PMID- 25327654
OWN - NLM
STAT- MEDLINE
DCOM- 20150411
LR  - 20190221
IS  - 0253-3766 (Print)
IS  - 0253-3766 (Linking)
VI  - 36
IP  - 7
DP  - 2014 Jul
TI  - [ALK protein expression and gene fusion in bronchoscopic specimens of lung 
      adenocarcinoma].
PG  - 501-4
AB  - OBJECTIVE: To explore ALK protein expression and gene fusion in formalin-fixed 
      and paraffin-embedded (FFPE) specimens obtained from lung cancer by bronchoscopy, 
      and to investigate the relationship between ALK status and clinicopathological 
      characteristics of the patients. METHODS: Seventy-four FFPE samples obtained from 
      lung adenocarcinoma by bronchoscopy were tested for ALK protein expression and 
      gene fusion respectively by immunohistochemistry (IHC) using Ventana D5F3 
      antibody and fluorescence in situ hybridization (FISH) using ALK break apart 
      probe. RESULTS: sixty-five of the 74 samples were successfully tested by FISH 
      (87.8%, 65/74) . There were 5 FISH-positive cases (7.7%, 5/65) , all with 
      advanced stage carcinoma. Among these five FISH-positive cases, 3 were 
      IHC-positive (4.1%, 3/74) and 2 IHC-negative cases. All the other 69 samples were 
      IHC-negative, including nine FISH-uninformative samples (7 samples were less than 
      50 tumor cells and 2 samples with weak FISH signal). Both ALK IHC and FISH 
      results were not correlated with age, sex, history of smoking, histological 
      classification, differentiation and lymph node metastasis. CONCLUSIONS: 
      Bronchoscopic specimens of lung cancer can be used to detect ALK expression and 
      gene fusion. Immunohistochemistry in combination with FISH test may be more 
      favorable for ALK test.
FAU - Liang, Xiaolong
AU  - Liang X
AD  - Department of Pathology of Peking Union Hospital, Chinese Academy of Medical 
      Sciences and Peking Union Medical College, Beijing 100730, China.
FAU - Wang, Mengzhao
AU  - Wang M
FAU - Zhang, Jing
AU  - Zhang J
FAU - Luo, Yufeng
AU  - Luo Y
FAU - Zhang, Shuying
AU  - Zhang S
FAU - Wu, Shafei
AU  - Wu S
FAU - Liu, Yuanyuan
AU  - Liu Y
FAU - Zeng, Xuan
AU  - Zeng X
AD  - Email: zengxuan88@yahoo.com.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhonghua Zhong Liu Za Zhi
JT  - Zhonghua zhong liu za zhi [Chinese journal of oncology]
JID - 7910681
RN  - EC 2.7.10.1 (ALK protein, human)
RN  - EC 2.7.10.1 (Anaplastic Lymphoma Kinase)
RN  - EC 2.7.10.1 (Receptor Protein-Tyrosine Kinases)
SB  - IM
MH  - Adenocarcinoma/*metabolism
MH  - Adenocarcinoma of Lung
MH  - Anaplastic Lymphoma Kinase
MH  - Gene Fusion
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization, Fluorescence
MH  - Lung Neoplasms/*metabolism
MH  - Receptor Protein-Tyrosine Kinases/*metabolism
EDAT- 2014/10/21 06:00
MHDA- 2015/04/12 06:00
CRDT- 2014/10/21 06:00
PHST- 2014/10/21 06:00 [entrez]
PHST- 2014/10/21 06:00 [pubmed]
PHST- 2015/04/12 06:00 [medline]
PST - ppublish
SO  - Zhonghua Zhong Liu Za Zhi. 2014 Jul;36(7):501-4.