PMID- 25337115
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20141022
LR  - 20211021
IS  - 1673-5374 (Print)
IS  - 1876-7958 (Electronic)
IS  - 1673-5374 (Linking)
VI  - 7
IP  - 34
DP  - 2012 Dec 5
TI  - Neural cell injury microenvironment induces neural differentiation of human 
      umbilical cord mesenchymal stem cells.
PG  - 2689-97
LID - 10.3969/j.issn.1673-5374.2012.34.006 [doi]
AB  - This study aimed to investigate the neural differentiation of human umbilical 
      cord mesenchymal stem cells (hUCMSCs) under the induction of injured neural 
      cells. After in vitro isolation and culture, passage 5 hUCMSCs were used for 
      experimentation. hUCMSCs were co-cultured with normal or Abeta1-40-injured PC12 
      cells, PC12 cell supernatant or PC12 cell lysate in a Transwell co-culture 
      system. Western blot analysis and flow cytometry results showed that choline 
      acetyltransferase and microtubule-associated protein 2, a specific marker for 
      neural cells, were expressed in hUCMSCs under various culture conditions, and 
      highest expression was observed in the hUCMSCs co-cultured with injured PC12 
      cells. Choline acetyltransferase and microtubule-associated protein 2 were not 
      expressed in hUCMSCs cultured alone (no treatment). Cell Counting Kit-8 assay 
      results showed that hUCMSCs under co-culture conditions promoted the 
      proliferation of injured PC12 cells. These findings suggest that the 
      microenvironment during neural tissue injury can effectively induce neural cell 
      differentiation of hUCMSCs. These differentiated hUCMSCs likely accelerate the 
      repair of injured neural cells.
FAU - Zhou, Jin
AU  - Zhou J
AD  - Department of Neurology, First People's Hospital of Shenyang, Shenyang 110041, 
      Liaoning Province, China.
FAU - Tian, Guoping
AU  - Tian G
AD  - Department of Neurology, First People's Hospital of Shenyang, Shenyang 110041, 
      Liaoning Province, China.
FAU - Wang, Jinge
AU  - Wang J
AD  - Department of Neurology, First People's Hospital of Shenyang, Shenyang 110041, 
      Liaoning Province, China.
FAU - Luo, Xiaoguang
AU  - Luo X
AD  - First Affiliated Hospital of China Medical University, Shenyang 110001, Liaoning 
      Province, China.
FAU - Zhang, Siyang
AU  - Zhang S
AD  - College of Basic Medical Sciences, China Medical University, Shenyang 110001, 
      Liaoning Province, China.
FAU - Li, Jianping
AU  - Li J
AD  - Liaoning Provincial Blood Center, Shenyang 110044, Liaoning Province, China.
FAU - Li, Li
AU  - Li L
AD  - Department of Neurology, First People's Hospital of Shenyang, Shenyang 110041, 
      Liaoning Province, China.
FAU - Xu, Bing
AU  - Xu B
AD  - Department of Neurology, First People's Hospital of Shenyang, Shenyang 110041, 
      Liaoning Province, China.
FAU - Zhu, Feng
AU  - Zhu F
AD  - Department of Neurology, First People's Hospital of Shenyang, Shenyang 110041, 
      Liaoning Province, China.
FAU - Wang, Xia
AU  - Wang X
AD  - Department of Neurology, First People's Hospital of Shenyang, Shenyang 110041, 
      Liaoning Province, China.
FAU - Jia, Chunhong
AU  - Jia C
AD  - Department of Neurology, First People's Hospital of Shenyang, Shenyang 110041, 
      Liaoning Province, China.
FAU - Zhao, Weijin
AU  - Zhao W
AD  - Department of Neurology, First People's Hospital of Shenyang, Shenyang 110041, 
      Liaoning Province, China.
FAU - Zhao, Danyang
AU  - Zhao D
AD  - Department of Neurology, First People's Hospital of Shenyang, Shenyang 110041, 
      Liaoning Province, China.
FAU - Xu, Aihua
AU  - Xu A
AD  - Department of Neurology, First People's Hospital of Shenyang, Shenyang 110041, 
      Liaoning Province, China.
LA  - eng
PT  - Journal Article
PL  - India
TA  - Neural Regen Res
JT  - Neural regeneration research
JID - 101316351
PMC - PMC4200737
OTO - NOTNLM
OT  - Transwell
OT  - co-culture
OT  - differentiation
OT  - induction
OT  - injured cell
OT  - microtubule-associated protein 2
OT  - neural cell
OT  - neural regeneration
OT  - regeneration
OT  - stem cell
OT  - umbilical cord mesenchymal stem cell
COIS- Conflicts of interest: None declared.
EDAT- 2012/12/05 00:00
MHDA- 2012/12/05 00:01
PMCR- 2012/12/05
CRDT- 2014/10/23 06:00
PHST- 2012/09/11 00:00 [received]
PHST- 2012/11/23 00:00 [accepted]
PHST- 2014/10/23 06:00 [entrez]
PHST- 2012/12/05 00:00 [pubmed]
PHST- 2012/12/05 00:01 [medline]
PHST- 2012/12/05 00:00 [pmc-release]
AID - NRR-7-2689 [pii]
AID - 10.3969/j.issn.1673-5374.2012.34.006 [doi]
PST - ppublish
SO  - Neural Regen Res. 2012 Dec 5;7(34):2689-97. doi: 
      10.3969/j.issn.1673-5374.2012.34.006.