PMID- 25320376
OWN - NLM
STAT- MEDLINE
DCOM- 20150219
LR  - 20190722
IS  - 1530-8561 (Electronic)
IS  - 0009-9147 (Linking)
VI  - 60
IP  - 12
DP  - 2014 Dec
TI  - Detection of clonal evolution in hematopoietic malignancies by combining 
      comparative genomic hybridization and single nucleotide polymorphism arrays.
PG  - 1558-68
LID - 10.1373/clinchem.2014.227785 [doi]
AB  - BACKGROUND: Array comparative genomic hybridization (aCGH) has become a powerful 
      tool for analyzing hematopoietic neoplasms and identifying genome-wide copy 
      number changes in a single assay. aCGH also has superior resolution compared with 
      fluorescence in situ hybridization (FISH) or conventional cytogenetics. 
      Integration of single nucleotide polymorphism (SNP) probes with microarray 
      analysis allows additional identification of acquired uniparental disomy, a copy 
      neutral aberration with known potential to contribute to tumor pathogenesis. 
      However, a limitation of microarray analysis has been the inability to detect 
      clonal heterogeneity in a sample. METHODS: This study comprised 16 samples (acute 
      myeloid leukemia, myelodysplastic syndrome, chronic lymphocytic leukemia, plasma 
      cell neoplasm) with complex cytogenetic features and evidence of clonal 
      evolution. We used an integrated manual peak reassignment approach combining 
      analysis of aCGH and SNP microarray data for characterization of subclonal 
      abnormalities. We compared array findings with results obtained from conventional 
      cytogenetic and FISH studies. RESULTS: Clonal heterogeneity was detected in 13 of 
      16 samples by microarray on the basis of log2 values. Use of the manual peak 
      reassignment analysis approach improved resolution of the sample's clonal 
      composition and genetic heterogeneity in 10 of 13 (77%) patients. Moreover, in 3 
      patients, clonal disease progression was revealed by array analysis that was not 
      evident by cytogenetic or FISH studies. CONCLUSIONS: Genetic abnormalities 
      originating from separate clonal subpopulations can be identified and further 
      characterized by combining aCGH and SNP hybridization results from 1 integrated 
      microarray chip by use of the manual peak reassignment technique. Its clinical 
      utility in comparison to conventional cytogenetic or FISH studies is 
      demonstrated.
CI  - (c) 2014 American Association for Clinical Chemistry.
FAU - Hartmann, Luise
AU  - Hartmann L
AD  - HematoLogics Inc., Seattle, WA.
FAU - Stephenson, Christine F
AU  - Stephenson CF
AD  - HematoLogics Inc., Seattle, WA.
FAU - Verkamp, Stephanie R
AU  - Verkamp SR
AD  - HematoLogics Inc., Seattle, WA.
FAU - Johnson, Krystal R
AU  - Johnson KR
AD  - HematoLogics Inc., Seattle, WA.
FAU - Burnworth, Bettina
AU  - Burnworth B
AD  - HematoLogics Inc., Seattle, WA.
FAU - Hammock, Kelle
AU  - Hammock K
AD  - HematoLogics Inc., Seattle, WA.
FAU - Brodersen, Lisa Eidenschink
AU  - Brodersen LE
AD  - HematoLogics Inc., Seattle, WA.
FAU - de Baca, Monica E
AU  - de Baca ME
AD  - HematoLogics Inc., Seattle, WA.
FAU - Wells, Denise A
AU  - Wells DA
AD  - HematoLogics Inc., Seattle, WA.
FAU - Loken, Michael R
AU  - Loken MR
AD  - HematoLogics Inc., Seattle, WA.
FAU - Zehentner, Barbara K
AU  - Zehentner BK
AD  - HematoLogics Inc., Seattle, WA. barbara@hematologics.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20141015
PL  - England
TA  - Clin Chem
JT  - Clinical chemistry
JID - 9421549
SB  - IM
MH  - Clonal Evolution/*genetics
MH  - *Comparative Genomic Hybridization
MH  - Hematologic Neoplasms/*genetics/*pathology
MH  - Humans
MH  - *Oligonucleotide Array Sequence Analysis
MH  - Polymorphism, Single Nucleotide/*genetics
EDAT- 2014/10/17 06:00
MHDA- 2015/02/20 06:00
CRDT- 2014/10/17 06:00
PHST- 2014/10/17 06:00 [entrez]
PHST- 2014/10/17 06:00 [pubmed]
PHST- 2015/02/20 06:00 [medline]
AID - clinchem.2014.227785 [pii]
AID - 10.1373/clinchem.2014.227785 [doi]
PST - ppublish
SO  - Clin Chem. 2014 Dec;60(12):1558-68. doi: 10.1373/clinchem.2014.227785. Epub 2014 
      Oct 15.