PMID- 25323759
OWN - NLM
STAT- MEDLINE
DCOM- 20150819
LR  - 20190221
IS  - 1898-4002 (Electronic)
IS  - 1896-1126 (Linking)
VI  - 59
IP  - 2
DP  - 2014 Sep
TI  - Immunohistochemical expression of p27(kip1) in metastatic laryngeal squamous cell 
      carcinoma.
PG  - 206-12
LID - S1896-1126(14)00050-9 [pii]
LID - 10.1016/j.advms.2014.03.005 [doi]
AB  - PURPOSE: Laryngeal squamous cell carcinoma (LSCC) is an interesting diagnostic 
      and therapeutic issue. The diagnostic delay is mainly a consequence of the lack 
      of evident symptoms in the early stage of the disease. The purpose of current 
      studies was the evaluation of the expression of p27(kip1) in primary and 
      metastatic LSCC in correlation with patients' clinicopathological data. 
      MATERIAL/METHODS: The indirect immunohistochemical studies were performed on the 
      series of 60 sections (primary tumor: 20 cases of N(0) and 20 cases of N(+), and 
      nodal meta: 20 cases), using primary antibody against p27(kip1) [clone 1B4]. The 
      expression of analyzed protein was performed using automated morphometric 
      methods. RESULTS: The p27(kip1) nuclear expression was found in 100% (40/40) 
      cases of primary tumor, and in 85% (17/20) cases of SCC meta at lymph nodes. In 
      primary LSCC N(0) the expression of p27(kip1) was significantly higher compared 
      to N(+) cases (p=0.036672). However, the p27(kip1) expression in SCC metastases 
      was higher compared to the primary SCC. Moreover, the analyses based on the 
      classification trees revealed the cutoff p27(kip1) expression in primary LSCC 
      (IRS </= 76) which was characteristic for N(+) patients. Consequently, our analysis 
      revealed that high expression of p27(kip1) (IRS>76) was characteristic for N(0) 
      patients. CONCLUSIONS: Our results suggest that p27(kip1) might be useful 
      prognostic factor of metastatic potential in laryngeal squamous cell carcinoma.
CI  - Copyright (c) 2014 Medical University of Bialystok. Published by Elsevier Urban & 
      Partner Sp. z o.o. All rights reserved.
FAU - Bodnar, Magdalena
AU  - Bodnar M
AD  - Department of Clinical Pathomorphology, Collegium Medicum in Bydgoszcz, Nicolaus 
      Copernicus University in Torun, Bydgoszcz, Poland.
FAU - Szylberg, Lukasz
AU  - Szylberg L
AD  - Department of Clinical Pathomorphology, Collegium Medicum in Bydgoszcz, Nicolaus 
      Copernicus University in Torun, Bydgoszcz, Poland.
FAU - Kazmierczak, Wojciech
AU  - Kazmierczak W
AD  - Department of Otolaryngology and Clinical Oncology Chair and Clinic of 
      Otolaryngology and Department of Pathophysiology of Hearing and Balance System 
      Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Torun, 
      Bydgoszcz, Poland.
FAU - Marszalek, Andrzej
AU  - Marszalek A
AD  - Department of Clinical Pathomorphology, Collegium Medicum in Bydgoszcz, Nicolaus 
      Copernicus University in Torun, Bydgoszcz, Poland; Department of Oncologic 
      Pathology, Poznan University of Medical Sciences and Greater Poland Oncology 
      Center, Poznan, Poland. Electronic address: amars@cm.umk.pl.
LA  - eng
PT  - Comparative Study
PT  - Journal Article
DEP - 20140609
PL  - Netherlands
TA  - Adv Med Sci
JT  - Advances in medical sciences
JID - 101276222
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (CDKN1B protein, human)
RN  - 147604-94-2 (Cyclin-Dependent Kinase Inhibitor p27)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Biomarkers, Tumor/*metabolism
MH  - Carcinoma, Squamous Cell/*metabolism/pathology/secondary
MH  - Cyclin-Dependent Kinase Inhibitor p27/*metabolism
MH  - *Down-Regulation
MH  - Female
MH  - Head and Neck Neoplasms/metabolism/pathology
MH  - Humans
MH  - Laryngeal Neoplasms/*metabolism/pathology
MH  - Larynx/*metabolism/pathology
MH  - Lymph Nodes/*metabolism/pathology
MH  - Lymphatic Metastasis
MH  - Male
MH  - Middle Aged
MH  - Neoplasm Staging
MH  - Squamous Cell Carcinoma of Head and Neck
OTO - NOTNLM
OT  - Immunohistochemistry
OT  - Laryngeal squamous cell carcinoma
OT  - Metastases
OT  - Morphometric methods
OT  - p27(kip1)
EDAT- 2014/10/18 06:00
MHDA- 2015/08/20 06:00
CRDT- 2014/10/18 06:00
PHST- 2013/10/18 00:00 [received]
PHST- 2014/03/14 00:00 [accepted]
PHST- 2014/10/18 06:00 [entrez]
PHST- 2014/10/18 06:00 [pubmed]
PHST- 2015/08/20 06:00 [medline]
AID - S1896-1126(14)00050-9 [pii]
AID - 10.1016/j.advms.2014.03.005 [doi]
PST - ppublish
SO  - Adv Med Sci. 2014 Sep;59(2):206-12. doi: 10.1016/j.advms.2014.03.005. Epub 2014 
      Jun 9.