PMID- 25312844
OWN - NLM
STAT- MEDLINE
DCOM- 20150212
LR  - 20220408
IS  - 1879-1883 (Electronic)
IS  - 0002-9610 (Linking)
VI  - 208
IP  - 6
DP  - 2014 Dec
TI  - Tumor necrosis factor-alpha disruption of brain endothelial cell barrier is mediated 
      through matrix metalloproteinase-9.
PG  - 954-60; discussion 960
LID - S0002-9610(14)00450-4 [pii]
LID - 10.1016/j.amjsurg.2014.08.014 [doi]
AB  - Traumatic brain injuries cause vascular hyperpermeability. Tumor necrosis 
      factor-alpha (TNF-alpha), matrix metalloproteinase-9 (MMP-9), and caspase-3 may be 
      important in these processes but the relationship between them has not been 
      investigated. We hypothesized that TNF-alpha regulates caspase-3-mediated 
      hyperpermeability and blood brain barrier damage and hyperpermeability directly 
      or indirectly via activation of MMP-9. To test this, rat brain microvascular 
      endothelial cells were treated with TNF-alpha with or without inhibition of MMP-9. 
      Monolayer permeability was measured, zonula occludens-1 and F-actin configuration 
      were examined, and MMP-9 and caspase-3 activities were quantified. TNF-alpha 
      increased monolayer permeability, damaged zonula occludens-1, induced 
      filamentous-actin stress fiber formation, and increased both MMP-9 and caspase-3 
      activities. Inhibition of MMP-9 attenuated these changes. These data highlight a 
      novel link between TNF-alpha and MMP-9 and show that TNF-alpha regulated 
      caspase-3-mediated hyperpermeability and vascular damage may be linked to MMP-9 
      in vitro. These findings augment the understanding of traumatic brain injury and 
      pave the way for improved treatment.
CI  - Copyright (c) 2014 Elsevier Inc. All rights reserved.
FAU - Wiggins-Dohlvik, Katie
AU  - Wiggins-Dohlvik K
AD  - Department of Surgery, Baylor Scott and White Health, Temple, TX, USA; Department 
      of Surgery, Texas A&M University Health Science Center College of Medicine, 
      Temple, TX, USA.
FAU - Merriman, Morgan
AU  - Merriman M
AD  - Department of Surgery, Baylor Scott and White Health, Temple, TX, USA; Department 
      of Surgery, Texas A&M University Health Science Center College of Medicine, 
      Temple, TX, USA.
FAU - Shaji, Chinchusha A
AU  - Shaji CA
AD  - Department of Surgery, Baylor Scott and White Health, Temple, TX, USA; Department 
      of Surgery, Texas A&M University Health Science Center College of Medicine, 
      Temple, TX, USA.
FAU - Alluri, Himakarnika
AU  - Alluri H
AD  - Department of Surgery, Baylor Scott and White Health, Temple, TX, USA; Department 
      of Surgery, Texas A&M University Health Science Center College of Medicine, 
      Temple, TX, USA.
FAU - Grimsley, Marcene
AU  - Grimsley M
AD  - Department of Surgery, Baylor Scott and White Health, Temple, TX, USA; Department 
      of Surgery, Texas A&M University Health Science Center College of Medicine, 
      Temple, TX, USA.
FAU - Davis, Matthew L
AU  - Davis ML
AD  - Department of Surgery, Baylor Scott and White Health, Temple, TX, USA; Department 
      of Surgery, Texas A&M University Health Science Center College of Medicine, 
      Temple, TX, USA.
FAU - Smith, Randall W
AU  - Smith RW
AD  - Department of Surgery, Baylor Scott and White Health, Temple, TX, USA; Department 
      of Surgery, Texas A&M University Health Science Center College of Medicine, 
      Temple, TX, USA.
FAU - Tharakan, Binu
AU  - Tharakan B
AD  - Department of Surgery, Baylor Scott and White Health, Temple, TX, USA; Department 
      of Surgery, Texas A&M University Health Science Center College of Medicine, 
      Temple, TX, USA. Electronic address: btharakan@sw.org.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140928
PL  - United States
TA  - Am J Surg
JT  - American journal of surgery
JID - 0370473
RN  - 0 (Matrix Metalloproteinase Inhibitors)
RN  - 0 (TJP1 protein, human)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (Zonula Occludens-1 Protein)
RN  - EC 3.4.22.- (Caspase 3)
RN  - EC 3.4.24.35 (Matrix Metalloproteinase 9)
SB  - IM
MH  - Animals
MH  - Blood-Brain Barrier/*metabolism
MH  - Brain/*cytology
MH  - Brain Injuries/metabolism
MH  - Caspase 3/metabolism
MH  - Cells, Cultured
MH  - Endothelial Cells/metabolism
MH  - Matrix Metalloproteinase 9/*metabolism
MH  - Matrix Metalloproteinase Inhibitors/pharmacology
MH  - Rats
MH  - Tumor Necrosis Factor-alpha/*metabolism
MH  - Zonula Occludens-1 Protein/metabolism
OTO - NOTNLM
OT  - Blood brain barrier
OT  - Caspase-3
OT  - Gelatinase B
OT  - Matrix metalloproteinase
OT  - Traumatic brain injury
OT  - Tumor necrosis factor
EDAT- 2014/10/15 06:00
MHDA- 2015/02/13 06:00
CRDT- 2014/10/15 06:00
PHST- 2014/03/28 00:00 [received]
PHST- 2014/08/20 00:00 [revised]
PHST- 2014/08/25 00:00 [accepted]
PHST- 2014/10/15 06:00 [entrez]
PHST- 2014/10/15 06:00 [pubmed]
PHST- 2015/02/13 06:00 [medline]
AID - S0002-9610(14)00450-4 [pii]
AID - 10.1016/j.amjsurg.2014.08.014 [doi]
PST - ppublish
SO  - Am J Surg. 2014 Dec;208(6):954-60; discussion 960. doi: 
      10.1016/j.amjsurg.2014.08.014. Epub 2014 Sep 28.