@gmail.com.
FAU - Mehta, Ishita
AU  - Mehta I
FAU - Kulashreshtha, Mugdha
AU  - Kulashreshtha M
FAU - Rao, B J
AU  - Rao BJ
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
SB  - IM
MH  - Chromosomes/*metabolism
MH  - Fibroblasts/cytology
MH  - Humans
MH  - Image Processing, Computer-Assisted
MH  - In Situ Hybridization, Fluorescence/*methods
EDAT- 2014/10/15 06:00
MHDA- 2015/06/13 06:00
CRDT- 2014/10/15 06:00
PHST- 2014/10/15 06:00 [entrez]
PHST- 2014/10/15 06:00 [pubmed]
PHST- 2015/06/13 06:00 [medline]
AID - 10.1007/978-1-4939-1680-1_17 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2015;1228:223-33. doi: 10.1007/978-1-4939-1680-1_17.