PMID- 25351155
OWN - NLM
STAT- MEDLINE
DCOM- 20150515
LR  - 20191027
IS  - 1678-2674 (Electronic)
IS  - 0102-8650 (Linking)
VI  - 29 Suppl 3
DP  - 2014
TI  - Human beta defensin-4 and keratinocyte growth factor gene expression in cultured 
      keratinocyte and fibroblasts of burned patients.
PG  - 39-43
LID - S0102-86502014001500039 [pii]
AB  - PURPOSE: To evaluate KGF and human beta defensin-4 (HBD-4) levels produced by 
      dermic fibroblasts and keratinocytes cultivated from burned patients' skin 
      samples. METHODS: Keratinocytes and fibroblasts of 10 patients (four major burns, 
      four minor burns and two controls) were primarily cultivated according to 
      standard methods. HBD-4 and KGF genes were analyzed by quantitative PCR. RESULTS: 
      In fibroblasts, KGF gene expression was 220+/-80 and 33.33+/-6.67 (M+/-SD; N=4), 
      respectively for major and minor burn groups. In keratinocytes, KGF gene 
      expression was 11.2+/-1.9 and 3.45+/-0.37 (M+/-SD; N=4), respectively for major and 
      minor burn groups. In fibroblasts, HBD-4 gene expression was 15.0+/-4.0 and 
      11.5+/-0.5 (M+/-SD; N=4), respectively for major and minor burn. In keratinocyte, 
      HBD-4 gene expression was 0.0+/-0.0 and 13.4+/-4.8 (M+/-SD; N=4), respectively for 
      major and minor burn. CONCLUSIONS: KGF expression was increased in burn patient 
      fibroblasts compared to control group. In keratinocytes culture, KGF suppression 
      is inversely proportional to burn extension; it is active and increased in major 
      burn but decreased in minor burn. HBD-4 expression was increased in fibroblasts 
      and decreased in keratinocytes from all burned patients.
FAU - Noronha, Silvana Aparecida Alves Correa de
AU  - Noronha SA
AD  - Department of Surgery, EPM, UNIFESP, Sao Paulo, SP, Brazil.
FAU - Noronha, Samuel Marcos Ribeiro de
AU  - Noronha SM
AD  - Department of Surgery, EPM, UNIFESP, Sao Paulo, SP, Brazil.
FAU - Lanziani, Larissa Elias
AU  - Lanziani LE
AD  - EPM, UNIFESP, Sao Paulo, SP, Brazil.
FAU - Ipolito, Michele Zampieri
AU  - Ipolito MZ
AD  - EPM, UNIFESP, Sao Paulo, SP, Brazil.
FAU - Ferreira, Lydia Masako
AU  - Ferreira LM
AD  - Department of Surgery, EPM, UNIFESP, Sao Paulo, SP, Brazil.
FAU - Gragnani, Alfredo
AU  - Gragnani A
AD  - Department of Surgery, Paulista School of Medicine, Federal University of Sao 
      Paulo, Brazil.
LA  - eng
PT  - Controlled Clinical Trial
PT  - Journal Article
PT  - Observational Study
PT  - Research Support, Non-U.S. Gov't
PL  - Brazil
TA  - Acta Cir Bras
JT  - Acta cirurgica brasileira
JID - 9103983
RN  - 0 (DEFB4A protein, human)
RN  - 0 (beta-Defensins)
RN  - 126469-10-1 (Fibroblast Growth Factor 7)
RN  - 63231-63-0 (RNA)
SB  - IM
MH  - Burns/*genetics
MH  - Cells, Cultured
MH  - Female
MH  - Fibroblast Growth Factor 7/*analysis/genetics
MH  - Fibroblasts/*metabolism
MH  - Gene Expression
MH  - Humans
MH  - Keratinocytes/*metabolism
MH  - Male
MH  - Polymerase Chain Reaction
MH  - RNA/isolation & purification
MH  - Skin/cytology/injuries
MH  - Young Adult
MH  - beta-Defensins/*genetics/metabolism
EDAT- 2014/10/30 06:00
MHDA- 2015/05/16 06:00
CRDT- 2014/10/30 06:00
PHST- 2014/10/30 06:00 [entrez]
PHST- 2014/10/30 06:00 [pubmed]
PHST- 2015/05/16 06:00 [medline]
AID - S0102-86502014001500039 [pii]
AID - 10.1590/s0102-86502014001700008 [doi]
PST - ppublish
SO  - Acta Cir Bras. 2014;29 Suppl 3:39-43. doi: 10.1590/s0102-86502014001700008.