PMID- 25355794
OWN - NLM
STAT- MEDLINE
DCOM- 20150827
LR  - 20211021
IS  - 1556-679X (Electronic)
IS  - 1556-6811 (Print)
IS  - 1556-679X (Linking)
VI  - 22
IP  - 1
DP  - 2015 Jan
TI  - A eukaryotic expression plasmid carrying chicken interleukin-18 enhances the 
      response to newcastle disease virus vaccine.
PG  - 56-64
LID - 10.1128/CVI.00636-14 [doi]
AB  - Interleukin-18 (IL-18) is an important cytokine involved in innate and acquired 
      immunity. In this study, we cloned the full-length chicken IL-18 (ChIL-18) gene 
      from specific-pathogen-free (SPF) chicken embryo spleen cells and provided 
      evidence that the ChIL-18 gene in a recombinant plasmid was successfully 
      expressed in chicken DT40 cells. ChIL-18 significantly enhanced gamma interferon 
      (IFN-gamma) mRNA expression in chicken splenocytes, which increased IFN-gamma-induced 
      nitric oxide (NO) synthesis by macrophages. The potential genetic adjuvant 
      activity of the ChIL-18 plasmid was examined in chickens by coinjecting ChIL-18 
      plasmid and inactivated Newcastle disease virus (NDV) vaccine. ChIL-18 markedly 
      elevated serum hemagglutination inhibition (HI) titers and 
      anti-hemagglutinin-neuraminidase (anti-HN)-specific antibody levels, induced the 
      secretion of both Th1- (IFN-gamma) and Th2- (interleukin-4) type cytokines, promoted 
      the proliferation of T and B lymphocytes, and increased the populations of CD3(+) 
      T cells and their subsets, CD3(+) CD4(+) and CD3(+) CD8(+) T cells. Furthermore, 
      a virus challenge revealed that ChIL-18 contributed to protection against 
      Newcastle disease virus challenge. Taken together, our data indicate that the 
      coadministration of ChIL-18 plasmid and NDV vaccine induces a strong immune 
      response at both the humoral and cellular levels and that ChIL-18 is a novel 
      immunoadjuvant suitable for NDV vaccination.
CI  - Copyright (c) 2015, American Society for Microbiology. All Rights Reserved.
FAU - Wang, Chen
AU  - Wang C
AD  - College of Animal Science and Technology, Henan University of Science and 
      Technology, Luoyang, China wangchen2001@126.com xiangchaoc1966@126.com.
FAU - Li, Xiaokang
AU  - Li X
AD  - College of Animal Science and Technology, Henan University of Science and 
      Technology, Luoyang, China.
FAU - Zhang, Chunjie
AU  - Zhang C
AD  - College of Animal Science and Technology, Henan University of Science and 
      Technology, Luoyang, China.
FAU - Wu, Tingcai
AU  - Wu T
AD  - College of Animal Science and Technology, Henan University of Science and 
      Technology, Luoyang, China.
FAU - Li, Yinju
AU  - Li Y
AD  - College of Animal Science and Technology, Henan University of Science and 
      Technology, Luoyang, China.
FAU - Cheng, Xiangchao
AU  - Cheng X
AD  - College of Animal Science and Technology, Henan University of Science and 
      Technology, Luoyang, China wangchen2001@126.com xiangchaoc1966@126.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20141029
PL  - United States
TA  - Clin Vaccine Immunol
JT  - Clinical and vaccine immunology : CVI
JID - 101252125
RN  - 0 (Adjuvants, Immunologic)
RN  - 0 (Antibodies, Viral)
RN  - 0 (Antigens, Viral)
RN  - 0 (Interleukin-18)
RN  - 0 (Vaccines, Inactivated)
RN  - 0 (Viral Vaccines)
SB  - IM
MH  - Adjuvants, Immunologic/genetics/*metabolism
MH  - Animals
MH  - Antibodies, Viral/blood
MH  - Antigens, Viral/immunology
MH  - B-Lymphocytes/immunology
MH  - Cell Proliferation
MH  - Chickens
MH  - *Gene Expression
MH  - Hemagglutination Inhibition Tests
MH  - Interleukin-18/genetics/*metabolism
MH  - Newcastle Disease/immunology/*prevention & control
MH  - Newcastle disease virus/immunology
MH  - T-Lymphocyte Subsets/immunology
MH  - Vaccines, Inactivated/administration & dosage/immunology
MH  - Viral Vaccines/administration & dosage/*immunology
PMC - PMC4278935
EDAT- 2014/10/31 06:00
MHDA- 2015/08/28 06:00
PMCR- 2015/07/01
CRDT- 2014/10/31 06:00
PHST- 2014/10/31 06:00 [entrez]
PHST- 2014/10/31 06:00 [pubmed]
PHST- 2015/08/28 06:00 [medline]
PHST- 2015/07/01 00:00 [pmc-release]
AID - CVI.00636-14 [pii]
AID - 00636-14 [pii]
AID - 10.1128/CVI.00636-14 [doi]
PST - ppublish
SO  - Clin Vaccine Immunol. 2015 Jan;22(1):56-64. doi: 10.1128/CVI.00636-14. Epub 2014 
      Oct 29.