PMID- 25350560
OWN - NLM
STAT- MEDLINE
DCOM- 20160108
LR  - 20211021
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 9
IP  - 10
DP  - 2014
TI  - PKCdelta and theta possibly mediate FSH-induced mouse oocyte maturation via NOX-ROS-TACE 
      cascade signaling pathway.
PG  - e111423
LID - 10.1371/journal.pone.0111423 [doi]
LID - e111423
AB  - In mammals, gonadotropins stimulate oocyte maturation via the epidermal growth 
      factor (EGF) network, and the protein kinase C (PKC) signaling pathway mediates 
      this process. Tumor necrosis factor-alpha converting enzyme (TACE) is an important 
      protein responding to PKC activation. However, the detailed signaling cascade 
      between PKC and TACE in follicle-stimulating hormone (FSH)-induced oocyte 
      maturation in vitro remains unclear. In this study, we found that rottlerin 
      (mallotoxin, MTX), the inhibitor of PKC delta and theta, blocked FSH-induced maturation 
      of mouse cumulus-oocyte complexes (COCs) in vitro. We further clarified the 
      relationship between two molecules downstream of PKC delta and theta and TACE in COCs: 
      nicotinamide adenine dinucleotide phosphate (NADPH) oxidase (NOX) and its 
      products, reactive oxygen species (ROS). We proved that the respective inhibitors 
      of NOX, ROS and TACE could block FSH-stimulated oocyte maturation 
      dose-dependently, but these inhibitory effects could be reversed partially by 
      amphiregulin (Areg), an EGF family member. Notably, inhibition of PKC delta and theta 
      prevented FSH-induced translocation of two cytosolic components of NOX, p47phox 
      and p67phox, to the plasma membrane in cumulus cells. Moreover, FSH-induced TACE 
      activity in cumulus cells was decreased markedly by inhibition of NOX and ROS. In 
      conclusion, PKC delta and theta possibly mediate FSH-induced meiotic resumption in mouse 
      COCs via NOX-ROS-TACE signaling pathway.
FAU - Chen, Qian
AU  - Chen Q
AD  - State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China 
      Agricultural University, Beijing, People's Republic of China.
FAU - Zhang, Wenqiang
AU  - Zhang W
AD  - State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China 
      Agricultural University, Beijing, People's Republic of China.
FAU - Ran, Hao
AU  - Ran H
AD  - State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China 
      Agricultural University, Beijing, People's Republic of China.
FAU - Feng, Lizhao
AU  - Feng L
AD  - State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China 
      Agricultural University, Beijing, People's Republic of China.
FAU - Yan, Hao
AU  - Yan H
AD  - State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China 
      Agricultural University, Beijing, People's Republic of China.
FAU - Mu, Xinyi
AU  - Mu X
AD  - Department of histology and embryology, Chongqing medical university, Chongqing, 
      People's Republic of China.
FAU - Han, Yingying
AU  - Han Y
AD  - College of biological sciences and technology, Beijing Forestry University, 
      Beijing, People's Republic of China.
FAU - Liu, Wei
AU  - Liu W
AD  - State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China 
      Agricultural University, Beijing, People's Republic of China.
FAU - Xia, Guoliang
AU  - Xia G
AD  - State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China 
      Agricultural University, Beijing, People's Republic of China.
FAU - Wang, Chao
AU  - Wang C
AD  - State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China 
      Agricultural University, Beijing, People's Republic of China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20141028
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Acetophenones)
RN  - 0 (Amphiregulin)
RN  - 0 (Benzopyrans)
RN  - 0 (Isoenzymes)
RN  - 0 (Reactive Oxygen Species)
RN  - 9002-68-0 (Follicle Stimulating Hormone)
RN  - E29LP3ZMUH (rottlerin)
RN  - EC 1.6.- (NADH, NADPH Oxidoreductases)
RN  - EC 1.6.3.- (NADPH Oxidase 1)
RN  - EC 2.7.11.13 (Prkcq protein, mouse)
RN  - EC 2.7.11.13 (Protein Kinase C)
RN  - EC 2.7.11.13 (Protein Kinase C-delta)
RN  - EC 2.7.11.13 (Protein Kinase C-theta)
RN  - EC 3.4.24.- (ADAM Proteins)
RN  - EC 3.4.24.86 (ADAM17 Protein)
RN  - EC 3.4.24.86 (Adam17 protein, mouse)
SB  - IM
MH  - ADAM Proteins/*metabolism
MH  - ADAM17 Protein
MH  - Acetophenones/chemistry
MH  - Amphiregulin/metabolism
MH  - Animals
MH  - Benzopyrans/chemistry
MH  - Body Weight
MH  - Cells, Cultured
MH  - Female
MH  - Follicle Stimulating Hormone/*metabolism
MH  - Isoenzymes/*metabolism
MH  - Meiosis
MH  - Mice
MH  - Microscopy, Fluorescence
MH  - NADH, NADPH Oxidoreductases/metabolism
MH  - NADPH Oxidase 1
MH  - Oocytes/cytology/*drug effects/physiology
MH  - Phagocytosis
MH  - Protein Kinase C/*metabolism
MH  - Protein Kinase C-delta/*metabolism
MH  - Protein Kinase C-theta
MH  - Reactive Oxygen Species/metabolism
MH  - *Signal Transduction
PMC - PMC4211700
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2014/10/29 06:00
MHDA- 2016/01/09 06:00
PMCR- 2014/10/28
CRDT- 2014/10/29 06:00
PHST- 2014/07/07 00:00 [received]
PHST- 2014/09/25 00:00 [accepted]
PHST- 2014/10/29 06:00 [entrez]
PHST- 2014/10/29 06:00 [pubmed]
PHST- 2016/01/09 06:00 [medline]
PHST- 2014/10/28 00:00 [pmc-release]
AID - PONE-D-14-30250 [pii]
AID - 10.1371/journal.pone.0111423 [doi]
PST - epublish
SO  - PLoS One. 2014 Oct 28;9(10):e111423. doi: 10.1371/journal.pone.0111423. 
      eCollection 2014.