PMID- 25355253
OWN - NLM
STAT- MEDLINE
DCOM- 20150908
LR  - 20181202
IS  - 1672-7347 (Print)
IS  - 1672-7347 (Linking)
VI  - 39
IP  - 10
DP  - 2014 Oct
TI  - [Effect of Feridex-GFP double-labeled BMSC transplant on the damaged liver under 
      the condition of constant magnetic field].
PG  - 1008-15
LID - 10.11817/j.issn.1672-7347.2014.10.004 [doi]
AB  - OBJECTIVE: To evaluate the effect of the bone marrow mesenchymal stem cells 
      (BMSCs) transplant through peripheral vein, portal vein and hepatic artery into 
      liver under the condition of constant magnetic field and to analyze the 
      therapeutic effect on liver function recovery. METHODS: BMSCs were isolated, 
      purified and induced to differentiate into liver-like cells, which were double 
      labeled by Feridex-GFP. The double-labeled BMSCs were transplanted into liver 
      through different ways including peripheral vein, portal vein and hepatic artery 
      with or without constant magnetic field in vitro. The rats were sacrificed at the 
      1st, 2nd, 3rd and 4th week after the transplant. ALB, ALT, AST were tested. The 
      liver tissue biopsy was collected. GFP-positive cells in liver were observed by 
      fluorescence microscopy. RESULTS: Double-labeled BMSCs could be transplanted into 
      liver through all ways. GFP expression was found in liver in all groups at the 
      4th week and the liver functions were improved. Based on the long term efficacy, 
      the liver functions recovered more rapidly in the portal vein + constant magnetic 
      field group and the hepatic artery + constant magnetic field group. CONCLUSION: 
      BMSCs transplantation can reduce acute liver damage. The first choice for BMSCs 
      transplantation was via portal vein or hepatic artery under the condition of 
      constant magnetic field. The second choice was via peripheral vein alone or under 
      the condition of constant magnetic field.
FAU - Liu, Shaojun
AU  - Liu S
AD  - Department of Gastroenterology, Third Xiangya Hospital, Central South University, 
      Changsha 410013, China.
FAU - Wang, Xiaoyan
AU  - Wang X
FAU - Xiao, Zhiming
AU  - Xiao Z
FAU - Tang, Anliu
AU  - Tang A
FAU - Tang, Wuliang
AU  - Tang W
FAU - Shen, Shourong
AU  - Shen S
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhong Nan Da Xue Xue Bao Yi Xue Ban
JT  - Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. 
      Medical sciences
JID - 101230586
RN  - 0 (Dextrans)
RN  - 0 (Magnetite Nanoparticles)
RN  - 147336-22-9 (Green Fluorescent Proteins)
RN  - G6N3J05W84 (ferumoxides)
SB  - IM
MH  - Animals
MH  - *Dextrans
MH  - Green Fluorescent Proteins
MH  - Liver Diseases/*therapy
MH  - *Magnetic Fields
MH  - *Magnetite Nanoparticles
MH  - *Mesenchymal Stem Cell Transplantation
MH  - Rats
EDAT- 2014/10/31 06:00
MHDA- 2015/09/09 06:00
CRDT- 2014/10/31 06:00
PHST- 2014/10/31 06:00 [entrez]
PHST- 2014/10/31 06:00 [pubmed]
PHST- 2015/09/09 06:00 [medline]
AID - 10.11817/j.issn.1672-7347.2014.10.004 [doi]
PST - ppublish
SO  - Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2014 Oct;39(10):1008-15. doi: 
      10.11817/j.issn.1672-7347.2014.10.004.