PMID- 25311486
OWN - NLM
STAT- MEDLINE
DCOM- 20150518
LR  - 20181202
IS  - 1873-3778 (Electronic)
IS  - 0021-9673 (Linking)
VI  - 1367
DP  - 2014 Nov 7
TI  - Using pollen grains as novel hydrophilic solid-phase extraction sorbents for the 
      simultaneous determination of 16 plant growth regulators.
PG  - 39-47
LID - S0021-9673(14)01527-1 [pii]
LID - 10.1016/j.chroma.2014.09.071 [doi]
AB  - In this article, pollen grains were for the first time used as a hydrophilic 
      solid-phase extraction (HILIC-SPE) sorbent for the determination of 16 plant 
      growth regulators (PGRs) in fruits and vegetables. Fourier transform infrared 
      spectroscopy (FT-IR), scanning electronic microscopy (SEM) and nitrogen sorption 
      porosimetry (NSP) were used to investigate the chemical structure and the surface 
      properties of the pollen grains. Pollen grains exhibited an excellent adsorption 
      capacity for some polar compounds due to their particular functional groups. 
      Several parameters influencing extraction performance were investigated. A green 
      and simple HILIC-SPE-method using pollen grain cartridge for purification of 
      fruit and vegetable extractions, followed by ultra-high performance liquid 
      chromatography-triple quadrupole tandem mass spectrometry (UHPLC-MS/MS) was 
      established. Good linear relationships were obtained for 16 PGRs with correlation 
      coefficients (R) above 0.9980. The limits of detection (LODs) of 16 PGRs in 
      cucumber were in the range of 0.01-1.10 mug . kg(-1). Reproducibility of the 
      method was evaluated by intra-day and inter-day precisions with relative standard 
      deviations (RSDs), which were less than 14.4%. We successfully applied this 
      methodology to analyze 16 PGRs in 8 different kinds of fruits and vegetables. The 
      recoveries from samples spiked with 16 PGRs were from 80.5% to 119.2%, with 
      relative standard deviations less than 15.0%.
CI  - Copyright (c) 2014 Elsevier B.V. All rights reserved.
FAU - Lu, Qian
AU  - Lu Q
AD  - Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of 
      Education), Department of Chemistry, Wuhan University, Wuhan, Hubei 430072, PR 
      China.
FAU - Wu, Jian-Hong
AU  - Wu JH
AD  - College of Chemical Engineering, Wuhan Textile University, Wuhan, Hubei 430200, 
      PR China.
FAU - Yu, Qiong-Wei
AU  - Yu QW
AD  - Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of 
      Education), Department of Chemistry, Wuhan University, Wuhan, Hubei 430072, PR 
      China.
FAU - Feng, Yu-Qi
AU  - Feng YQ
AD  - Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of 
      Education), Department of Chemistry, Wuhan University, Wuhan, Hubei 430072, PR 
      China. Electronic address: yqfeng@whu.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20141005
PL  - Netherlands
TA  - J Chromatogr A
JT  - Journal of chromatography. A
JID - 9318488
RN  - 0 (Plant Growth Regulators)
SB  - IM
MH  - Chromatography, High Pressure Liquid
MH  - Fruit/chemistry
MH  - Hydrophobic and Hydrophilic Interactions
MH  - Limit of Detection
MH  - Plant Growth Regulators/*analysis
MH  - Pollen/*chemistry
MH  - Reproducibility of Results
MH  - Solid Phase Extraction/*methods
MH  - Spectroscopy, Fourier Transform Infrared
MH  - Tandem Mass Spectrometry/methods
OTO - NOTNLM
OT  - Hydrophilic solid-phase extraction
OT  - Plant growth regulators
OT  - Pollen grains
OT  - UHPLC-ESI-MS/MS
EDAT- 2014/10/15 06:00
MHDA- 2015/05/20 06:00
CRDT- 2014/10/15 06:00
PHST- 2014/08/30 00:00 [received]
PHST- 2014/09/25 00:00 [revised]
PHST- 2014/09/25 00:00 [accepted]
PHST- 2014/10/15 06:00 [entrez]
PHST- 2014/10/15 06:00 [pubmed]
PHST- 2015/05/20 06:00 [medline]
AID - S0021-9673(14)01527-1 [pii]
AID - 10.1016/j.chroma.2014.09.071 [doi]
PST - ppublish
SO  - J Chromatogr A. 2014 Nov 7;1367:39-47. doi: 10.1016/j.chroma.2014.09.071. Epub 
      2014 Oct 5.