PMID- 25354905
OWN - NLM
STAT- MEDLINE
DCOM- 20151109
LR  - 20220408
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 4
DP  - 2014 Oct 30
TI  - Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass 
      spectrometry applied to virus identification.
PG  - 6803
LID - 10.1038/srep06803 [doi]
LID - 6803
AB  - Virus detection and/or identification traditionally rely on methods based on cell 
      culture, electron microscopy and antigen or nucleic acid detection. These 
      techniques are good, but often expensive and/or time-consuming; furthermore, they 
      not always lead to virus identification at the species and/or type level. In this 
      study, Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass 
      Spectrometry (MALDI-TOF MS) was tested as an innovative tool to identify human 
      polioviruses and to identify specific viral protein biomarkers in infected cells. 
      The results revealed MALDI-TOF MS to be an effective and inexpensive tool for the 
      identification of the three poliovirus serotypes. The method was firstly applied 
      to Sabin reference strains, and then to isolates from different clinical samples, 
      highlighting its value as a time-saving, sensitive and specific technique when 
      compared to the gold standard neutralization assay and casting new light on its 
      possible application to virus detection and/or identification.
FAU - Calderaro, Adriana
AU  - Calderaro A
AD  - Department of Clinical and Experimental Medicine, Unit of Microbiology and 
      Virology, University of Parma, Italy.
FAU - Arcangeletti, Maria-Cristina
AU  - Arcangeletti MC
AD  - Department of Clinical and Experimental Medicine, Unit of Microbiology and 
      Virology, University of Parma, Italy.
FAU - Rodighiero, Isabella
AU  - Rodighiero I
AD  - Department of Clinical and Experimental Medicine, Unit of Microbiology and 
      Virology, University of Parma, Italy.
FAU - Buttrini, Mirko
AU  - Buttrini M
AD  - Department of Clinical and Experimental Medicine, Unit of Microbiology and 
      Virology, University of Parma, Italy.
FAU - Gorrini, Chiara
AU  - Gorrini C
AD  - Department of Clinical and Experimental Medicine, Unit of Microbiology and 
      Virology, University of Parma, Italy.
FAU - Motta, Federica
AU  - Motta F
AD  - Department of Clinical and Experimental Medicine, Unit of Microbiology and 
      Virology, University of Parma, Italy.
FAU - Germini, Diego
AU  - Germini D
AD  - Department of Clinical and Experimental Medicine, Unit of Microbiology and 
      Virology, University of Parma, Italy.
FAU - Medici, Maria-Cristina
AU  - Medici MC
AD  - Department of Clinical and Experimental Medicine, Unit of Microbiology and 
      Virology, University of Parma, Italy.
FAU - Chezzi, Carlo
AU  - Chezzi C
AD  - Department of Clinical and Experimental Medicine, Unit of Microbiology and 
      Virology, University of Parma, Italy.
FAU - De Conto, Flora
AU  - De Conto F
AD  - Department of Clinical and Experimental Medicine, Unit of Microbiology and 
      Virology, University of Parma, Italy.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20141030
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Viral Proteins)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Enterovirus/classification
MH  - Humans
MH  - Poliovirus/classification/isolation & purification
MH  - Reproducibility of Results
MH  - Serogroup
MH  - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/*methods
MH  - Viral Proteins/chemistry
MH  - Viruses/*classification/isolation & purification
PMC - PMC4213803
EDAT- 2014/10/31 06:00
MHDA- 2015/11/10 06:00
PMCR- 2014/10/30
CRDT- 2014/10/31 06:00
PHST- 2014/04/09 00:00 [received]
PHST- 2014/09/15 00:00 [accepted]
PHST- 2014/10/31 06:00 [entrez]
PHST- 2014/10/31 06:00 [pubmed]
PHST- 2015/11/10 06:00 [medline]
PHST- 2014/10/30 00:00 [pmc-release]
AID - srep06803 [pii]
AID - 10.1038/srep06803 [doi]
PST - epublish
SO  - Sci Rep. 2014 Oct 30;4:6803. doi: 10.1038/srep06803.