PMID- 25356760
OWN - NLM
STAT- MEDLINE
DCOM- 20150916
LR  - 20181202
IS  - 1537-4505 (Electronic)
IS  - 1531-7129 (Linking)
VI  - 36
IP  - 4
DP  - 2015 Apr
TI  - Local delivery of brain-derived neurotrophic factor on the perforated round 
      window membrane in Guinea pigs: a possible clinical application.
PG  - 705-13
LID - 10.1097/MAO.0000000000000634 [doi]
AB  - HYPOTHESIS/BACKGROUND: Local delivery of neurotrophic factors on the intact round 
      window membrane (RWM) of hair cell-deprived cochleas reduces degeneration of the 
      cochlear nerve. In an animal model of profound hearing loss, we investigated 
      whether this otoprotective effect could be enhanced by perforation of the RWM. 
      Such method could be highly relevant for future clinical applications. METHODS: 
      Guinea pigs were deafened by coadministration of kanamycin and furosemide. Two 
      weeks after deafening, Gelfoam cubes infiltrated with brain-derived neurotrophic 
      factor (BDNF) were deposited onto the RWM of the right cochlea. In the 
      experimental condition, the RWM was perforated. Electrically evoked auditory 
      brainstem responses (eABRs) were recorded weekly. Two or four weeks after Gelfoam 
      placement, both left (untreated) and right (BDNF-treated) cochleas were processed 
      for histology. RESULTS: In BDNF-treated cochleas, both with and without 
      perforation, neural survival in the basal turn of the cochlea was significantly 
      larger than in untreated cochleas. Amplitudes of electrically evoked auditory 
      brainstem responses were larger in BDNF-treated cochleas with an RWM perforation 
      than in those without a perforation and comparable to those of normal-hearing 
      controls. Perforation did not lead to collateral cochlear damage. CONCLUSION: 
      When considering clinical applications of neuroprotective agents such as BDNF, 
      delivery on a perforated RWM seems to be a safe and effective option.
FAU - Havenith, Sarah
AU  - Havenith S
AD  - *Department of Otorhinolaryngology and Head and Neck Surgery and daggerBrain Center 
      Rudolf Magnus, University Medical Center Utrecht, Utrecht, The Netherlands.
FAU - Versnel, Huib
AU  - Versnel H
FAU - Klis, Sjaak F L
AU  - Klis SF
FAU - Grolman, Wilko
AU  - Grolman W
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Otol Neurotol
JT  - Otology & neurotology : official publication of the American Otological Society, 
      American Neurotology Society [and] European Academy of Otology and Neurotology
JID - 100961504
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 59-01-8 (Kanamycin)
RN  - 7LXU5N7ZO5 (Furosemide)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*administration & dosage
MH  - Deafness/chemically induced
MH  - Disease Models, Animal
MH  - *Drug Administration Routes
MH  - Evoked Potentials, Auditory, Brain Stem/*drug effects
MH  - Female
MH  - Furosemide/toxicity
MH  - Guinea Pigs
MH  - Kanamycin/toxicity
MH  - *Round Window, Ear/chemistry
EDAT- 2014/10/31 06:00
MHDA- 2015/09/17 06:00
CRDT- 2014/10/31 06:00
PHST- 2014/10/31 06:00 [entrez]
PHST- 2014/10/31 06:00 [pubmed]
PHST- 2015/09/17 06:00 [medline]
AID - 10.1097/MAO.0000000000000634 [doi]
PST - ppublish
SO  - Otol Neurotol. 2015 Apr;36(4):705-13. doi: 10.1097/MAO.0000000000000634.