PMID- 25356348
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20141030
LR  - 20211021
IS  - 2052-2975 (Print)
IS  - 2052-2975 (Electronic)
IS  - 2052-2975 (Linking)
VI  - 2
IP  - 3
DP  - 2014 May
TI  - Rapid identification of Stenotrophomonas maltophilia by peptide nucleic acid 
      fluorescence in situ hybridization.
PG  - 79-81
LID - 10.1002/nmi2.38 [doi]
AB  - The objective of this study was to develop a novel peptide nucleic acid (PNA) 
      probe for Stenotrophomonas maltophilia identification by fluorescence in situ 
      hybridization (FISH). The probe was evaluated using 33 human and veterinary 
      clinical S. maltophilia isolates and 45 reference strains representing common 
      bacterial species in the respiratory tract. The probe displayed 100% sensitivity 
      and 100% specificity on pure cultures and allowed detection in sputum from cystic 
      fibrosis patients. The detection limit was 10(4) CFU/mL in spiked tracheal 
      aspirate and bronchoalveolar lavage from healthy horses. Altogether the study 
      shows that this species-specific PNA FISH probe facilitates rapid detection of S. 
      maltophilia in biological specimens.
FAU - Hansen, N
AU  - Hansen N
AD  - Department of Veterinary Disease Biology, Faculty of Health and Medical Sciences, 
      University of Copenhagen Stigbojlen, Frederiksberg, Denmark.
FAU - Rasmussen, A K I
AU  - Rasmussen AK
AD  - Dako Denmark A/S Produktionsvej, Glostrup, Denmark.
FAU - Fiandaca, M J
AU  - Fiandaca MJ
AD  - AdvanDx Inc. Woburn, MA, USA.
FAU - Kragh, K N
AU  - Kragh KN
AD  - Department for International Health, Immunology and Microbiology, Faculty of 
      Health and Medical Sciences, University of Copenhagen Copenhagen, Denmark.
FAU - Bjarnsholt, T
AU  - Bjarnsholt T
AD  - Department for Clinical Microbiology, University Hospital of Copenhagen Juliane 
      Mariesvej, Copenhagen, Denmark ; Department for International Health, Immunology 
      and Microbiology, Faculty of Health and Medical Sciences, University of 
      Copenhagen Copenhagen, Denmark.
FAU - Hoiby, N
AU  - Hoiby N
AD  - Department for Clinical Microbiology, University Hospital of Copenhagen Juliane 
      Mariesvej, Copenhagen, Denmark ; Department for International Health, Immunology 
      and Microbiology, Faculty of Health and Medical Sciences, University of 
      Copenhagen Copenhagen, Denmark.
FAU - Stender, H
AU  - Stender H
AD  - Stender Diagnostics Gentofte, Denmark.
FAU - Guardabassi, L
AU  - Guardabassi L
AD  - Department of Veterinary Disease Biology, Faculty of Health and Medical Sciences, 
      University of Copenhagen Stigbojlen, Frederiksberg, Denmark.
LA  - eng
PT  - Journal Article
DEP - 20140325
PL  - England
TA  - New Microbes New Infect
JT  - New microbes and new infections
JID - 101624750
PMC - PMC4184662
OTO - NOTNLM
OT  - Cystic fibrosis
OT  - PNA FISH
OT  - Stenotrophomonas maltophilia
OT  - identification
EDAT- 2014/10/31 06:00
MHDA- 2014/10/31 06:01
PMCR- 2014/03/25
CRDT- 2014/10/31 06:00
PHST- 2013/08/22 00:00 [received]
PHST- 2013/12/21 00:00 [revised]
PHST- 2014/01/14 00:00 [accepted]
PHST- 2014/10/31 06:00 [entrez]
PHST- 2014/10/31 06:00 [pubmed]
PHST- 2014/10/31 06:01 [medline]
PHST- 2014/03/25 00:00 [pmc-release]
AID - 10.1002/nmi2.38 [doi]
PST - ppublish
SO  - New Microbes New Infect. 2014 May;2(3):79-81. doi: 10.1002/nmi2.38. Epub 2014 Mar 
      25.