PMID- 25346485
OWN - NLM
STAT- MEDLINE
DCOM- 20150507
LR  - 20181113
IS  - 1365-2567 (Electronic)
IS  - 0019-2805 (Print)
IS  - 0019-2805 (Linking)
VI  - 144
IP  - 4
DP  - 2015 Apr
TI  - The presence of interleukin-27 during monocyte-derived dendritic cell 
      differentiation promotes improved antigen processing and stimulation of T cells.
PG  - 649-60
LID - 10.1111/imm.12417 [doi]
AB  - Dendritic cells (DCs) are potent antigen-presenting cells necessary to establish 
      effective adaptive immune responses. The cytokine environment that exists at the 
      time of DC differentiation may be an important but often ignored determinant in 
      the phenotypic and functional properties of DCs. Interleukin-27 (IL-27) is a 
      unique cytokine that has both inflammatory and immune suppressive activities. 
      Although it can both promote and oppose activity of different T-cell subsets, 
      mostly anti-inflammatory activity has been described toward macrophages and DCs. 
      However, the specific effect of IL-27 during DC differentiation and how that may 
      change the nature of the antigen-presenting cell has not been investigated. In 
      this report, we show that IL-27 treatment during monocyte-derived DC 
      differentiation enhanced the ability to process antigens and stimulate T-cell 
      activity. DCs differentiated in the presence of IL-27 showed enhanced 
      acidification of latex bead-containing phagosomes that was consistent with 
      elevated expression of vacuolar-ATPases. This resulted in inhibition of 
      intracellular growth of Staphylococcus aureus. In addition, the levels of MHC 
      class II surface expression were higher in DCs differentiated in the presence of 
      IL-27. Production of IL-12 was also significantly increased during S. aureus 
      infection of IL-27-differentiated DCs. The net effect of these activities was 
      enhanced CD4(+) T-cell proliferation and T helper type 1 cytokine production. 
      These findings are important to a wide number of immunological contexts and 
      should be considered in the development of future vaccines.
CI  - (c) 2014 John Wiley & Sons Ltd.
FAU - Jung, Joo-Yong
AU  - Jung JY
AD  - Department of Biology, Briar Cliff University, Sioux City, IA, USA.
FAU - Roberts, Lawton L
AU  - Roberts LL
FAU - Robinson, Cory M
AU  - Robinson CM
LA  - eng
GR  - K99 HL093300/HL/NHLBI NIH HHS/United States
GR  - R00 HL093300/HL/NHLBI NIH HHS/United States
GR  - HL093300/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Immunology
JT  - Immunology
JID - 0374672
RN  - 0 (Histocompatibility Antigens Class II)
RN  - 0 (Interleukin-27)
RN  - 187348-17-0 (Interleukin-12)
SB  - IM
MH  - Antigen Presentation/*drug effects
MH  - CD4-Positive T-Lymphocytes/*drug effects/immunology/metabolism
MH  - Cell Differentiation/*drug effects
MH  - Cell Proliferation/drug effects
MH  - Cells, Cultured
MH  - Coculture Techniques
MH  - Dendritic Cells/*drug effects/immunology/metabolism/microbiology
MH  - Histocompatibility Antigens Class II/immunology/metabolism
MH  - Humans
MH  - Hydrogen-Ion Concentration
MH  - Interleukin-12/immunology/metabolism
MH  - Interleukin-27/*pharmacology
MH  - Lymphocyte Activation/*drug effects
MH  - Monocytes/*drug effects/immunology/metabolism
MH  - Phagosomes/drug effects/immunology/metabolism
MH  - Signal Transduction
MH  - Staphylococcus aureus/drug effects/growth & development/immunology
MH  - Th1 Cells/drug effects/immunology/metabolism
MH  - Time Factors
PMC - PMC4368171
OTO - NOTNLM
OT  - antigen-presenting cell
OT  - dendritic cells
OT  - interleukin-27
EDAT- 2014/10/28 06:00
MHDA- 2015/05/08 06:00
PMCR- 2016/04/01
CRDT- 2014/10/28 06:00
PHST- 2014/06/23 00:00 [received]
PHST- 2014/10/17 00:00 [revised]
PHST- 2014/10/19 00:00 [accepted]
PHST- 2014/10/28 06:00 [entrez]
PHST- 2014/10/28 06:00 [pubmed]
PHST- 2015/05/08 06:00 [medline]
PHST- 2016/04/01 00:00 [pmc-release]
AID - 10.1111/imm.12417 [doi]
PST - ppublish
SO  - Immunology. 2015 Apr;144(4):649-60. doi: 10.1111/imm.12417.