PMID- 25319623
OWN - NLM
STAT- MEDLINE
DCOM- 20150618
LR  - 20141016
IS  - 1099-1352 (Electronic)
IS  - 0952-3499 (Linking)
VI  - 27
IP  - 12
DP  - 2014 Dec
TI  - ConBr, a lectin from Canavalia brasiliensis seeds, modulates signaling pathways 
      and increases BDNF expression probably via a glycosylated target.
PG  - 746-54
LID - 10.1002/jmr.2401 [doi]
AB  - In the central nervous system, many receptors, ion channels and neurotransmitter 
      transporters are glycoproteins, where the glycan chains are modulator elements. 
      Lectins are proteins, which recognize and bind carbohydrate complexes. We have 
      previously shown that ConBr, a lectin purified from Canavalia brasiliensis seeds, 
      produced antidepressant-like effect and blocked hippocampal neurotoxicity induced 
      by quinolinic acid and glutamate. Noteworthy, all these effects occurred in a 
      dependence of its carbohydrate recognition domain. Therefore, the present study 
      was undertaken in order to elucidate intracellular signaling pathways regulated 
      by ConBr that may be potentially associated with the antidepressant and 
      neuroprotective effects previously reported to be dependent on carbohydrate 
      interaction. ConBr (10 microg/site) was injected into the ventricle (i.c.v.) of mice, 
      and the hippocampi were removed 0.5, 1, 3, 6, 8, 12, 18, and 24 h after 
      treatment. Our results showed that in the period of 0.5-3 h, ConBr induced 
      activation of the protein kinases Akt, ERK1, and PKA. Furthermore, the 
      phosphorylation of CREB-Ser133 was stimulated by ConBr (1-6 h), while 
      brain-derived neurotrophic factor (BDNF) mRNA was increased at 12 h and BDNF 
      protein at 18-24 h. Our data suggest that an early activation of protein kinases 
      may trigger CREB-dependent BDNF transcription, resulting in a subsequent increase 
      of BDNF protein in response to ConBr. Later, increment of Akt phosphorylation was 
      observed 24 h after ConBr administration, possibly due to BDNF/TrkB-dependent 
      activation of Akt. Our findings indicate that ConBr is a multifunctional molecule 
      capable to activate signaling pathways involved in neuroplasticity and 
      neuroprotection.
CI  - Copyright (c) 2014 John Wiley & Sons, Ltd.
FAU - Rieger, Debora K
AU  - Rieger DK
AD  - Departamento de Bioquimica, Centro de Ciencias Biologicas, Universidade Federal 
      de Santa Catarina, Florianopolis, SC, 88040-900, Brazil.
FAU - Cunha, Rodrigo M S
AU  - Cunha RM
FAU - Lopes, Mark William
AU  - Lopes MW
FAU - Costa, Ana Paula
AU  - Costa AP
FAU - Budni, Josiani
AU  - Budni J
FAU - Rodrigues, Ana Lucia S
AU  - Rodrigues AL
FAU - Walz, Roger
AU  - Walz R
FAU - Teixeira, Edson H
AU  - Teixeira EH
FAU - Nascimento, Kyria S
AU  - Nascimento KS
FAU - Cavada, Benildo S
AU  - Cavada BS
FAU - Leal, Rodrigo B
AU  - Leal RB
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Mol Recognit
JT  - Journal of molecular recognition : JMR
JID - 9004580
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Cyclic AMP Response Element-Binding Protein)
RN  - 0 (Plant Lectins)
RN  - 0 (Protein Kinase Inhibitors)
RN  - 0 (RNA, Messenger)
RN  - 0 (lectin, Canavalia)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
RN  - EC 2.7.11.11 (Cyclic AMP-Dependent Protein Kinases)
RN  - EC 2.7.11.24 (Extracellular Signal-Regulated MAP Kinases)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*genetics/metabolism
MH  - Canavalia/*chemistry
MH  - Cyclic AMP Response Element-Binding Protein/metabolism
MH  - Cyclic AMP-Dependent Protein Kinases/metabolism
MH  - Extracellular Signal-Regulated MAP Kinases/metabolism
MH  - Gene Expression Regulation/drug effects
MH  - Glycosylation/drug effects
MH  - Hippocampus/drug effects/metabolism
MH  - Injections, Intraventricular
MH  - Male
MH  - Mice
MH  - Models, Biological
MH  - Phosphorylation/drug effects
MH  - Plant Lectins/*pharmacology
MH  - Protein Kinase Inhibitors/pharmacology
MH  - Proto-Oncogene Proteins c-akt/metabolism
MH  - RNA, Messenger/genetics/metabolism
MH  - Seeds/*chemistry
MH  - Signal Transduction/*drug effects
MH  - Time Factors
OTO - NOTNLM
OT  - BDNF
OT  - CREB
OT  - ConBr
OT  - lectin
OT  - neuroprotection
OT  - protein kinases
EDAT- 2014/10/17 06:00
MHDA- 2015/06/19 06:00
CRDT- 2014/10/17 06:00
PHST- 2013/10/18 00:00 [received]
PHST- 2014/05/29 00:00 [revised]
PHST- 2014/06/01 00:00 [accepted]
PHST- 2014/10/17 06:00 [entrez]
PHST- 2014/10/17 06:00 [pubmed]
PHST- 2015/06/19 06:00 [medline]
AID - 10.1002/jmr.2401 [doi]
PST - ppublish
SO  - J Mol Recognit. 2014 Dec;27(12):746-54. doi: 10.1002/jmr.2401.