PMID- 25330663
OWN - NLM
STAT- MEDLINE
DCOM- 20150115
LR  - 20171116
IS  - 1000-6834 (Print)
IS  - 1000-6834 (Linking)
VI  - 30
IP  - 4
DP  - 2014 Jul
TI  - [Effect of advanced glycosylation end products on oxidative stress and MCP-1 in 
      human renal mesangial cells].
PG  - 306-10, 313
AB  - OBJECTIVE: To investigate the effects of advanced glycosylation end products 
      (AGEs) modified bovine serum albumin (AGE-BSA) on the expression of reactive 
      oxygen species (ROS) and monocyte chemoattractant protein-1 (MCP-1) in human 
      renal mesangial cells (HRMCs). METHODS: HRMCs were cultured in vitro with medium 
      containing different doses of AGE-BSA or BSA (50,100, 200, 400 mg/L) for 48 
      hours, or with AGE-BSA (200 mg/L) for different times (12, 24, 48, 72 h). 
      Immunocytochemistry assay was used to estimate the protein level of RAGE. The ROS 
      in cells were measured by flow cytometry and the mRNA expression of MCP-1 were 
      analyzed by semi-quantiative reverse transcription-polymerase chain reaction 
      (RT-PCR) after treatment with AGE-BSA or BSA. RESULTS: The protein level of RAGE 
      was upregulated in the HRMCs with AGE-BSA. The expression of ROS and MCP-1 
      significantly enhanced by incubation of AGE-BSA in a time- and dose-dependent 
      manner. The effects of AGE-BSA-induced up-regulation of ROS and MCP-1 level was 
      significantly blocked by neutralizing antibodies to RAGE, while the expression of 
      ROS and MCP-1 stood nearly unchanged after cultured with huamn IgG. CONCLUSION: 
      The expression of ROS and MCP-1 in HRMCs is induced by AGE-BSA through RAGE, 
      which may have potential effects in the pathgenic mechanism of diabetic 
      nephropathy.
FAU - Feng, Min
AU  - Feng M
FAU - Xu, Cheng-Bo
AU  - Xu CB
FAU - Wen, Jun-Ping
AU  - Wen JP
FAU - Lin, Gui-Fang
AU  - Lin GF
FAU - Lv, Qi
AU  - Lv Q
FAU - Huang, Guo-Liang
AU  - Huang GL
LA  - chi
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhongguo Ying Yong Sheng Li Xue Za Zhi
JT  - Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = 
      Chinese journal of applied physiology
JID - 9426407
RN  - 0 (CCL2 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Glycation End Products, Advanced)
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (Receptor for Advanced Glycation End Products)
RN  - 0 (Receptors, Immunologic)
RN  - 0 (advanced glycation end products-bovine serum albumin)
RN  - 27432CM55Q (Serum Albumin, Bovine)
SB  - IM
MH  - Cells, Cultured
MH  - Chemokine CCL2/*metabolism
MH  - Glycation End Products, Advanced/*pharmacology
MH  - Humans
MH  - Mesangial Cells/drug effects/*metabolism
MH  - Oxidative Stress/*drug effects
MH  - Reactive Oxygen Species/*metabolism
MH  - Receptor for Advanced Glycation End Products
MH  - Receptors, Immunologic/metabolism
MH  - Serum Albumin, Bovine/*pharmacology
EDAT- 2014/10/22 06:00
MHDA- 2015/01/16 06:00
CRDT- 2014/10/22 06:00
PHST- 2014/10/22 06:00 [entrez]
PHST- 2014/10/22 06:00 [pubmed]
PHST- 2015/01/16 06:00 [medline]
PST - ppublish
SO  - Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2014 Jul;30(4):306-10, 313.