PMID- 25344857
OWN - NLM
STAT- MEDLINE
DCOM- 20160603
LR  - 20181113
IS  - 1432-184X (Electronic)
IS  - 0095-3628 (Linking)
VI  - 69
IP  - 3
DP  - 2015 Apr
TI  - Bacterial domination over archaea in ammonia oxidation in a monsoon-driven 
      tropical estuary.
PG  - 544-53
LID - 10.1007/s00248-014-0519-x [doi]
AB  - Autotrophic ammonia oxidizing microorganisms, which are responsible for the 
      rate-limiting step of nitrification in most aquatic systems, have not been 
      studied in tropical estuaries. Cochin estuary (CE) is one of the largest, 
      productive, and monsoon-driven estuary in India opening into the southeast 
      Arabian Sea. CE receives surplus quantities of ammonia through industrial and 
      domestic discharges. The distribution of ammonia-oxidizing bacteria (AOB), 
      ammonia-oxidizing archaea (AOA), and anaerobic ammonia-oxidizing bacteria 
      (anammox) were studied using fluorescence in situ hybridization (FISH) and their 
      relative contribution to the process as well as the governing factors were 
      examined and reported for the first time from CE. The order of occurrence of 
      these assemblages was beta-proteobacteria (0.79 to 2 x 10(5) cells 
      ml(-1)) > gamma-proteobacteria (0.9 to 4.6 x 10(4) cells ml(-1)) > anammox (0.49 to 
      1.9 x 10(4) cells ml(-1)) > AOA (0.56 to 6.3 x 10(3) cells ml(-1)). Phylogenetic 
      analysis of DGGE bands showed major affiliation of AOB to beta-proteobacteria, while 
      AOA was affiliated to Crenarchaeota. The abundance of AOB was mostly influenced 
      by ammonia concentrations. The recovered ammonia oxidation rate of AOB was in the 
      range of 45-65%, whereas for AOA, it was 15-45%, indicating that AOB were mostly 
      responsible for the ammonia oxidation in CE during the study period. Overall, the 
      present study provides an insight into the relevance and contribution of 
      different groups of ammonia oxidizing bacteria in CE and emphasizes the need for 
      further in depth studies across space and on season scale.
FAU - Puthiya Veettil, Vipindas
AU  - Puthiya Veettil V
AD  - Regional Centre, CSIR - National Institute of Oceanography, Cochin, Kerala, 
      682018, India.
FAU - Abdulaziz, Anas
AU  - Abdulaziz A
FAU - Chekidhenkuzhiyil, Jasmin
AU  - Chekidhenkuzhiyil J
FAU - Kalanthingal Ramkollath, Lallu
AU  - Kalanthingal Ramkollath L
FAU - Karayadi Hamza, Fausia
AU  - Karayadi Hamza F
FAU - Kizhakkepat Kalam, Balachandran
AU  - Kizhakkepat Kalam B
FAU - Kallungal Ravunnikutty, Muraleedharan
AU  - Kallungal Ravunnikutty M
FAU - Nair, Shanta
AU  - Nair S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20141026
PL  - United States
TA  - Microb Ecol
JT  - Microbial ecology
JID - 7500663
RN  - 0 (DNA, Bacterial)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 7664-41-7 (Ammonia)
SB  - IM
MH  - Ammonia/*metabolism
MH  - Archaea/genetics/*physiology
MH  - *Bacterial Physiological Phenomena/genetics
MH  - DNA, Bacterial/genetics/metabolism
MH  - Estuaries
MH  - India
MH  - Molecular Sequence Data
MH  - Oxidation-Reduction
MH  - RNA, Ribosomal, 16S/genetics/metabolism
MH  - Seawater/*microbiology
MH  - Sequence Analysis, DNA
MH  - Tropical Climate
EDAT- 2014/10/27 06:00
MHDA- 2016/06/04 06:00
CRDT- 2014/10/27 06:00
PHST- 2014/01/10 00:00 [received]
PHST- 2014/10/09 00:00 [accepted]
PHST- 2014/10/27 06:00 [entrez]
PHST- 2014/10/27 06:00 [pubmed]
PHST- 2016/06/04 06:00 [medline]
AID - 10.1007/s00248-014-0519-x [doi]
PST - ppublish
SO  - Microb Ecol. 2015 Apr;69(3):544-53. doi: 10.1007/s00248-014-0519-x. Epub 2014 Oct 
      26.