PMID- 25347402
OWN - NLM
STAT- MEDLINE
DCOM- 20151022
LR  - 20141118
IS  - 1520-6882 (Electronic)
IS  - 0003-2700 (Linking)
VI  - 86
IP  - 22
DP  - 2014 Nov 18
TI  - Assessment of serum protein dynamics by native SILAC flooding (SILflood).
PG  - 11033-7
LID - 10.1021/ac502883p [doi]
AB  - Turnover of blood serum proteins is a vital function in mammals, but technical 
      challenges have thus far prevented comprehensive measurements of serum protein 
      half-lives. Here, we injected native serum from heavy stable isotope labeled 
      (SIL) mice into nonlabeled recipients to quantify turnover of more than 200 
      proteins using mass spectrometry with high reproducibility and accuracy. We found 
      a median of 19.4 h and a total range of 6-70 h for calculated half-lives. 
      Moreover, we observed similar half-lives for proteins with equal function. To 
      demonstrate the value and effectiveness of SILflood, we investigated the impaired 
      serum clearance in beta2-microglobulin (B2M-/-) deficient mice. Notably, we found 
      that serum albumin and IgG half-lives were clearly reduced in B2M deficient 
      animals compared to control animals. Taken together, our results demonstrate that 
      SILflood is a versatile tool to investigate serum half-lives under regular and 
      pathological conditions in living animals.
FAU - Nolte, Hendrik
AU  - Nolte H
AD  - Institute for Genetics and Cologne Excellence Cluster on Cellular Stress 
      Responses in Aging-Associated Diseases (CECAD), University of Cologne , 
      Joseph-Stelzmann-Strasse 26, 50931 Cologne, Germany.
FAU - Holper, Soraya
AU  - Holper S
FAU - Selbach, Matthias
AU  - Selbach M
FAU - Braun, Thomas
AU  - Braun T
FAU - Kruger, Marcus
AU  - Kruger M
LA  - eng
PT  - Journal Article
DEP - 20141106
PL  - United States
TA  - Anal Chem
JT  - Analytical chemistry
JID - 0370536
RN  - 0 (Carbon Isotopes)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Serum Albumin)
RN  - 0 (beta 2-Microglobulin)
SB  - IM
MH  - Animals
MH  - Carbon Isotopes
MH  - Cells, Cultured
MH  - Half-Life
MH  - High-Throughput Screening Assays
MH  - Immunoglobulin G/blood
MH  - *Isotope Labeling
MH  - Male
MH  - Mass Spectrometry
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Serum/*chemistry
MH  - Serum Albumin/analysis
MH  - beta 2-Microglobulin/deficiency
EDAT- 2014/10/28 06:00
MHDA- 2015/10/23 06:00
CRDT- 2014/10/28 06:00
PHST- 2014/10/28 06:00 [entrez]
PHST- 2014/10/28 06:00 [pubmed]
PHST- 2015/10/23 06:00 [medline]
AID - 10.1021/ac502883p [doi]
PST - ppublish
SO  - Anal Chem. 2014 Nov 18;86(22):11033-7. doi: 10.1021/ac502883p. Epub 2014 Nov 6.