PMID- 25337716
OWN - NLM
STAT- MEDLINE
DCOM- 20150706
LR  - 20211021
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 9
IP  - 10
DP  - 2014
TI  - Objective determination of optimal number of spectral-domain optical coherence 
      tomographic images of retina to average.
PG  - e110550
LID - 10.1371/journal.pone.0110550 [doi]
LID - e110550
AB  - PURPOSE: To determine by objective methods the minimum number of spectral-domain 
      optical coherence tomographic (SD-OCT) images to average to obtain the clearest 
      retinal image. METHODS: SD-OCT Images were obtained from 9 healthy eyes and also 
      from a phantom eye model. The SD-OCT images were obtained by averaging 1, 5, 20, 
      60, and 100 B-scan images. The reflectivity (mean gray value) of the different 
      retinal layers was evaluated in these images. The image quality was evaluated by 
      the size of the standard deviations (SDs) and the contrast-to-noise ratios 
      (CNRs). A phantom eye model made by TiO2 silicone plates was also examined. 
      RESULTS: The SDs decreased significantly when the number of images averaged 
      increased from 1 to 5 and also from 5 to 20 (P<0.05, post hoc Tukey's honestly 
      significant difference tests). The SD of the automatic real time averaging of 1 
      (ART = 1) and ART = 5 were significantly larger than the SD of ART = 100 
      (P<0.05). The SDs of all other averaged numbers were not significantly larger 
      than that of ART = 100. The CNR increased with an increase in the number of 
      images averaged, and there was a significant increase between ART = 1 to 5 and 
      between ART = 5 to 20 (P<0.05). No significant differences in the CNR was 
      observed between ART = 5, ART = 20 and ART = 60. Similar results were obtained 
      with the phantom eye model. CONCLUSIONS: Although the image quality of the SD-OCT 
      images of the retina improved with an increase in the number of images averaged, 
      it does not improve significantly by averaging more than 20 images.
FAU - Shirasawa, Makoto
AU  - Shirasawa M
AD  - Department of Ophthalmology, Kagoshima University Graduate School of Medical and 
      Dental Sciences, Kagoshima, Japan.
FAU - Sakamoto, Taiji
AU  - Sakamoto T
AD  - Department of Ophthalmology, Kagoshima University Graduate School of Medical and 
      Dental Sciences, Kagoshima, Japan.
FAU - Terasaki, Hiroto
AU  - Terasaki H
AD  - Department of Ophthalmology, Kagoshima University Graduate School of Medical and 
      Dental Sciences, Kagoshima, Japan.
FAU - Yamashita, Takehiro
AU  - Yamashita T
AD  - Department of Ophthalmology, Kagoshima University Graduate School of Medical and 
      Dental Sciences, Kagoshima, Japan.
FAU - Uchino, Eisuke
AU  - Uchino E
AD  - Department of Ophthalmology, Kagoshima University Graduate School of Medical and 
      Dental Sciences, Kagoshima, Japan.
FAU - Sonoda, Shozo
AU  - Sonoda S
AD  - Department of Ophthalmology, Kagoshima University Graduate School of Medical and 
      Dental Sciences, Kagoshima, Japan.
LA  - eng
PT  - Journal Article
PT  - Observational Study
PT  - Research Support, Non-U.S. Gov't
DEP - 20141022
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
SB  - IM
MH  - Adult
MH  - Cross-Sectional Studies
MH  - Female
MH  - Humans
MH  - Male
MH  - Phantoms, Imaging
MH  - Prospective Studies
MH  - Reference Values
MH  - Retina/*anatomy & histology
MH  - Signal-To-Noise Ratio
MH  - *Tomography, Optical Coherence
MH  - Young Adult
PMC - PMC4206300
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2014/10/23 06:00
MHDA- 2015/07/07 06:00
PMCR- 2014/10/22
CRDT- 2014/10/23 06:00
PHST- 2014/05/26 00:00 [received]
PHST- 2014/09/15 00:00 [accepted]
PHST- 2014/10/23 06:00 [entrez]
PHST- 2014/10/23 06:00 [pubmed]
PHST- 2015/07/07 06:00 [medline]
PHST- 2014/10/22 00:00 [pmc-release]
AID - PONE-D-14-23399 [pii]
AID - 10.1371/journal.pone.0110550 [doi]
PST - epublish
SO  - PLoS One. 2014 Oct 22;9(10):e110550. doi: 10.1371/journal.pone.0110550. 
      eCollection 2014.