PMID- 25352856
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20141029
LR  - 20211021
IS  - 1664-462X (Print)
IS  - 1664-462X (Electronic)
IS  - 1664-462X (Linking)
VI  - 5
DP  - 2014
TI  - Karyotype variability in tropical maize sister inbred lines and hybrids compared 
      with KYS standard line.
PG  - 544
LID - 10.3389/fpls.2014.00544 [doi]
LID - 544
AB  - Maize karyotype variability has been extensively investigated. The identification 
      of maize somatic and pachytene chromosomes has improved with the development of 
      fluorescence in situ hybridization (FISH) using tandemly repeated DNA sequences 
      as probes. We identified the somatic chromosomes of sister inbred lines that were 
      derived from a tropical flint maize population (Jac Duro [JD]), and hybrids 
      between them, using FISH probes for the 180-bp knob repeat, centromeric satellite 
      (CentC), centromeric satellite 4 (Cent4), subtelomeric clone 4-12-1, 5S ribosomal 
      DNA and nucleolus organizing region DNA sequences. The observations were 
      integrated with data based on C-banded mitotic metaphases and conventional 
      analysis of pachytene chromosomes. Heterochromatic knobs visible at pachynema 
      were coincident with C-bands and 180-bp FISH signals on somatic chromosomes, and 
      most of them were large. Variation in the presence of some knobs was observed 
      among lines. Small 180-bp knob signals were invariant on the short arms of 
      chromosomes 1, 6, and 9. The subtelomeric 4-12-1 signal was also invariant and 
      useful for identifying some chromosomes. The centromere location of chromosomes 2 
      and 4 differed from previous reports on standard maize lines. Somatic chromosomes 
      of a JD line and the commonly used KYS line were compared by FISH in a hybrid of 
      these lines. The pairing behavior of chromosomes 2 and 4 at pachytene stage in 
      this hybrid was investigated using FISH with chromosome-specific probes. The 
      homologues were fully synapsed, including the 5S rDNA and CentC sites on 
      chromosome 2, and Cent4 and subtelomeric 4-12-1 sites on chromosome 4. This 
      suggests that homologous chromosomes could pair through differential degrees of 
      chromatin packaging in homologous arms differing in size. The results contribute 
      to current knowledge of maize global diversity and also raise questions 
      concerning the meiotic pairing of homologous chromosomes possibly differing in 
      their amounts of repetitive DNA.
FAU - Mondin, Mateus
AU  - Mondin M
AD  - Department of Genetics, Luiz de Queiroz College of Agriculture, University of Sao 
      Paulo, Piracicaba, Brazil.
FAU - Santos-Serejo, Janay A
AU  - Santos-Serejo JA
AD  - Department of Genetics, Luiz de Queiroz College of Agriculture, University of Sao 
      Paulo, Piracicaba, Brazil ; Embrapa Cassava and Fruits, Brazilian Agricultural 
      Research Corporation Cruz das Almas, Brazil.
FAU - Bertao, Monica R
AU  - Bertao MR
AD  - Department of Genetics, Luiz de Queiroz College of Agriculture, University of Sao 
      Paulo, Piracicaba, Brazil ; Department of Biological Sciences, Faculty of 
      Sciences and Letters, Sao Paulo State University Assis, Brazil.
FAU - Laborda, Prianda
AU  - Laborda P
AD  - Center for Molecular Biology and Genetic Engineering, State University of 
      Campinas Campinas, Brazil.
FAU - Pizzaia, Daniel
AU  - Pizzaia D
AD  - Department of Genetics, Luiz de Queiroz College of Agriculture, University of Sao 
      Paulo, Piracicaba, Brazil ; Herminio Ometto University Center, Herminio Ometto 
      Foundation Araras, Brazil.
FAU - Aguiar-Perecin, Margarida L R
AU  - Aguiar-Perecin ML
AD  - Department of Genetics, Luiz de Queiroz College of Agriculture, University of Sao 
      Paulo, Piracicaba, Brazil.
LA  - eng
PT  - Journal Article
DEP - 20141013
PL  - Switzerland
TA  - Front Plant Sci
JT  - Frontiers in plant science
JID - 101568200
PMC - PMC4195276
OTO - NOTNLM
OT  - centromere
OT  - chromosomes
OT  - heterochromatic knobs
OT  - maize
OT  - satellite DNA
EDAT- 2014/10/30 06:00
MHDA- 2014/10/30 06:01
PMCR- 2014/01/01
CRDT- 2014/10/30 06:00
PHST- 2014/07/23 00:00 [received]
PHST- 2014/09/24 00:00 [accepted]
PHST- 2014/10/30 06:00 [entrez]
PHST- 2014/10/30 06:00 [pubmed]
PHST- 2014/10/30 06:01 [medline]
PHST- 2014/01/01 00:00 [pmc-release]
AID - 10.3389/fpls.2014.00544 [doi]
PST - epublish
SO  - Front Plant Sci. 2014 Oct 13;5:544. doi: 10.3389/fpls.2014.00544. eCollection 
      2014.