PMID- 2534580
OWN - NLM
STAT- MEDLINE
DCOM- 19900627
LR  - 20131121
IS  - 1000-503X (Print)
IS  - 1000-503X (Linking)
VI  - 11
IP  - 6
DP  - 1989 Dec
TI  - [Biotinylated Y chromosome specific probe for human sexing].
PG  - 433-7
AB  - Human chromosome DNA from WBC or fetus chorion samples were digested with Hae III 
      and hybridized with biotinylated Y chromosome specific probe by Southern 
      blotting, and hybridization signals were developed by the ABC 
      (Avidin-biotin-alkaline phosphatase complex) system. The hybridization signal for 
      0.1 microgram of male DNA could be detected clearly, while the signal for even 5 
      micrograms of female DNA could not. Parallel tests showed that the sexing results 
      using 32P-labeled and biotinylated Y probe were identical. This suggests that the 
      biotinylated Y probe can be applied to the determination of X-linked genetic 
      diseases and sex abnormality, forensic analysis, sex determination of sportsmen 
      and women, heterosexual transplantation of bone marrow, etc. It could become a 
      convenient means for genetic diagnosis.
FAU - Wang, S W
AU  - Wang SW
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhongguo Yi Xue Ke Xue Yuan Xue Bao
JT  - Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae
JID - 8006230
RN  - 0 (Affinity Labels)
RN  - 0 (Azides)
RN  - 0 (DNA Probes)
RN  - 6SO6U10H04 (Biotin)
RN  - 96087-37-5 (photobiotin)
SB  - IM
MH  - Affinity Labels
MH  - Azides
MH  - Biotin/analogs & derivatives
MH  - *DNA Probes
MH  - Humans
MH  - *Sex Determination Analysis
MH  - *Y Chromosome
EDAT- 1989/12/01 00:00
MHDA- 1989/12/01 00:01
CRDT- 1989/12/01 00:00
PHST- 1989/12/01 00:00 [pubmed]
PHST- 1989/12/01 00:01 [medline]
PHST- 1989/12/01 00:00 [entrez]
PST - ppublish
SO  - Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 1989 Dec;11(6):433-7.