PMID- 25348585
OWN - NLM
STAT- MEDLINE
DCOM- 20150709
LR  - 20181202
IS  - 1098-2795 (Electronic)
IS  - 1040-452X (Linking)
VI  - 81
IP  - 11
DP  - 2014 Nov
TI  - Epidermal growth factor-network signaling mediates luteinizing hormone regulation 
      of BNP and CNP and their receptor NPR2 during porcine oocyte meiotic resumption.
PG  - 1030-41
LID - 10.1002/mrd.22424 [doi]
AB  - The epidermal growth factor (EGF) network, induced by luteinizing hormone (LH), 
      plays an essential role during the regulation of oocyte maturation, cumulus 
      expansion, and ovulation. Binding of brain natriuretic peptide (BNP) and C-type 
      natriuretic peptide (CNP) to natriuretic receptor 2 (NPR2) generates cyclic 
      guanosine monophosphate (cGMP), a key inhibitor that sustains porcine oocyte 
      meiotic arrest. This correlation suggests that LH interacts with 
      natriuretic-peptide signaling, possibly via the EGF network, to promote porcine 
      meiotic resumption. In testing this hypothesis, we found that the majority of 
      porcine oocytes remain arrested in the germinal-vesicle stage after 44 hr of 
      co-culturing cumulus-oocyte complexes with 10(7) granulosa cells, which secreted 
      active BNP and CNP; these natriuretic peptides associate with NPR2 on cumulus 
      cells, thereby inhibiting porcine oocyte maturation. This inhibitory effect of 
      BNP and CNP was relieved by EGF-like growth factors, whose expression naturally 
      increases in granulosa cells 18 hr after human chorionic gonadotropin injection. 
      LH and the EGF-like peptide amphiregulin (AREG) decreased BNP and CNP production 
      in granulosa cells and down-regulated NPR2 expression in cumulus cells, which 
      together decreased oocyte cGMP to levels that permit meiotic resumption. The 
      effects of AREG on the gene expression of natriuretic-peptide signaling 
      components and on oocyte maturation were completely blocked by the EGF receptor 
      kinase inhibitor AG1478; the effect of LH, however, was only partially reversed 
      by AG1478. Based on these results, LH regulates natriuretic-peptide signaling, 
      although other pathways also cooperate with the EGF network to induce porcine 
      oocyte maturation.
CI  - (c) 2014 Wiley Periodicals, Inc.
FAU - Zhang, Wenqiang
AU  - Zhang W
AD  - State Key Laboratory of Agro-Biotechnology, College of Biological Sciences, China 
      Agricultural University, Beijing, 100193, People's Republic of China.
FAU - Chen, Qian
AU  - Chen Q
FAU - Yang, Ye
AU  - Yang Y
FAU - Liu, Wei
AU  - Liu W
FAU - Zhang, Meijia
AU  - Zhang M
FAU - Xia, Guoliang
AU  - Xia G
FAU - Wang, Chao
AU  - Wang C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20141027
PL  - United States
TA  - Mol Reprod Dev
JT  - Molecular reproduction and development
JID - 8903333
RN  - 0 (Amphiregulin)
RN  - 0 (DNA Primers)
RN  - 0 (Quinazolines)
RN  - 0 (Tyrphostins)
RN  - 114471-18-0 (Natriuretic Peptide, Brain)
RN  - 127869-51-6 (Natriuretic Peptide, C-Type)
RN  - 170449-18-0 (RTKI cpd)
RN  - 9002-67-9 (Luteinizing Hormone)
RN  - EC 4.6.1.2 (Receptors, Atrial Natriuretic Factor)
RN  - H2D2X058MU (Cyclic GMP)
SB  - IM
MH  - Amphiregulin/*metabolism
MH  - Animals
MH  - Cyclic GMP/biosynthesis
MH  - DNA Primers/genetics
MH  - Female
MH  - Granulosa Cells/metabolism
MH  - In Situ Hybridization
MH  - Luteinizing Hormone/*metabolism
MH  - Meiosis/*physiology
MH  - Natriuretic Peptide, Brain/*metabolism
MH  - Natriuretic Peptide, C-Type/*metabolism
MH  - Oocytes/*physiology
MH  - Quinazolines/pharmacology
MH  - Radioimmunoassay
MH  - Real-Time Polymerase Chain Reaction
MH  - Receptors, Atrial Natriuretic Factor/*metabolism
MH  - Signal Transduction/*physiology
MH  - Swine
MH  - Tyrphostins/pharmacology
EDAT- 2014/10/29 06:00
MHDA- 2015/07/15 06:00
CRDT- 2014/10/29 06:00
PHST- 2014/06/17 00:00 [received]
PHST- 2014/09/07 00:00 [accepted]
PHST- 2014/10/29 06:00 [entrez]
PHST- 2014/10/29 06:00 [pubmed]
PHST- 2015/07/15 06:00 [medline]
AID - 10.1002/mrd.22424 [doi]
PST - ppublish
SO  - Mol Reprod Dev. 2014 Nov;81(11):1030-41. doi: 10.1002/mrd.22424. Epub 2014 Oct 
      27.