PMID- 25320454
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20141016
LR  - 20211021
IS  - 0912-0009 (Print)
IS  - 1880-5086 (Electronic)
IS  - 0912-0009 (Linking)
VI  - 55
IP  - 2
DP  - 2014 Sep
TI  - Prosaposin regulates coenzyme Q10 levels in HepG2 cells, especially those in 
      mitochondria.
PG  - 85-9
LID - 10.3164/jcbn.13-106 [doi]
AB  - Coenzyme Q10 (CoQ10) is a key component of the mitochondrial electron transfer 
      chain and is one of the most important cellular antioxidants. We previously 
      reported that glycoprotein saposin B (SapB) binds CoQ10 in human cells. To 
      elucidate the physiological role of SapB and its precursor, prosaposin (Psap), we 
      prepared stable transfectants of HepG2 that overexpress wild-type human Psap 
      (Wt-Tf). We also established a SapB domain mutated Psap (Mt-Tf) in which 
      cysteine(198) was replaced with serine to disrupt three dimensional protein 
      structure by the loss of S-S bridging. Psap knockdown (KD) strains were also 
      examined. Western blotting analysis confirmed overexpression or knockdown of Psap 
      in these HepG2 cells. The cellular ratios of CoQ10 to free cholesterol (FC) 
      significantly decreased in the order of Wt-Tf>parental>Mt-Tf>KD. Additionally, 
      the ratios of CoQ10/FC in mitochondrial fractions decreased in the order of 
      Wt-Tf>parental>KD. These data indicate that Psap and/or SapB regulate CoQ10 
      levels in HepG2 cells, especially in their mitochondria.
FAU - Kashiba, Misato
AU  - Kashiba M
AD  - School of Health Sciences, Tokyo University of Technology, 1404-1 Katakura-cho, 
      Hachioji, Tokyo 192-0982, Japan.
FAU - Oizumi, Mikiko
AU  - Oizumi M
AD  - School of Bioscience and Biotechnology, Tokyo University of Technology, 1404-1 
      Katakura-cho, Hachioji, Tokyo 192-0982, Japan.
FAU - Suzuki, Masaru
AU  - Suzuki M
AD  - School of Bioscience and Biotechnology, Tokyo University of Technology, 1404-1 
      Katakura-cho, Hachioji, Tokyo 192-0982, Japan.
FAU - Sawamura, Yoshimi
AU  - Sawamura Y
AD  - School of Bioscience and Biotechnology, Tokyo University of Technology, 1404-1 
      Katakura-cho, Hachioji, Tokyo 192-0982, Japan.
FAU - Nagashima, Kohei
AU  - Nagashima K
AD  - School of Bioscience and Biotechnology, Tokyo University of Technology, 1404-1 
      Katakura-cho, Hachioji, Tokyo 192-0982, Japan.
FAU - Yoshimura, Shinichi
AU  - Yoshimura S
AD  - School of Bioscience and Biotechnology, Tokyo University of Technology, 1404-1 
      Katakura-cho, Hachioji, Tokyo 192-0982, Japan.
FAU - Yamamoto, Yorihiro
AU  - Yamamoto Y
AD  - School of Bioscience and Biotechnology, Tokyo University of Technology, 1404-1 
      Katakura-cho, Hachioji, Tokyo 192-0982, Japan.
LA  - eng
PT  - Journal Article
DEP - 20140704
PL  - Japan
TA  - J Clin Biochem Nutr
JT  - Journal of clinical biochemistry and nutrition
JID - 8700907
PMC - PMC4186375
OTO - NOTNLM
OT  - HepG2
OT  - coenzyme Q10
OT  - mitochondria
OT  - prosaposin
OT  - saposin B
EDAT- 2014/10/17 06:00
MHDA- 2014/10/17 06:01
PMCR- 2014/09/01
CRDT- 2014/10/17 06:00
PHST- 2013/12/03 00:00 [received]
PHST- 2014/04/24 00:00 [accepted]
PHST- 2014/10/17 06:00 [entrez]
PHST- 2014/10/17 06:00 [pubmed]
PHST- 2014/10/17 06:01 [medline]
PHST- 2014/09/01 00:00 [pmc-release]
AID - jcbn13-106 [pii]
AID - 10.3164/jcbn.13-106 [doi]
PST - ppublish
SO  - J Clin Biochem Nutr. 2014 Sep;55(2):85-9. doi: 10.3164/jcbn.13-106. Epub 2014 Jul 
      4.