PMID- 25330519
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20151026
LR  - 20211021
IS  - 1300-7777 (Print)
IS  - 1308-5263 (Electronic)
IS  - 1300-7777 (Linking)
VI  - 31
IP  - 3
DP  - 2014 Sep 5
TI  - Critical role of sensitized serum in rejection of allogeneic bone marrow cells.
PG  - 266-71
LID - 10.4274/tjh.2012.0213 [doi]
AB  - OBJECTIVE: Humoral immunity has been clearly implicated in solid organ 
      transplantation, but little is known about the relationship between humoral 
      immunity and hematopoietic stem cell transplantation. This study was designed to 
      investigate that relationship. MATERIALS AND METHODS: Sensitized serum was 
      obtained from a sensitized murine model established by allogeneic splenocyte 
      transfusion. Sensitized serum was incubated with allogeneic bone marrow cells 
      (BMCs) in vitro and the cytotoxicity was evaluated by the complement-dependent 
      cytotoxicity method. Mice were transplanted with allogeneic BMCs incubated with 
      sensitized serum after lethal irradiation. The engraftment was assayed by 
      hematopoietic recovery and chimera analysis. Moreover, mice received passive 
      transfer of sensitized serum 1 day prior to transplantation. Mortality was scored 
      daily after bone marrow transplantation. RESULTS: The in vitro experiments showed 
      that sensitized serum was capable of impairing allogeneic BMCs through the 
      complement-dependent cytotoxicity pathway. The animal studies showed that BMCs 
      incubated with sensitized serum failed to rescue mice from lethal irradiation. 
      The engraftment assay showed that the allogeneic BMCs incubated with sensitized 
      serum were rejected with time in the recipients. Furthermore, the mice died of 
      marrow graft rejection by transfer of sensitized serum prior to transplantation. 
      CONCLUSION: Taken together, our results indicated that sensitized serum played a 
      critical role in graft rejection during hematopoietic stem cell transplantation.
FAU - Xu, Lu-Hong
AU  - Xu LH
AD  - Sun Yat-sen University, Sun Yat-sen Memorial Hospital, Department of Pediatrics, 
      Guangzhou, China. E-ma-il: xulvhong@126.com.
FAU - Fang, Jian-Pei
AU  - Fang JP
FAU - Weng, Wen-Jun
AU  - Weng WJ
FAU - Xu, Hong-Gui
AU  - Xu HG
LA  - eng
PT  - Journal Article
TT  - Sensitize Serumun Allojeneik Kok Hucrelerin Rejeksiyonunda Kritik Rolu.
PL  - Turkey
TA  - Turk J Haematol
JT  - Turkish journal of haematology : official journal of Turkish Society of 
      Haematology
JID - 9606065
PMC - PMC4287027
EDAT- 2014/10/21 06:00
MHDA- 2014/10/21 06:01
PMCR- 2014/09/01
CRDT- 2014/10/21 06:00
PHST- 2014/10/21 06:00 [entrez]
PHST- 2014/10/21 06:00 [pubmed]
PHST- 2014/10/21 06:01 [medline]
PHST- 2014/09/01 00:00 [pmc-release]
AID - 1272 [pii]
AID - 10.4274/tjh.2012.0213 [doi]
PST - ppublish
SO  - Turk J Haematol. 2014 Sep 5;31(3):266-71. doi: 10.4274/tjh.2012.0213.