PMID- 25335455
OWN - NLM
STAT- MEDLINE
DCOM- 20151204
LR  - 20181202
IS  - 2192-2659 (Electronic)
IS  - 2192-2640 (Linking)
VI  - 4
IP  - 4
DP  - 2015 Mar 11
TI  - Flavonoid-modified surfaces: multifunctional bioactive biomaterials with 
      osteopromotive, anti-inflammatory, and anti-fibrotic potential.
PG  - 540-9
LID - 10.1002/adhm.201400587 [doi]
AB  - Flavonoids are small polyphenolic molecules of natural origin with antioxidant, 
      anti-inflammatory, and antibacterial properties. Here, a bioactive surface based 
      on the covalent immobilization of flavonoids taxifolin and quercitrin on titanium 
      substrates is presented, using (3-aminopropyl)triethoxysilane (APTES) as coupling 
      agent. FTIR and XPS measurements confirm the grafting of the flavonoids to the 
      surfaces. Using 2-aminoethyl diphenylborinate (DPBA, a flavonoid-specific dye), 
      the modified surfaces are imaged by fluorescence microscopy. The bioactivity of 
      the flavonoid-modified surfaces is evaluated in vitro with human umbilical cord 
      derived mesenchymal stem cells (hUC-MSCs) and human gingival fibroblasts (HGFs) 
      and compared to that of simple flavonoid coatings prepared by drop casting. 
      Flavonoid-modified surfaces show anti-inflammatory and anti-fibrotic potential on 
      HGF. In addition, Ti surfaces covalently functionalized with flavonoids promote 
      the differentiation of hUC-MSCs to osteoblasts--enhancing the expression of 
      osteogenic markers, increasing alkaline phosphatase activity and calcium 
      deposition; while drop-casted surfaces do not. These findings could have a high 
      impact in the development of advanced implantable medical devices like bone 
      implants. Given the broad range of bioactivities of flavonoid compounds, these 
      surfaces are ready to be explored for other biomedical applications, e.g., as 
      stent surface or tumor-targeted functionalized nanoparticles for cardiovascular 
      or cancer therapies.
CI  - (c) 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
FAU - Cordoba, Alba
AU  - Cordoba A
AD  - Group of Cell Therapy and Tissue Engineering, Research Institute on Health 
      Sciences (IUNICS), University of the Balearic Islands, Ctra. Valldemossa km 7.5, 
      07122, Palma de Mallorca, Spain.
FAU - Satue, Maria
AU  - Satue M
FAU - Gomez-Florit, Manuel
AU  - Gomez-Florit M
FAU - Hierro-Oliva, Margarita
AU  - Hierro-Oliva M
FAU - Petzold, Christiane
AU  - Petzold C
FAU - Lyngstadaas, Staale P
AU  - Lyngstadaas SP
FAU - Gonzalez-Martin, Maria Luisa
AU  - Gonzalez-Martin ML
FAU - Monjo, Marta
AU  - Monjo M
FAU - Ramis, Joana M
AU  - Ramis JM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20141021
PL  - Germany
TA  - Adv Healthc Mater
JT  - Advanced healthcare materials
JID - 101581613
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Biocompatible Materials)
RN  - 0 (Flavonoids)
RN  - D1JT611TNE (Titanium)
RN  - EC 1.1.1.27 (L-Lactate Dehydrogenase)
RN  - EC 3.1.3.1 (Alkaline Phosphatase)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Alkaline Phosphatase/metabolism
MH  - Anti-Inflammatory Agents/*pharmacology
MH  - Biocompatible Materials/*pharmacology
MH  - Calcium/metabolism
MH  - Cell Death/drug effects
MH  - Cell Shape/drug effects
MH  - Cells, Cultured
MH  - Fibroblasts/cytology
MH  - Fibrosis
MH  - Flavonoids/chemistry/*pharmacology
MH  - Gene Expression Regulation/drug effects
MH  - Gingiva/cytology
MH  - Humans
MH  - L-Lactate Dehydrogenase/metabolism
MH  - Mesenchymal Stem Cells/cytology/drug effects/metabolism
MH  - Microscopy, Fluorescence
MH  - Osteogenesis/*drug effects
MH  - Photoelectron Spectroscopy
MH  - Spectroscopy, Fourier Transform Infrared
MH  - Titanium/pharmacology
MH  - Wettability
OTO - NOTNLM
OT  - bioactivity
OT  - biomaterials
OT  - flavonoids
OT  - surface functionalization
OT  - titanium
EDAT- 2014/10/23 06:00
MHDA- 2015/12/15 06:00
CRDT- 2014/10/23 06:00
PHST- 2014/09/23 00:00 [received]
PHST- 2014/10/05 00:00 [revised]
PHST- 2014/10/23 06:00 [entrez]
PHST- 2014/10/23 06:00 [pubmed]
PHST- 2015/12/15 06:00 [medline]
AID - 10.1002/adhm.201400587 [doi]
PST - ppublish
SO  - Adv Healthc Mater. 2015 Mar 11;4(4):540-9. doi: 10.1002/adhm.201400587. Epub 2014 
      Oct 21.