PMID- 2535722
OWN - NLM
STAT- MEDLINE
DCOM- 19890118
LR  - 20200724
IS  - 0022-538X (Print)
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Linking)
VI  - 63
IP  - 1
DP  - 1989 Jan
TI  - Isolation of a pathogenic clone of mouse mammary tumor virus.
PG  - 148-58
AB  - Exogenous mouse mammary tumor virus (MMTV) was cloned from a GR mammary tumor. 
      Clone lambda GRT39 contained a full-length integrated MMTV(GR) provirus and both 
      5' and 3' host flanking DNA. The lambda GRT39 provirus had no apparent structural 
      changes associated with cloning and retained the exogenous MMTV gag gene poison 
      sequence. When introduced into rat mammary adenocarcinoma LA7 cells, the lambda 
      GRT39 provirus was fully expressed. lambda GRT39-transfected LA7 cells made MMTV 
      RNA, had gp52 SU protein on the cell surface, and produced B-type retrovirus 
      particles characteristic of MMTV. Mammary tumors developed in hormone-stimulated 
      BALB/c females injected with MMTV from lambda GRT39-transfected LA7 cells [MMTV 
      (lambda GRT39)]. The tumors had new, clonally integrated copies of the 
      MMTV(lambda GRT39) provirus and were expressing MMTV antigen. These data indicate 
      that the lambda GRT39 provirus is biologically active and pathogenic.
FAU - Morris, D W
AU  - Morris DW
AD  - Department of Pathology, School of Medicine, University of California, Davis 
      95616.
FAU - Bradshaw, H D Jr
AU  - Bradshaw HD Jr
FAU - Billy, H T
AU  - Billy HT
FAU - Munn, R J
AU  - Munn RJ
FAU - Cardiff, R D
AU  - Cardiff RD
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Adenocarcinoma/*microbiology
MH  - Animals
MH  - Animals, Newborn
MH  - Blotting, Southern
MH  - Cloning, Molecular
MH  - DNA, Viral/analysis/genetics
MH  - Female
MH  - Gene Expression Regulation
MH  - Mammary Neoplasms, Experimental/*microbiology
MH  - Mammary Tumor Virus, Mouse/genetics/*pathogenicity/ultrastructure
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Microscopy, Electron
MH  - Proviruses/genetics/pathogenicity
MH  - Rats
MH  - Restriction Mapping
MH  - Tumor Cells, Cultured
PMC - PMC247667
EDAT- 1989/01/01 00:00
MHDA- 2001/03/28 10:01
PMCR- 1989/01/01
CRDT- 1989/01/01 00:00
PHST- 1989/01/01 00:00 [pubmed]
PHST- 2001/03/28 10:01 [medline]
PHST- 1989/01/01 00:00 [entrez]
PHST- 1989/01/01 00:00 [pmc-release]
AID - 10.1128/JVI.63.1.148-158.1989 [doi]
PST - ppublish
SO  - J Virol. 1989 Jan;63(1):148-58. doi: 10.1128/JVI.63.1.148-158.1989.