PMID- 25352778 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20141029 LR - 20211021 IS - 1662-5102 (Print) IS - 1662-5102 (Electronic) IS - 1662-5102 (Linking) VI - 8 DP - 2014 TI - Use of induced pluripotent stem cell derived neurons engineered to express BDNF for modulation of stressor related pathology. PG - 316 LID - 10.3389/fncel.2014.00316 [doi] LID - 316 AB - Combined cell and gene-based therapeutic strategies offer potential in the treatment of neurodegenerative and psychiatric conditions that have been associated with structural brain disturbances. In the present investigation, we used a novel virus-free re-programming method to generate induced pluripotent stem cells (iPSCs), and then subsequently transformed these cells into neural cells which over-expressed brain derived neurotrophic factor (BDNF). Importantly, the infusion of iPSC derived neural cells (as a cell replacement and gene delivery tool) and BDNF (as a protective factor) influenced neuronal outcomes. Specifically, intracerebroventricular transplantation of iPSC-derived neural progenitors that over-expressed BDNF reversed the impact of immune (lipopolysaccharide) and chronic stressor challenges upon subventricular zone adult neurogenesis, and the iPSC-derived neural progenitor cells alone blunted the stressor-induced corticosterone response. Moreover, our findings indicate that mature dopamine producing neurons can be generated using iPSC procedures and appear to be viable when infused in vivo. Taken together, these data could have important implications for using gene-plus-cell replacement methods to modulate stressor related pathology. FAU - Liu, Gele AU - Liu G AD - Hayley Lab, Department of Neuroscience, Carleton University Ottawa, ON, Canada. FAU - Rustom, Nazneen AU - Rustom N AD - Hayley Lab, Department of Neuroscience, Carleton University Ottawa, ON, Canada. FAU - Litteljohn, Darcy AU - Litteljohn D AD - Hayley Lab, Department of Neuroscience, Carleton University Ottawa, ON, Canada. FAU - Bobyn, Jessica AU - Bobyn J AD - Hayley Lab, Department of Neuroscience, Carleton University Ottawa, ON, Canada. FAU - Rudyk, Chris AU - Rudyk C AD - Hayley Lab, Department of Neuroscience, Carleton University Ottawa, ON, Canada. FAU - Anisman, Hymie AU - Anisman H AD - Hayley Lab, Department of Neuroscience, Carleton University Ottawa, ON, Canada. FAU - Hayley, Shawn AU - Hayley S AD - Hayley Lab, Department of Neuroscience, Carleton University Ottawa, ON, Canada. LA - eng PT - Journal Article DEP - 20141014 PL - Switzerland TA - Front Cell Neurosci JT - Frontiers in cellular neuroscience JID - 101477935 PMC - PMC4196567 OTO - NOTNLM OT - BDNF OT - depression OT - neurogenesis OT - stem cell OT - stress EDAT- 2014/10/30 06:00 MHDA- 2014/10/30 06:01 PMCR- 2014/01/01 CRDT- 2014/10/30 06:00 PHST- 2014/05/30 00:00 [received] PHST- 2014/09/20 00:00 [accepted] PHST- 2014/10/30 06:00 [entrez] PHST- 2014/10/30 06:00 [pubmed] PHST- 2014/10/30 06:01 [medline] PHST- 2014/01/01 00:00 [pmc-release] AID - 10.3389/fncel.2014.00316 [doi] PST - epublish SO - Front Cell Neurosci. 2014 Oct 14;8:316. doi: 10.3389/fncel.2014.00316. eCollection 2014.