PMID- 25327797
OWN - NLM
STAT- MEDLINE
DCOM- 20150505
LR  - 20160818
IS  - 0529-5807 (Print)
IS  - 0529-5807 (Linking)
VI  - 43
IP  - 7
DP  - 2014 Jul
TI  - [Delay in formalin fixation and HER2 testing in gastric cancer].
PG  - 468-72
AB  - OBJECTIVE: To evaluated HER2 status using immunohistochemistry (IHC) assay and 
      fluorescence in situ hybridization (FISH) at two different time points of tissue 
      fixation after surgical resection of gastric cancer, emphasizing the importance 
      of standard operation and quality control in HER2 testing. METHODS: Forty-one 
      resection specimens of advanced gastric cancer were collected with tissue 
      fixation periods of < 30 min or > 30 min after surgical resection. HER2 status 
      was evaluated by immunohistochemistry (IHC) assay and fluorescence in situ 
      hybridization (FISH). RESULTS: The frequency of HER2 expression by IHC in the 
      samples with fixation time of < 30 min was higher than that in those of > 30 min 
      (P < 0.05). However, no significant difference was observed by FISH (P > 0.05) 
      between the two groups. Samples of < 30 min fixation time had high concordant 
      results between IHC and FISH (100.0% for both positive and negative cases, Rho = 
      0.724, P < 0.05). In addition, HER2 expression by IHC was significantly 
      correlated with Lauren classification, histologic differentiation, TNM stage and 
      gender (P < 0.05). CONCLUSION: The time to tissue fixation after surgical 
      resection of more than 30 min has deleterious effect on the detection of HER2 by 
      IHC although FISH testing is not affected.
FAU - Zeng, Lixia
AU  - Zeng L
AD  - Department of Pathology, Affiliated Tumor Hospital of Guangxi Medical University, 
      Nanning 530021, China.
FAU - Huang, Junqi
AU  - Huang J
FAU - Ma, Yun
AU  - Ma Y
FAU - Liu, Yixiao
AU  - Liu Y
FAU - Wei, Yuying
AU  - Wei Y
FAU - Zheng, Qian
AU  - Zheng Q
FAU - Ye, Hongtao
AU  - Ye H
AD  - E-mail: dr.hongtaoye@googlemail.com.
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhonghua Bing Li Xue Za Zhi
JT  - Zhonghua bing li xue za zhi = Chinese journal of pathology
JID - 0005331
RN  - EC 2.7.10.1 (Receptor, ErbB-2)
SB  - IM
MH  - Aged
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization, Fluorescence
MH  - Receptor, ErbB-2/*analysis
MH  - Stomach Neoplasms/*chemistry/pathology/surgery
MH  - Time Factors
MH  - Tissue Fixation/*methods
EDAT- 2014/10/21 06:00
MHDA- 2015/05/06 06:00
CRDT- 2014/10/21 06:00
PHST- 2014/10/21 06:00 [entrez]
PHST- 2014/10/21 06:00 [pubmed]
PHST- 2015/05/06 06:00 [medline]
PST - ppublish
SO  - Zhonghua Bing Li Xue Za Zhi. 2014 Jul;43(7):468-72.