PMID- 25324284
OWN - NLM
STAT- MEDLINE
DCOM- 20150126
LR  - 20141204
IS  - 1552-5783 (Electronic)
IS  - 0146-0404 (Linking)
VI  - 55
IP  - 12
DP  - 2014 Oct 16
TI  - Biological features of herpes simplex virus type 1 latency in mice according to 
      experimental conditions and type of neurones.
PG  - 7761-74
LID - 10.1167/iovs.14-14673 [doi]
AB  - PURPOSE: To compare the biological patterns of viral transcripts during herpes 
      simplex virus type 1 (HSV1) latent infection according to experimental 
      conditions. METHODS: Two types of murine models of HSV1 infection were used: the 
      corneal scarification model, often used in studies of HSV1 latency, and the 
      oro-ocular murine model. Two strains of HSV1 were used for the inoculation: SC16, 
      a wild type strain considered as highly neuroinvasive, and KOS, previously 
      described as poorly neurovirulent. The amounts of viral genomes, and those of 
      four types of viral transcripts (immediate-early, early and late, together with 
      latency-associated transcripts [LATs]), were measured by quantitative PCR and 
      RT-PCR in the main sites of HSV1 latent infection at 6 days, 1 and 3 months post 
      inoculation, and the number of LAT-expressing neurons was assessed by in-situ 
      hybridization on histological sections of trigeminal ganglia (TG). RESULTS: Using 
      the SC16 strain of HSV1 in the oro-ocular model, immediate-early transcripts were 
      still present at 1 month post inoculation (early stage of latent infection), but 
      were not detected at 3 months (late stage of latent infection). In both cases, 
      early and late viral genes transcripts were not detected, demonstrating the 
      latent nature of the infection with this combination of experimental conditions. 
      In contrast, such progress in the viral gene expression was not observed in the 
      corneal scarification model, particularly when the KOS strain of HSV1 was used. 
      CONCLUSIONS: These results highlight that the behavior of the virus in the 
      nervous system depends on the method inoculation, and the viral strain. All these 
      parameters are likely to influence the establishment of latent infection.
CI  - Copyright 2014 The Association for Research in Vision and Ophthalmology, Inc.
FAU - Cavallero, Sophie
AU  - Cavallero S
AD  - Laboratoire de Virologie Moleculaire et Structurale, Centre National de la 
      Recherche Scientifique, Gif sur Yvette, France.
FAU - Huot, Nicolas
AU  - Huot N
AD  - Laboratoire de Virologie Moleculaire et Structurale, Centre National de la 
      Recherche Scientifique, Gif sur Yvette, France.
FAU - Francelle, Laetitia
AU  - Francelle L
AD  - Laboratoire de Virologie Moleculaire et Structurale, Centre National de la 
      Recherche Scientifique, Gif sur Yvette, France.
FAU - Lomonte, Patrick
AU  - Lomonte P
AD  - Centre de Genetique et Physiologie Moleculaire et Cellulaire CNRS, UMR5534, 
      Universite de Lyon 1, Lyon, France, Laboratoire d'excellence, Labex DEVweCAN, 
      Lyon, France.
FAU - Naas, Thierry
AU  - Naas T
AD  - Service de Bacteriologie-Virologie-Hygiene, INSERM U914 "Emerging Resistance to 
      Antibiotics," Hopital de Bicetre, Assistance Publique / Hopitaux de Paris, 
      Faculte de Medecine and Universite Paris Sud, Kremlin-Bicetre, France.
FAU - Labetoulle, Marc
AU  - Labetoulle M
AD  - Laboratoire de Virologie Moleculaire et Structurale, Centre National de la 
      Recherche Scientifique, Gif sur Yvette, France Service d'Ophtalmologie, Hopital 
      de Bicetre, Assistance Publique-Hopitaux de Paris, Assistance Publique / Hopitaux 
      de Paris, Faculte de Medecine and Universite Paris Sud, Kremlin-Bicetre, France.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20141016
PL  - United States
TA  - Invest Ophthalmol Vis Sci
JT  - Investigative ophthalmology & visual science
JID - 7703701
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (Lat protein, mouse)
RN  - 0 (Membrane Proteins)
RN  - 0 (Phosphoproteins)
SB  - IM
MH  - Adaptor Proteins, Signal Transducing/metabolism
MH  - Animals
MH  - Corneal Diseases/*virology
MH  - Disease Models, Animal
MH  - Eye Infections, Viral/mortality/*virology
MH  - Female
MH  - Flow Cytometry
MH  - Gene Expression Regulation, Viral
MH  - Herpes Simplex/mortality/*virology
MH  - Herpesvirus 1, Human/*physiology
MH  - Membrane Proteins/metabolism
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Neurons/metabolism/*virology
MH  - Phosphoproteins/metabolism
MH  - Polymerase Chain Reaction/methods
MH  - *Virus Latency
MH  - Virus Replication/physiology
OTO - NOTNLM
OT  - HSV1
OT  - ICP4
OT  - LAT
OT  - latency
OT  - virulence
EDAT- 2014/10/18 06:00
MHDA- 2015/01/27 06:00
CRDT- 2014/10/18 06:00
PHST- 2014/10/18 06:00 [entrez]
PHST- 2014/10/18 06:00 [pubmed]
PHST- 2015/01/27 06:00 [medline]
AID - iovs.14-14673 [pii]
AID - 10.1167/iovs.14-14673 [doi]
PST - epublish
SO  - Invest Ophthalmol Vis Sci. 2014 Oct 16;55(12):7761-74. doi: 
      10.1167/iovs.14-14673.