PMID- 25316239
OWN - NLM
STAT- MEDLINE
DCOM- 20150825
LR  - 20141015
IS  - 2542-5641 (Electronic)
IS  - 0366-6999 (Linking)
VI  - 127
IP  - 20
DP  - 2014
TI  - Th and Treg response induced by Aspergillus fumigatus pulsed dendritic cells in 
      vitro.
PG  - 3616-22
AB  - BACKGROUND: Dendritic cells (DCs) can recognize the pathogen-associated molecular 
      patterns (PAMP) of Aspergillus fumigatus (A. fumigatus), activating the immune 
      response. During A. fumigatus infection, a Th and Treg response induced in the 
      fungi-pulsed DCs is not yet well understood. METHODS: In this study, bone 
      marrow-derived dendritic cells (BMDCs) were separated and proliferated from 
      C57BL/6 mice. A. fumigatus pulsed DCs were generated and cultured with CD4(+) T 
      cells derived from the spleen of C57BL/6 mice in vitro. CD4(+) T cells 
      differentiation after co-culture were analyzed by flow cytometry, ELISA, and 
      real-time PCR analysis. RESULTS: The A. fumigatus pulsed DCs exhibited increased 
      Th1 and Treg frequency, Th1-related cytokines (IFN-gamma and IL-12), Treg-related 
      cytokines (TGF-beta) and T-bet, and Foxp3 mRNA levels compared with the control 
      group. There was no significant difference between A. fumigatus pulsed DCs group 
      and the control group about Th17 and Th2 frequency. CONCLUSIONS: The inactivated 
      conidia of A. fumigatus were able to activate BMDCs and made them capable of 
      triggering T cell responses in vitro. A. fumigatus loaded DCs was a weak inducer 
      of Th17 and Th2, but induced a strong Th1 and Treg response.
FAU - Wang, Runchao
AU  - Wang R
AD  - Department of Dermatology, Research Center for Medical Mycology, Peking 
      University First Hospital, Peking University, Beijing 100034, China.
FAU - Wan, Zhe
AU  - Wan Z
AD  - Department of Dermatology, Research Center for Medical Mycology, Peking 
      University First Hospital, Peking University, Beijing 100034, China.
FAU - Li, Ruoyu
AU  - Li R
AD  - Department of Dermatology, Research Center for Medical Mycology, Peking 
      University First Hospital, Peking University, Beijing 100034, China. Email: 
      mycolab@126.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Chin Med J (Engl)
JT  - Chinese medical journal
JID - 7513795
RN  - 0 (Cytokines)
RN  - 0 (Forkhead Transcription Factors)
RN  - 0 (Foxp3 protein, mouse)
RN  - 0 (Transforming Growth Factor beta)
RN  - 187348-17-0 (Interleukin-12)
SB  - IM
MH  - Animals
MH  - Aspergillus fumigatus/*pathogenicity
MH  - Cytokines/metabolism
MH  - Dendritic Cells/*immunology/*microbiology
MH  - Forkhead Transcription Factors/metabolism
MH  - Interleukin-12/metabolism
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - T-Lymphocytes, Helper-Inducer/*immunology
MH  - T-Lymphocytes, Regulatory/*immunology
MH  - Th1 Cells/immunology
MH  - Transforming Growth Factor beta/metabolism
EDAT- 2014/10/16 06:00
MHDA- 2015/08/26 06:00
CRDT- 2014/10/16 06:00
PHST- 2014/10/16 06:00 [entrez]
PHST- 2014/10/16 06:00 [pubmed]
PHST- 2015/08/26 06:00 [medline]
PST - ppublish
SO  - Chin Med J (Engl). 2014;127(20):3616-22.