PMID- 25349785
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20141028
LR  - 20181113
IS  - 2211-5463 (Print)
IS  - 2211-5463 (Electronic)
IS  - 2211-5463 (Linking)
VI  - 4
DP  - 2014
TI  - Heat shock preconditioning protects against ER stress-induced apoptosis through 
      the regulation of the BH3-only protein BIM.
PG  - 813-21
LID - 10.1016/j.fob.2014.09.004 [doi]
AB  - A mild heat shock (HS) preconditioning and acquisition of thermotolerance 
      protects cells against a variety of cytotoxic agents that otherwise induce 
      apoptosis. Here we tested whether there is a molecular link between HS 
      preconditioning and endoplasmic reticulum (ER) stress-induced apoptosis. ER 
      stress results from a loss of ER lumen homeostasis, culminating in an 
      accumulation of unfolded/misfolded proteins in the ER and activation of unfolded 
      protein response (UPR). Unresolved, ER stress leads to activation of BH3-only 
      proteins, mitochondrial membrane permeabilization, caspase activation and 
      apoptotic cell death. HS preconditioning (1 h at 42  degrees C) induced a rapid increase 
      in HSPA1 (HSP70) levels which remained elevated for at least 48 h post-HS. HS 
      preconditioning significantly reduced BAX, caspase activation and apoptosis in 
      cell cultures treated with the ER stress-inducing agents thapsigargin (TG) and 
      tunicamycin (TM). HS-mediated protection was found to be due to regulation of the 
      BH3-only protein BIM. Further, overexpression of HSPA1 could not mimic the effect 
      of HS on BIM expression, suggesting that other HS factors may play a role in 
      inhibiting ER stress-induced apoptosis by regulating BIM.
FAU - Kennedy, Donna
AU  - Kennedy D
AD  - Apoptosis Research Centre, NUI Galway, Ireland.
FAU - Mnich, Katarzyna
AU  - Mnich K
AD  - Apoptosis Research Centre, NUI Galway, Ireland.
FAU - Samali, Afshin
AU  - Samali A
AD  - Apoptosis Research Centre, NUI Galway, Ireland.
LA  - eng
PT  - Journal Article
DEP - 20140917
PL  - England
TA  - FEBS Open Bio
JT  - FEBS open bio
JID - 101580716
PMC - PMC4208087
OTO - NOTNLM
OT  - Apoptosis
OT  - BCL-2 family
OT  - BIM
OT  - ER, endoplasmic reticulum
OT  - Endoplasmic reticulum stress
OT  - HS, heat shock
OT  - Heat shock
OT  - TG, thapsigargin
OT  - TM, tunicamycin
OT  - UPR, unfolded protein response
OT  - Unfolded protein response
EDAT- 2014/10/29 06:00
MHDA- 2014/10/29 06:01
PMCR- 2014/09/17
CRDT- 2014/10/29 06:00
PHST- 2014/06/10 00:00 [received]
PHST- 2014/08/11 00:00 [revised]
PHST- 2014/09/10 00:00 [accepted]
PHST- 2014/10/29 06:00 [entrez]
PHST- 2014/10/29 06:00 [pubmed]
PHST- 2014/10/29 06:01 [medline]
PHST- 2014/09/17 00:00 [pmc-release]
AID - S2211-5463(14)00086-2 [pii]
AID - 10.1016/j.fob.2014.09.004 [doi]
PST - epublish
SO  - FEBS Open Bio. 2014 Sep 17;4:813-21. doi: 10.1016/j.fob.2014.09.004. eCollection 
      2014.