PMID- 25326299
OWN - NLM
STAT- MEDLINE
DCOM- 20150803
LR  - 20220408
IS  - 1098-5336 (Electronic)
IS  - 0099-2240 (Print)
IS  - 0099-2240 (Linking)
VI  - 81
IP  - 1
DP  - 2015 Jan
TI  - Enhancing Terpene yield from sugars via novel routes to 1-deoxy-d-xylulose 
      5-phosphate.
PG  - 130-8
LID - 10.1128/AEM.02920-14 [doi]
AB  - Terpene synthesis in the majority of bacterial species, together with plant 
      plastids, takes place via the 1-deoxy-d-xylulose 5-phosphate (DXP) pathway. The 
      first step of this pathway involves the condensation of pyruvate and 
      glyceraldehyde 3-phosphate by DXP synthase (Dxs), with one-sixth of the carbon 
      lost as CO2. A hypothetical novel route from a pentose phosphate to DXP (nDXP) 
      could enable a more direct pathway from C5 sugars to terpenes and also circumvent 
      regulatory mechanisms that control Dxs, but there is no enzyme known that can 
      convert a sugar into its 1-deoxy equivalent. Employing a selection for 
      complementation of a dxs deletion in Escherichia coli grown on xylose as the sole 
      carbon source, we uncovered two candidate nDXP genes. Complementation was 
      achieved either via overexpression of the wild-type E. coli yajO gene, annotated 
      as a putative xylose reductase, or via various mutations in the native ribB gene. 
      In vitro analysis performed with purified YajO and mutant RibB proteins revealed 
      that DXP was synthesized in both cases from ribulose 5-phosphate (Ru5P). We 
      demonstrate the utility of these genes for microbial terpene biosynthesis by 
      engineering the DXP pathway in E. coli for production of the sesquiterpene 
      bisabolene, a candidate biodiesel. To further improve flux into the pathway from 
      Ru5P, nDXP enzymes were expressed as fusions to DXP reductase (Dxr), the second 
      enzyme in the DXP pathway. Expression of a Dxr-RibB(G108S) fusion improved 
      bisabolene titers more than 4-fold and alleviated accumulation of intracellular 
      DXP.
CI  - Copyright (c) 2015, American Society for Microbiology. All Rights Reserved.
FAU - Kirby, James
AU  - Kirby J
AD  - California Institute of Quantitative Biosciences (QB3), University of California, 
      Berkeley, Berkeley, California, USA Joint BioEnergy Institute, Emeryville, 
      California, USA.
FAU - Nishimoto, Minobu
AU  - Nishimoto M
AD  - California Institute of Quantitative Biosciences (QB3), University of California, 
      Berkeley, Berkeley, California, USA Joint BioEnergy Institute, Emeryville, 
      California, USA.
FAU - Chow, Ruthie W N
AU  - Chow RW
AD  - California Institute of Quantitative Biosciences (QB3), University of California, 
      Berkeley, Berkeley, California, USA.
FAU - Baidoo, Edward E K
AU  - Baidoo EE
AD  - Joint BioEnergy Institute, Emeryville, California, USA.
FAU - Wang, George
AU  - Wang G
AD  - Joint BioEnergy Institute, Emeryville, California, USA.
FAU - Martin, Joel
AU  - Martin J
AD  - Joint Genome Institute, Walnut Creek, California, USA.
FAU - Schackwitz, Wendy
AU  - Schackwitz W
AD  - Joint Genome Institute, Walnut Creek, California, USA.
FAU - Chan, Rossana
AU  - Chan R
AD  - California Institute of Quantitative Biosciences (QB3), University of California, 
      Berkeley, Berkeley, California, USA.
FAU - Fortman, Jeffrey L
AU  - Fortman JL
AD  - California Institute of Quantitative Biosciences (QB3), University of California, 
      Berkeley, Berkeley, California, USA.
FAU - Keasling, Jay D
AU  - Keasling JD
AD  - California Institute of Quantitative Biosciences (QB3), University of California, 
      Berkeley, Berkeley, California, USA Joint BioEnergy Institute, Emeryville, 
      California, USA Department of Chemical and Biomolecular Engineering, University 
      of California, Berkeley, Berkeley, California, USA Physical Biosciences Division, 
      Lawrence Berkeley National Laboratory, Berkeley, California, USA 
      keasling@berkeley.edu.
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20141017
PL  - United States
TA  - Appl Environ Microbiol
JT  - Applied and environmental microbiology
JID - 7605801
RN  - 0 (1-deoxylulose 5-phosphate)
RN  - 0 (Pentosephosphates)
RN  - 0 (Terpenes)
RN  - A1TA934AKO (Xylose)
SB  - IM
MH  - Biotransformation
MH  - Escherichia coli/*genetics/*metabolism
MH  - Genetic Complementation Test
MH  - *Metabolic Engineering
MH  - Pentosephosphates/*metabolism
MH  - Terpenes/*metabolism
MH  - Xylose/*metabolism
PMC - PMC4272731
EDAT- 2014/10/19 06:00
MHDA- 2015/08/04 06:00
PMCR- 2015/07/01
CRDT- 2014/10/19 06:00
PHST- 2014/10/19 06:00 [entrez]
PHST- 2014/10/19 06:00 [pubmed]
PHST- 2015/08/04 06:00 [medline]
PHST- 2015/07/01 00:00 [pmc-release]
AID - AEM.02920-14 [pii]
AID - 02920-14 [pii]
AID - 10.1128/AEM.02920-14 [doi]
PST - ppublish
SO  - Appl Environ Microbiol. 2015 Jan;81(1):130-8. doi: 10.1128/AEM.02920-14. Epub 
      2014 Oct 17.