PMID- 25320036
OWN - NLM
STAT- MEDLINE
DCOM- 20150129
LR  - 20141016
IS  - 0177-5103 (Print)
IS  - 0177-5103 (Linking)
VI  - 111
IP  - 3
DP  - 2014 Oct 16
TI  - Biochemical reference intervals and pathophysiological changes in Flavobacterium 
      psychrophilum-resistant and -susceptible rainbow trout lines.
PG  - 239-48
LID - 10.3354/dao02777 [doi]
AB  - Host genetic resistance against disease-causing pathogens can be enhanced through 
      family-based selective breeding. At present, there is an incomplete understanding 
      of how artificial selection of fish alters host physiology and response following 
      pathogen exposure. We previously reported the generation of selectively-bred 
      rainbow trout Oncorhynchus mykiss lines with either increased resistance 
      (ARS-Fp-R) or susceptibility (ARS-Fp-S) to bacterial cold water disease (BCWD). 
      This study (1) determined baseline reference-range intervals for packed cell 
      volume (PCV) and 18 plasma biochemistry analytes, and (2) examined 
      pathophysiological changes following infection between the genetic lines. PCV and 
      biochemistry reference-range intervals did not significantly differ between 
      genetic lines; thus data were pooled into a single reference-range population (n 
      = 85). ARS-Fp-R and ARS-Fp-S line fish were intraperitoneally challenged with 
      Flavobacterium psychrophilum, and plasma was collected on Days 1, 3, 6, and 9 
      post-challenge. Splenic bacterial load was measured using an F. 
      psychrophilum-specific qPCR assay. In both genetic lines, changes were observed 
      in mean PCV, total protein, albumin, glucose, cholesterol, chloride, and calcium, 
      falling outside the established reference intervals and significantly differing 
      from phosphate-buffered saline challenged fish, on at least 1d post-challenge. 
      Mean PCV, total protein, and calcium significantly differed between ARS-Fp-R and 
      ARS-Fp-S line fish on Day 9 post-infection, with values in the ARS-Fp-S line 
      deviating most from the reference interval. PCV, total protein, cholesterol, and 
      calcium negatively correlated with bacterial load. These findings identify 
      divergent pathophysiological responses between ARS-Fp-R and ARS-Fp-S line fish 
      following laboratory challenge that are likely associated with differential 
      survival.
FAU - Marancik, David P
AU  - Marancik DP
AD  - National Center for Cool and Cold Water Aquaculture, USDA-ARS, 11861 Leetown Rd, 
      Kearneysville, WV 25430, USA.
FAU - Camus, Melinda S
AU  - Camus MS
FAU - Camus, Alvin C
AU  - Camus AC
FAU - Leeds, Timothy D
AU  - Leeds TD
FAU - Weber, Gregory M
AU  - Weber GM
FAU - Wiens, Gregory D
AU  - Wiens GD
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - Germany
TA  - Dis Aquat Organ
JT  - Diseases of aquatic organisms
JID - 8807037
SB  - IM
MH  - Animals
MH  - Breeding
MH  - Fish Diseases/genetics/metabolism/*microbiology/pathology
MH  - Flavobacteriaceae Infections/microbiology/pathology/*veterinary
MH  - Flavobacterium/*classification
MH  - *Genetic Predisposition to Disease
MH  - Oncorhynchus mykiss/*genetics
EDAT- 2014/10/17 06:00
MHDA- 2015/01/30 06:00
CRDT- 2014/10/17 06:00
PHST- 2014/10/17 06:00 [entrez]
PHST- 2014/10/17 06:00 [pubmed]
PHST- 2015/01/30 06:00 [medline]
AID - 10.3354/dao02777 [doi]
PST - ppublish
SO  - Dis Aquat Organ. 2014 Oct 16;111(3):239-48. doi: 10.3354/dao02777.