PMID- 25351221
OWN - NLM
STAT- MEDLINE
DCOM- 20150527
LR  - 20181202
IS  - 1002-0098 (Print)
IS  - 1002-0098 (Linking)
VI  - 49
IP  - 8
DP  - 2014 Aug
TI  - [Studies on the correlation between the expression of Toll-like receptor 4 and 
      the synovitis of the temporomandibular joint in rats].
PG  - 481-5
AB  - OBJECTIVE: To investigate the expression of the Toll-like receptor-4 (TLR-4) in 
      temporo-mandibular joint synovitis in rats, and to discuss the correlation 
      between the expression of TLR-4 and the synovitis. METHODS: Sixty male wistar 
      rats were randomly divided into five groups, 12 each. Group A was the control 
      group in which the rats were given normal diet.In Group B, the rats' bilateral 
      masseter muscles were cut off (masseter resection group). In Group C, An cast 
      metal crown were bonded on the mandibular right first molar of each rat (occlusal 
      interference group). In Group D, occlusal pad were bonded on maxillary molars of 
      each rat (occlusal dimension increase group). In Group E, rats' bilateral 
      masseter muscles were re-sected and occlusal pads were bonded on their maxillary 
      molars (masseter resection and occlusal dimension increase group). Pathological 
      changes of synovium were observed using hematoxylin and eosin (HE) stains and 
      pathology scores were evaluated. The expression of TLR- 4 were determined by 
      immunohistochemical stains, and the expression of TLR-4 mRNA were determined by 
      real-time PCR. The correlation between the expression of TLR-4, TLR-4 mRNA and 
      the pathological score were analyzed using Spearman analysis. RESULTS: The 
      pathological scores of Group A-E were 0.5 +/- 0.5, 2.5 +/- 1.0, 2.7 +/- 1.0, 3.0 +/- 0.9, 
      5.3 +/- 1.2 respectively. The expression of TLR-4 were (3.2 +/- 1.5)%, (16.+/- 2.6)%, 
      (15.8 +/- 2.1)%, (17.5 +/- 2.4)%, (38.2 +/- 4.4) %. The expression of TLR-4 mRNA were 
      1.07 +/- 0.09, 2.12 +/- 0.33, 2.07 +/- 0.29, 2.17 +/- 0.34, 4.53 +/- 0.46. Compared with 
      group A, groups B- E showed significant higher pathology score (P < 0.05) and 
      increased expression of both TLR-4 (P < 0.05) and TLR-4 mRNA (P < 0.05). An 
      significant positive correlation was found between the expression of TLR- 4 and 
      the pathology score (r = 0.785, P < 0.05), and between the expression of TLR- 4 
      mRNA and the pathology score (r = 0.720, P < 0.05). CONCLUSIONS: TLR-4 may be 
      closely associated with the development of the synovitis of TMJ of rats.
FAU - Kong, Jingjing
AU  - Kong J
AD  - Department of Prosthodontics, School of Stomatology, Shandong University; 
      Shandong Provincial Key Laboratory of Oral Biomedicine, Jinan 250012, China.
FAU - Wu, Qingting
AU  - Wu Q
AD  - Department of Prosthodontics, School of Stomatology, Shandong University; 
      Shandong Provincial Key Laboratory of Oral Biomedicine, Jinan 250012, China.
FAU - Wang, Xiaohui
AU  - Wang X
AD  - Department of Prosthodontics, School of Stomatology, Shandong University; 
      Shandong Provincial Key Laboratory of Oral Biomedicine, Jinan 250012, China.
FAU - Yang, Yingying
AU  - Yang Y
AD  - Department of Prosthodontics, School of Stomatology, Shandong University; 
      Shandong Provincial Key Laboratory of Oral Biomedicine, Jinan 250012, China.
FAU - Lin, Xuefen
AU  - Lin X
AD  - Department of Prosthodontics, School of Stomatology, Shandong University; 
      Shandong Provincial Key Laboratory of Oral Biomedicine, Jinan 250012, China.
FAU - Ji, Ping
AU  - Ji P
AD  - Department of Prosthodontics, School of Stomatology, Shandong University; 
      Shandong Provincial Key Laboratory of Oral Biomedicine, Jinan 250012, China. 
      Email: jiping@mail.sdu.edu.cn.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhonghua Kou Qiang Yi Xue Za Zhi
JT  - Zhonghua kou qiang yi xue za zhi = Zhonghua kouqiang yixue zazhi = Chinese 
      journal of stomatology
JID - 8711066
RN  - 0 (RNA, Messenger)
RN  - 0 (Tlr4 protein, rat)
RN  - 0 (Toll-Like Receptor 4)
SB  - IM
MH  - Animals
MH  - Male
MH  - Masseter Muscle
MH  - RNA, Messenger
MH  - Rats
MH  - Rats, Wistar
MH  - Synovial Membrane
MH  - Synovitis/*metabolism
MH  - Temporomandibular Joint/*metabolism
MH  - Toll-Like Receptor 4/*biosynthesis
EDAT- 2014/10/30 06:00
MHDA- 2015/05/28 06:00
CRDT- 2014/10/30 06:00
PHST- 2014/10/30 06:00 [entrez]
PHST- 2014/10/30 06:00 [pubmed]
PHST- 2015/05/28 06:00 [medline]
PST - ppublish
SO  - Zhonghua Kou Qiang Yi Xue Za Zhi. 2014 Aug;49(8):481-5.