PMID- 25317018
OWN - NLM
STAT- MEDLINE
DCOM- 20150616
LR  - 20211021
IS  - 1598-6357 (Electronic)
IS  - 1011-8934 (Print)
IS  - 1011-8934 (Linking)
VI  - 29 Suppl 2
IP  - Suppl 2
DP  - 2014 Sep
TI  - Cobalt chloride attenuates oxidative stress and inflammation through NF-kappaB 
      inhibition in human renal proximal tubular epithelial cells.
PG  - S139-45
LID - 10.3346/jkms.2014.29.S2.S139 [doi]
AB  - We evaluated the effect of cobalt chloride (CoCl2) on TNF-alpha and 
      IFN-gamma-induced-inflammation and reactive oxygen species (ROS) in renal tubular 
      epithelial cells (HK-2 cells). We treated HK-2 cells with CoCl2 before the 
      administration of TNF-alpha/IFN-gamma. To regulate hemeoxygenase-1 (HO-1) expression, the 
      cells were treated CoCl2 or HO-1 siRNA. CoCl2 reduced the generation of ROS 
      induced by TNF-alpha/IFN-gamma. TNF-alpha/IFN-gamma-treated-cells showed an increase in the 
      nuclear translocation of phosphorylated NF-kappaBp65 protein, the DNA-binding 
      activity of NF-kappaBp50 and NF-kappaB transcriptional activity and a decrease in IkappaBalpha 
      protein expression. These changes were restored by CoCl2. We noted an intense 
      increase in monocyte chemoattractant protein-1 (MCP-1) and regulated on 
      activation normal T cell expressed and secreted (RANTES) production in 
      TNF-alpha/IFN-gamma-treated cells. We demonstrated that this effect was mediated through 
      NF-kappaB signaling because an NF-kappaB inhibitor significantly reduced MCP-1 and RANTES 
      production. CoCl2 effectively reduced MCP-1 and RANTES production. The expression 
      of HO-1 was increased by CoCl2 and decreased by HO-1 siRNA. However, knockdown of 
      HO-1 by RNA interference did not affect MCP-1 or RANTES production. We suggest 
      that CoCl2 has a protective effect on TNF-alpha/IFN-gamma-induced inflammation through 
      the inhibition of NF-kappaB and ROS in HK-2 cells. However, CoCl2 appears to act in 
      an HO-1-independent manner.
FAU - Oh, Se Won
AU  - Oh SW
AUID- ORCID: 0000-0003-3795-9322
AD  - Department of Internal Medicine, Inje University College of Medicine, Ilsan Paik 
      Hospital, Goyang, Korea.
FAU - Lee, Yun-Mi
AU  - Lee YM
AD  - Department of Internal Medicine, Seoul National University Bundang Hospital, 
      Seongnam, Korea.
FAU - Kim, Sejoong
AU  - Kim S
AD  - Department of Internal Medicine, Seoul National University Bundang Hospital, 
      Seongnam, Korea. ; Department of Internal Medicine, Seoul National University 
      College of Medicine, Seoul, Korea.
FAU - Chin, Ho Jun
AU  - Chin HJ
AUID- ORCID: 0000-0003-1185-2631
AD  - Department of Internal Medicine, Seoul National University Bundang Hospital, 
      Seongnam, Korea. ; Department of Internal Medicine, Seoul National University 
      College of Medicine, Seoul, Korea.
FAU - Chae, Dong-Wan
AU  - Chae DW
AD  - Department of Internal Medicine, Seoul National University Bundang Hospital, 
      Seongnam, Korea. ; Department of Internal Medicine, Seoul National University 
      College of Medicine, Seoul, Korea.
FAU - Na, Ki Young
AU  - Na KY
AUID- ORCID: 0000-0002-8872-8236
AD  - Department of Internal Medicine, Seoul National University Bundang Hospital, 
      Seongnam, Korea. ; Department of Internal Medicine, Seoul National University 
      College of Medicine, Seoul, Korea.
LA  - eng
PT  - Journal Article
DEP - 20140930
PL  - Korea (South)
TA  - J Korean Med Sci
JT  - Journal of Korean medical science
JID - 8703518
RN  - 0 (CCL2 protein, human)
RN  - 0 (CCL5 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemokine CCL5)
RN  - 0 (NF-kappa B)
RN  - 0 (NF-kappa B p50 Subunit)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (Transcription Factor RelA)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 3G0H8C9362 (Cobalt)
RN  - 82115-62-6 (Interferon-gamma)
RN  - EC 1.14.14.18 (Heme Oxygenase-1)
RN  - EVS87XF13W (cobaltous chloride)
SB  - IM
MH  - Cell Line
MH  - Chemokine CCL2/metabolism
MH  - Chemokine CCL5/metabolism
MH  - Cobalt/*pharmacology
MH  - Epithelial Cells/cytology/metabolism
MH  - Heme Oxygenase-1/antagonists & inhibitors/genetics/metabolism
MH  - Humans
MH  - *Inflammation
MH  - Interferon-gamma/pharmacology
MH  - Kidney Tubules, Proximal/cytology
MH  - NF-kappa B/antagonists & inhibitors/genetics/*metabolism
MH  - NF-kappa B p50 Subunit/genetics/metabolism
MH  - Oxidative Stress/*drug effects
MH  - Phosphorylation
MH  - Protein Binding
MH  - RNA Interference
MH  - RNA, Small Interfering/metabolism
MH  - Transcription Factor RelA/metabolism
MH  - Tumor Necrosis Factor-alpha/pharmacology
PMC - PMC4194284
OTO - NOTNLM
OT  - Cobalt Chloride
OT  - Hemeoxygenase-1
OT  - Inflammation
OT  - Nuclear Factor-kappa B
OT  - Renal Tubular Epithelial Cells
COIS- The authors have no potential conflicts of interest relevant to this article to 
      disclose.
EDAT- 2014/10/16 06:00
MHDA- 2015/06/17 06:00
PMCR- 2014/09/01
CRDT- 2014/10/16 06:00
PHST- 2014/05/10 00:00 [received]
PHST- 2014/07/03 00:00 [accepted]
PHST- 2014/10/16 06:00 [entrez]
PHST- 2014/10/16 06:00 [pubmed]
PHST- 2015/06/17 06:00 [medline]
PHST- 2014/09/01 00:00 [pmc-release]
AID - 10.3346/jkms.2014.29.S2.S139 [doi]
PST - ppublish
SO  - J Korean Med Sci. 2014 Sep;29 Suppl 2(Suppl 2):S139-45. doi: 
      10.3346/jkms.2014.29.S2.S139. Epub 2014 Sep 30.