PMID- 25333227
OWN - NLM
STAT- MEDLINE
DCOM- 20150928
LR  - 20171116
IS  - 1791-2431 (Electronic)
IS  - 1021-335X (Linking)
VI  - 33
IP  - 1
DP  - 2015 Jan
TI  - Functionally distinct subsets of CD4(+) regulatory T cells in patients with 
      laryngeal squamous cell carcinoma are indicative of immune deregulation and 
      disease progression.
PG  - 354-62
LID - 10.3892/or.2014.3553 [doi]
AB  - CD4+ regulatory T cells (Tregs) mediate immune tolerance in laryngeal squamous 
      cell carcinoma (LSCC). However, Tregs are functionally heterogeneous. Recently, 
      we reported that three distinct Treg subsets (resting Tregs, activated Tregs and 
      cytokine-secreting CD45RA-Foxp3lowCD4+ T cells) vary in the peripheral 
      circulation of patients with head and neck squamous cell carcinoma (HNSCC); 
      however, the potential implication of these Treg subsets in LSCC immunity is 
      unclear. Here, we report that activated Tregs and cytokine‑secreting 
      CD45RA-Foxp3lowCD4+ T cells were increased in LSCC patients compared with healthy 
      donors (HD) (p<0.001, p<0.001), whereas resting Tregs were decreased (p<0.001). 
      Activated Tregs inhibited the proliferation of CD4+CD25- T cells (p<0.001) and 
      secreted lower levels of interleukin-2 (p<0.001), interferon-gamma (p<0.001) and 
      tumor necrosis factor-alpha (p<0.001) compared with the cytokine-secreting 
      CD45RA-Foxp3lowCD4+ T cells. Importantly, activated Treg prevalence was 
      correlated with tumor stage (p=0.001) and nodal status (p=0.007). The prevalence 
      of naive CD4+ (p<0.001), naive CD8+ (p=0.002), and Th1 T-cell subsets (p<0.001, 
      p<0.001) was decreased in the LSCC patients. In conclusion, our findings showed 
      that activated Tregs with suppressive activity are a distinct subset of Tregs in 
      LSCC, and correlate with disease progression. Several immune system abnormalities 
      in LSCC patients are represented by expansion of functionally activated Tregs, 
      both in the circulation and tumor microenvironment along with decreased 
      frequencies of naive T-cell populations and Th1-cell populations.
FAU - Sun, Wei
AU  - Sun W
AD  - Department of Otorhinolaryngology Head and Neck Surgery, The First Affiliated 
      Hospital of Sun Yat-Sen University, Guangzhou, P.R. China.
FAU - Li, Wei-Jin
AU  - Li WJ
AD  - Department of Otorhinolaryngology Head and Neck Surgery, The First Affiliated 
      Hospital of Sun Yat-Sen University, Guangzhou, P.R. China.
FAU - Fu, Qing-Ling
AU  - Fu QL
AD  - Department of Otorhinolaryngology Head and Neck Surgery, The First Affiliated 
      Hospital of Sun Yat-Sen University, Guangzhou, P.R. China.
FAU - Wu, Chang-You
AU  - Wu CY
AD  - Institute of Immunology, Zhongshan School of Medicine, Sun Yat-Sen University, 
      Guangzhou, P.R. China.
FAU - Lin, Ji-Zhen
AU  - Lin JZ
AD  - Department of Otorhinolaryngology Head and Neck Surgery, University of Minnesota, 
      Minneapolis, MN, USA.
FAU - Zhu, Xiao-Lin
AU  - Zhu XL
AD  - Department of Otorhinolaryngology Head and Neck Surgery, The First Affiliated 
      Hospital of Sun Yat-Sen University, Guangzhou, P.R. China.
FAU - Hou, Wei-Jian
AU  - Hou WJ
AD  - Department of Otorhinolaryngology Head and Neck Surgery, The First Affiliated 
      Hospital of Sun Yat-Sen University, Guangzhou, P.R. China.
FAU - Wei, Yi
AU  - Wei Y
AD  - Department of Otorhinolaryngology Head and Neck Surgery, The First Affiliated 
      Hospital of Sun Yat-Sen University, Guangzhou, P.R. China.
FAU - Wen, Yi-Hui
AU  - Wen YH
AD  - Department of Otorhinolaryngology Head and Neck Surgery, The First Affiliated 
      Hospital of Sun Yat-Sen University, Guangzhou, P.R. China.
FAU - Wang, Yue-Jian
AU  - Wang YJ
AD  - Department of Otorhinolaryngology Head and Neck Surgery, The First Hospital of 
      Foshan, Foshan, Guangdong, P.R. China.
FAU - Wen, Wei-Ping
AU  - Wen WP
AD  - Department of Otorhinolaryngology Head and Neck Surgery, The First Affiliated 
      Hospital of Sun Yat-Sen University, Guangzhou, P.R. China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20141020
PL  - Greece
TA  - Oncol Rep
JT  - Oncology reports
JID - 9422756
RN  - 0 (FOXP3 protein, human)
RN  - 0 (Forkhead Transcription Factors)
RN  - 0 (IL2RA protein, human)
RN  - 0 (Interleukin-2 Receptor alpha Subunit)
RN  - EC 3.1.3.48 (Leukocyte Common Antigens)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - CD4-Positive T-Lymphocytes/immunology
MH  - CD8-Positive T-Lymphocytes/immunology
MH  - Case-Control Studies
MH  - Disease Progression
MH  - Female
MH  - Forkhead Transcription Factors/metabolism
MH  - Humans
MH  - Interleukin-2 Receptor alpha Subunit/metabolism
MH  - Laryngeal Neoplasms/*immunology/pathology
MH  - Leukocyte Common Antigens/metabolism
MH  - Lymphocyte Subsets/immunology
MH  - Male
MH  - Middle Aged
MH  - Neoplasms, Squamous Cell/*immunology/pathology
MH  - Reference Values
MH  - T-Lymphocytes, Regulatory/*immunology/metabolism
MH  - Th1 Cells/immunology
EDAT- 2014/10/22 06:00
MHDA- 2015/09/29 06:00
CRDT- 2014/10/22 06:00
PHST- 2014/06/26 00:00 [received]
PHST- 2014/10/07 00:00 [accepted]
PHST- 2014/10/22 06:00 [entrez]
PHST- 2014/10/22 06:00 [pubmed]
PHST- 2015/09/29 06:00 [medline]
AID - 10.3892/or.2014.3553 [doi]
PST - ppublish
SO  - Oncol Rep. 2015 Jan;33(1):354-62. doi: 10.3892/or.2014.3553. Epub 2014 Oct 20.