PMID- 25352084
OWN - NLM
STAT- MEDLINE
DCOM- 20150625
LR  - 20211021
IS  - 1791-3004 (Electronic)
IS  - 1791-2997 (Print)
IS  - 1791-2997 (Linking)
VI  - 11
IP  - 1
DP  - 2015 Jan
TI  - Inhibitory effects of C-type natriuretic peptide on the differentiation of 
      cardiac fibroblasts, and secretion of monocyte chemoattractant protein-1 and 
      plasminogen activator inhibitor-1.
PG  - 159-65
LID - 10.3892/mmr.2014.2763 [doi]
AB  - The present study aimed to investigate the effect of C-type natriuretic peptide 
      (CNP) on the function of cardiac fibroblasts (CFs). Western blotting was used to 
      investigate the expression of myofibroblast marker proteins: alpha-smooth muscle 
      actin (alpha-SMA), extra domain-A fibronectin, collagen I and collagen III, and the 
      activity of extracellular signal-regulated kinase 1/2 (ERK1/2). 
      Immunofluorescence was used to examine the morphological changes; a transwell 
      assay was used to analyze migration, and reverse transcription-quantitative 
      polymerase chain reaction and ELISA were employed to determine the mRNA 
      expression and protein secretion of monocyte chemoattractant protein-1 (MCP-1) 
      and plasminogen activator inhibitor-1 (PAI-1). The results demonstrated that CNP 
      significantly reduced the protein expression of alpha-SMA, fibronectin, collagen I 
      and collagen III, and suppressed the migratory ability of CFs. Additionally, the 
      mRNA and protein expression of MCP-1 and PAI-1 was inhibited under the CNP 
      treatment; and this effect was mediated by the inhibition of the ERK1/2 activity. 
      In conclusion, CNP inhibited cardiac fibroblast differentiation and migration, 
      and reduced the secretion of MCP-1 and PAI-1, which demonstrates novel mechanisms 
      to explain the antifibrotic effect of CNP.
FAU - Li, Zhi-Qiang
AU  - Li ZQ
AD  - Department of Pediatric Cardiac Surgery Center, Beijing Anzhen Hospital, Capital 
      Medical University, Beijing 100029, P.R. China.
FAU - Liu, Ying-Long
AU  - Liu YL
AD  - Department of Pediatric Cardiac Surgery Center, Beijing Anzhen Hospital, Capital 
      Medical University, Beijing 100029, P.R. China.
FAU - Li, Gang
AU  - Li G
AD  - Department of Pediatric Cardiac Surgery Center, Beijing Anzhen Hospital, Capital 
      Medical University, Beijing 100029, P.R. China.
FAU - Li, Bin
AU  - Li B
AD  - Department of Pediatric Cardiac Surgery Center, Beijing Anzhen Hospital, Capital 
      Medical University, Beijing 100029, P.R. China.
FAU - Liu, Yang
AU  - Liu Y
AD  - Department of Pediatric Cardiac Surgery Center, Beijing Anzhen Hospital, Capital 
      Medical University, Beijing 100029, P.R. China.
FAU - Li, Xiao-Feng
AU  - Li XF
AD  - Department of Pediatric Cardiac Surgery Center, Beijing Anzhen Hospital, Capital 
      Medical University, Beijing 100029, P.R. China.
FAU - Liu, Ai-Jun
AU  - Liu AJ
AD  - Department of Pediatric Cardiac Surgery Center, Beijing Anzhen Hospital, Capital 
      Medical University, Beijing 100029, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20141023
PL  - Greece
TA  - Mol Med Rep
JT  - Molecular medicine reports
JID - 101475259
RN  - 0 (Chemokine CCL2)
RN  - 0 (Plasminogen Activator Inhibitor 1)
RN  - 127869-51-6 (Natriuretic Peptide, C-Type)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinase 1)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinase 3)
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - Cell Differentiation/*drug effects
MH  - Cell Movement/drug effects
MH  - Chemokine CCL2/genetics/*metabolism
MH  - Enzyme Activation/drug effects
MH  - Gene Expression
MH  - Mitogen-Activated Protein Kinase 1/metabolism
MH  - Mitogen-Activated Protein Kinase 3/metabolism
MH  - Myoblasts, Cardiac/*cytology/*metabolism
MH  - Myofibroblasts/*cytology/*metabolism
MH  - Natriuretic Peptide, C-Type/*pharmacology
MH  - Plasminogen Activator Inhibitor 1/genetics/*metabolism
MH  - Primary Cell Culture
MH  - Rats
PMC - PMC4237089
EDAT- 2014/10/30 06:00
MHDA- 2015/06/26 06:00
PMCR- 2014/10/23
CRDT- 2014/10/30 06:00
PHST- 2013/12/06 00:00 [received]
PHST- 2014/05/28 00:00 [accepted]
PHST- 2014/10/30 06:00 [entrez]
PHST- 2014/10/30 06:00 [pubmed]
PHST- 2015/06/26 06:00 [medline]
PHST- 2014/10/23 00:00 [pmc-release]
AID - mmr-11-01-0159 [pii]
AID - 10.3892/mmr.2014.2763 [doi]
PST - ppublish
SO  - Mol Med Rep. 2015 Jan;11(1):159-65. doi: 10.3892/mmr.2014.2763. Epub 2014 Oct 23.