PMID- 25343978 OWN - NLM STAT- MEDLINE DCOM- 20160201 LR - 20150228 IS - 1432-0614 (Electronic) IS - 0175-7598 (Linking) VI - 99 IP - 6 DP - 2015 Mar TI - Using cassava distiller's dried grains as carbon and microbe sources to enhance denitrification of nitrate-contaminated groundwater. PG - 2839-47 LID - 10.1007/s00253-014-6155-z [doi] AB - Nitrate removal from synthetic and real groundwater was investigated by using cassava distiller's dried grains (CDDG), which served as sole carbon source as well as the only microbe seed. It was found that remarkably higher total nitrogen removal efficiency (96.8 +/- 0.6 %) could be reached; the accumulation of nitrite and the releases of organic compounds, meanwhile, were insignificant in the denitrification process. Scanning electron microscope (SEM) analysis showed that CDDG were degraded during the denitrification process. Further investigation showed that CDDG were anaerobically hydrolyzed and acidified to butyric acid, acetic acid, and carbohydrate, which could be utilized directly as the reducing equivalent providers for denitrification by the microorganisms separated from CDDG. Microbial community analysis revealed that the fungi and bacteria present in the original CDDG functioned as the denitrifiers, which mainly consisted of Aspergillus (69.8 %) and Rhizomucor (15.9 %) in the fungi community and Burkholderia (20.6 %) and Rhizobium (15.9 %) in the bacteria community, respectively. Finally, the use of CDDG as both carbon and microbial sources for real groundwater denitrification was testified to be feasible and safe with a total nitrogen removal efficiency of around 100 %. FAU - Wan, Rui AU - Wan R AD - State Key Laboratory of Pollution Control and Resources Reuse, School of Environmental Science and Engineering, Tongji University, 1239 Siping Road, Shanghai, 200092, China. FAU - Zheng, Xiong AU - Zheng X FAU - Chen, Yinguang AU - Chen Y FAU - Wang, Huaichen AU - Wang H LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20141026 PL - Germany TA - Appl Microbiol Biotechnol JT - Applied microbiology and biotechnology JID - 8406612 RN - 0 (Nitrates) RN - 0 (Nitrites) RN - 0 (Water Pollutants, Chemical) RN - 7440-44-0 (Carbon) SB - IM MH - Aspergillus/metabolism MH - Bioreactors/microbiology MH - Burkholderia/metabolism MH - Carbon/*chemistry MH - *Denitrification MH - Groundwater/*chemistry MH - Manihot/*chemistry/*microbiology MH - Nitrates/*isolation & purification MH - Nitrites/chemistry MH - Rhizobium/metabolism MH - Rhizomucor/metabolism MH - Water Pollutants, Chemical/*isolation & purification MH - Water Purification/methods EDAT- 2014/10/26 06:00 MHDA- 2016/02/02 06:00 CRDT- 2014/10/26 06:00 PHST- 2014/07/22 00:00 [received] PHST- 2014/10/12 00:00 [accepted] PHST- 2014/10/07 00:00 [revised] PHST- 2014/10/26 06:00 [entrez] PHST- 2014/10/26 06:00 [pubmed] PHST- 2016/02/02 06:00 [medline] AID - 10.1007/s00253-014-6155-z [doi] PST - ppublish SO - Appl Microbiol Biotechnol. 2015 Mar;99(6):2839-47. doi: 10.1007/s00253-014-6155-z. Epub 2014 Oct 26.