PMID- 25337257 OWN - NLM STAT- MEDLINE DCOM- 20150702 LR - 20221207 IS - 1936-2625 (Electronic) IS - 1936-2625 (Linking) VI - 7 IP - 9 DP - 2014 TI - Angioimmunoblastic T-cell lymphoma in Taiwan shows a frequent gain of ITK gene. PG - 6097-107 AB - Angioimmunoblastic T-cell lymphoma (AITL) is an aggressive peripheral T-cell lymphoma (PTCL) of follicular helper T-cell origin and is rare in Taiwan. There are overlapping features of AITL and peripheral T-cell lymphoma with a follicular growth pattern (PTCL-F). Around one fifth of PTCL-F exhibits t(5;9)(q33;q22)/ITK-SYK chromosomal translocation, which is essentially absent in AITL. We retrospectively investigated 35 cases of AITL from Taiwan with histopathology review, immunohistochemistry, in situ hybridization for Epstein-Barr virus (EBV) and fluorescence in situ hybridization (FISH) for t(5;9)(q33;q22)/ITK-SYK and correlated the results with overall survival. Twenty-six cases of not otherwise specified PTCL (PTCL-NOS) were also examined by FISH for comparison. Most AITL patients were male (69%) and elderly (median age at 67 years) with frequent bone marrow involvement (53%), high Ann Arbor stages (77%), and elevated serum lactate dehydrogenase (68%). Most cases (80%) showed a typical CD4+/CD8- phenotype and in 90% cases there were scattered EBV-positive B-cells (less than 10% cells). None of these cases showed t(5;9)(q33;q22)/ITK-SYK translocation by FISH. Gain of ITK and SYK gene was identified in 38% and 14% tumors, respectively, but both were not associated with overall survival. Performance status < 2 was associated with a better outcome but not the other clinicopathological factors. All PTCL-NOS cases were negative for ITK-SYK translocation with similar rates (38% and 12%, respectively) of gains at ITK and SYK loci as that of AITL. In this so far the largest series of AITL from Taiwan, we reported the clinicopathological features and FISH findings on ITK and SYK genes. We confirmed the absence of t(5;9)(q33;q22)/ITK-SYK translocation, which may serve as an additional differential diagnostic tool from PTCL-F when present. PTCL-NOS shared a similar pattern of ITK and SYK gains with AITL. More studies are warranted to elucidate the roles of SYK and ITK and other genes in the lymphomagenesis of AITL in Taiwan. FAU - Liang, Peir-In AU - Liang PI AD - Department of Pathology, Kaohsiung Medical University Hospital, Kaohsiung Medical University Kaohsiung, Taiwan. FAU - Chang, Sheng-Tsung AU - Chang ST AD - Department of Pathology, Chi-Mei Medical Center and Department of Nursing, National Tainan Institute of Nursing Tainan, Taiwan. FAU - Lin, Ming-Yen AU - Lin MY AD - Division of Nephrology, Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung Medical University and Faculty of Renal Care, College of Medicine, Kaohsiung Medical University Kaohsiung, Taiwan. FAU - Hsieh, Yen-Chuan AU - Hsieh YC AD - Department of Pathology, Chi-Mei Medical Center and Department of Medical Laboratory Science and Biotechnology, Chung Hwa University of Medical Technology Tainan, Taiwan. FAU - Chu, Pei-Yi AU - Chu PY AD - Department of Pathology, St. Martin De Porres Hospital, Chiayi and School of Medicine, Fu-Jen Catholic University New Taipei City, Taiwan. FAU - Chen, Chih-Jung AU - Chen CJ AD - Department of Surgical Pathology, Changhua Christian Hospital, Changhua, School of Medicine, Chung Shan Medical University Taichung, Taiwan ; Department of Medical Technology, Jen-Teh Junior College of Medicine, Nursing and Management Miaoli, Taiwan. FAU - Lin, Kai-Jen AU - Lin KJ AD - Department of Pathology, E-Da Hospital/I-Shou University Kaohsiung, Taiwan. FAU - Jung, Yun-Chih AU - Jung YC AD - Department of Pathology, Sin-Lau Christian Hospital Tainan, Taiwan. FAU - Hwang, Wei-Shou AU - Hwang WS AD - Division of Hematology and Oncology, Department of Internal Medicine, Chung-Shan Medical University Hospital and School of Medicine, Chung-Shan Medical University Taichung, Taiwan. FAU - Huang, Wen-Tsung AU - Huang WT AD - Division of Hemato-Oncology, Department of Internal Medicine, Chi-Mei Medical Center Liouying, Tainan, Taiwan. FAU - Chang, Wei-Chin AU - Chang WC AD - Department of Pathology, Mackay Medical College and Mackay Memorial Hospital, Mackay Junior College of Medicine, Nursing, and Management, New Taipei City, and Institute of Clinical Medicine, National Yang-Ming University Taipei, Taiwan. FAU - Ye, Hongtao AU - Ye H AD - Department of Histopathology, Royal National Orthopaedic Hospital NHS, Middlesex, United Kingdom and Cancer Hospital of Guangxi Medical University Nanning, P. R. China. FAU - Chuang, Shih-Sung AU - Chuang SS AD - Department of Pathology, Chi-Mei Medical Center, Tainan, Taipei Medical University and National Taiwan University Taipei, Taiwan. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20140815 PL - United States TA - Int J Clin Exp Pathol JT - International journal of clinical and experimental pathology JID - 101480565 RN - 0 (Intracellular Signaling Peptides and Proteins) RN - EC 2.7.10.1 (Protein-Tyrosine Kinases) RN - EC 2.7.10.2 (SYK protein, human) RN - EC 2.7.10.2 (Syk Kinase) RN - EC 2.7.10.2 (emt protein-tyrosine kinase) SB - IM MH - Aged MH - Aged, 80 and over MH - Asian People/genetics MH - Chromosomes, Human, Pair 5 MH - Chromosomes, Human, Pair 9 MH - Female MH - Gene Amplification/*genetics MH - Genetic Predisposition to Disease MH - Herpesvirus 4, Human/isolation & purification MH - Humans MH - Immunoblastic Lymphadenopathy/enzymology/ethnology/*genetics/pathology/virology MH - Immunohistochemistry MH - In Situ Hybridization, Fluorescence MH - Intracellular Signaling Peptides and Proteins/genetics MH - Lymphoma, T-Cell/enzymology/ethnology/*genetics/pathology/virology MH - Male MH - Middle Aged MH - Phenotype MH - Predictive Value of Tests MH - Prognosis MH - Protein-Tyrosine Kinases/*genetics MH - Retrospective Studies MH - Syk Kinase MH - Taiwan/epidemiology MH - Time Factors MH - Translocation, Genetic PMC - PMC4203228 OTO - NOTNLM OT - Angioimmunoblastic T-cell lymphoma OT - ITK OT - SYK OT - Taiwan OT - follicular T-cell lymphoma OT - follicular helper T-cell OT - peripheral T-cell lymphoma EDAT- 2014/10/23 06:00 MHDA- 2015/07/03 06:00 PMCR- 2014/08/15 CRDT- 2014/10/23 06:00 PHST- 2014/07/09 00:00 [received] PHST- 2014/08/21 00:00 [accepted] PHST- 2014/10/23 06:00 [entrez] PHST- 2014/10/23 06:00 [pubmed] PHST- 2015/07/03 06:00 [medline] PHST- 2014/08/15 00:00 [pmc-release] PST - epublish SO - Int J Clin Exp Pathol. 2014 Aug 15;7(9):6097-107. eCollection 2014.