PMID- 25312298
OWN - NLM
STAT- MEDLINE
DCOM- 20150727
LR  - 20141127
IS  - 1365-4632 (Electronic)
IS  - 0011-9059 (Linking)
VI  - 53
IP  - 12
DP  - 2014 Dec
TI  - Dual-color, break-apart fluorescence in situ hybridization probe for 
      distinguishing clear cell sarcoma of soft tissue from malignant melanoma.
PG  - 1464-7
LID - 10.1111/j.1365-4632.2011.05009.x [doi]
AB  - BACKGROUND: Clear cell sarcoma (CCS) of soft tissue is a rare soft tissue sarcoma 
      with melanocytic differentiation and shares morphologic, immunohistochemical, 
      ultrastructural, and molecular features with malignant melanoma (MM). Because the 
      prognosis of CCS is much different from MM, it is important to distinguish each 
      other by selective method. CCS is well-recognized as having the t(12;22)(q13;q12) 
      translocation, on the other hand MM is not. Therefore, detecting Ewing sarcoma 
      region 1 (ESWR1) gene rearrangement can serve as a crucial diagnostic 
      determinant. METHODS: Biopsy was taken from a 52-year-old man who reported a 
      3-year history of a gradually enlarging nodule on the sole of his left foot. 
      Routine and special stains for melanocytic markers and fluorescence in situ 
      hybridization (FISH) evaluation using dual color break-apart rearrangement probes 
      specific for ESWR1 was performed for formalin-fixed tissue. RESULTS: Neoplastic 
      cells expressed diffuse but strong positivity for HMB45 and S100 but not for 
      Melan-A. Dual color, break-apart interphase FISH revealed EWS(22q12) gene 
      rearrangements in CCS tumor cells. CONCLUSION: Fluorescence in situ hybridization 
      evaluation using ESWR1 gene probe for CCS sharing clinical and histopathological 
      characteristics with MM is a valuable tool to distinguish each other.
CI  - (c) 2014 The International Society of Dermatology.
FAU - Yoon, Jung Hee
AU  - Yoon JH
AD  - Department of Dermatology, College of Medicine, Korea University, Guro Hospital, 
      Seoul, Korea.
FAU - Baek, Yoo Sang
AU  - Baek YS
FAU - Jeon, Jiehyun
AU  - Jeon J
FAU - Oh, Chil Hwan
AU  - Oh CH
FAU - Song, Hae Jun
AU  - Song HJ
LA  - eng
PT  - Case Reports
PT  - Journal Article
DEP - 20141014
PL  - England
TA  - Int J Dermatol
JT  - International journal of dermatology
JID - 0243704
RN  - 0 (RNA Probes)
RN  - 0 (RNA-Binding Protein EWS)
SB  - IM
MH  - Diagnosis, Differential
MH  - Foot Diseases/*diagnosis/genetics/pathology
MH  - Gene Rearrangement/genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Male
MH  - Melanoma/*diagnosis/genetics
MH  - Middle Aged
MH  - RNA Probes
MH  - RNA-Binding Protein EWS
MH  - Sarcoma, Clear Cell/*diagnosis/genetics/pathology
MH  - Skin Neoplasms/*diagnosis/genetics/pathology
MH  - Soft Tissue Neoplasms/genetics
MH  - Tissue Array Analysis
EDAT- 2014/10/15 06:00
MHDA- 2015/07/28 06:00
CRDT- 2014/10/15 06:00
PHST- 2014/10/15 06:00 [entrez]
PHST- 2014/10/15 06:00 [pubmed]
PHST- 2015/07/28 06:00 [medline]
AID - 10.1111/j.1365-4632.2011.05009.x [doi]
PST - ppublish
SO  - Int J Dermatol. 2014 Dec;53(12):1464-7. doi: 10.1111/j.1365-4632.2011.05009.x. 
      Epub 2014 Oct 14.