PMID- 25358868 OWN - NLM STAT- MEDLINE DCOM- 20150930 LR - 20240321 IS - 1755-8794 (Electronic) IS - 1755-8794 (Linking) VI - 7 DP - 2014 Oct 31 TI - Sleep quality, BDNF genotype and gene expression in individuals with chronic abdominal pain. PG - 61 LID - 10.1186/s12920-014-0061-1 [doi] LID - 61 AB - BACKGROUND: Sleep quality and genetics may contribute to the etiology of gastrointestinal (GI) symptoms. Individuals with impaired sleep often have a number of associated symptoms including chronic abdominal pain (CAP). The current study examined the interactions of brain-derived neurotrophic factor (BDNF) genotype with sleep quality in persons with CAP and healthy controls. In addition, associations among sleep quality, BDNF genotype, and gene expression were explored in the participants. METHODS: Data were collected on 59 participants (46% male, 61% White, 26.9 +/- 6.6 years; CAP (n=19) and healthy controls (n=40)). Participants with CAP reported poorer sleep quality compared to healthy controls. BDNF genotype, categorized as Val/Val homozygotes versus the Met carriers. RESULTS: Microarray analysis found twenty-four differentially expressed genes by a two-fold magnitude in participants with poor sleep quality compared to good sleep quality, and seven differentially expressed genes comparing CAP to healthy control. Three specific genes in the pain group overlap with sleep quality, including insulin-like growth factor 1 (IGF1), spermatogenesis associated serine-rich 2-like (SPATS2L), and immunoglobulin heavy constant gamma 1 or mu (IGHG1/// IGHM). BDNF was shown to have an interaction effect with GI and sleep symptoms. CONCLUSIONS: Participants with CAP reported poor sleep quality compared to healthy controls. The role of the BDNF Met allele on differential gene expression was not distinct as main factor, but impacted interactions with sleep quality and CAP. Down-regulation of IGF1, SPATS2L, and IGHG1 expression may be related to the etiology of poor sleep quality and CAP. TRIAL REGISTRATION: Clinicaltrial.gov # NCT00824941. FAU - Reddy, Swarnalatha Y AU - Reddy SY FAU - Rasmussen, Nat A AU - Rasmussen NA FAU - Fourie, Nicolaas H AU - Fourie NH FAU - Berger, Rebecca S AU - Berger RS FAU - Martino, Angela C AU - Martino AC FAU - Gill, Jessica AU - Gill J FAU - Longchamps, Ryan AU - Longchamps R FAU - Wang, Xiao Min AU - Wang XM FAU - Heitkemper, Margaret M AU - Heitkemper MM FAU - Henderson, Wendy A AU - Henderson WA LA - eng SI - ClinicalTrials.gov/NCT00824941 GR - ZIA NR000018/Intramural NIH HHS/United States GR - 1ZIANR000018-01-04/PHS HHS/United States PT - Comparative Study PT - Journal Article PT - Research Support, N.I.H., Extramural PT - Research Support, N.I.H., Intramural DEP - 20141031 PL - England TA - BMC Med Genomics JT - BMC medical genomics JID - 101319628 RN - 0 (Biomarkers) RN - 0 (Brain-Derived Neurotrophic Factor) RN - 0 (Carrier Proteins) RN - 0 (IGF1 protein, human) RN - 0 (Proteins) RN - 0 (RNA, Messenger) RN - 0 (SPATS2L protein, human) RN - 0 (prolactin-binding protein) RN - 67763-96-6 (Insulin-Like Growth Factor I) RN - 7171WSG8A2 (BDNF protein, human) SB - IM MH - Abdominal Pain/*genetics/*physiopathology MH - Adult MH - Biomarkers/*metabolism MH - Brain-Derived Neurotrophic Factor/*genetics MH - Carrier Proteins/genetics MH - Case-Control Studies MH - Chronic Disease MH - Female MH - *Gene Expression Profiling MH - Genotype MH - Humans MH - Insulin-Like Growth Factor I/genetics MH - Male MH - Oligonucleotide Array Sequence Analysis MH - Polymorphism, Genetic/*genetics MH - Proteins/genetics MH - RNA, Messenger/genetics MH - Real-Time Polymerase Chain Reaction MH - Reverse Transcriptase Polymerase Chain Reaction MH - Sleep Initiation and Maintenance Disorders/*genetics PMC - PMC4226913 EDAT- 2014/11/02 06:00 MHDA- 2015/10/01 06:00 PMCR- 2014/10/31 CRDT- 2014/11/01 06:00 PHST- 2013/08/08 00:00 [received] PHST- 2014/10/09 00:00 [accepted] PHST- 2014/11/01 06:00 [entrez] PHST- 2014/11/02 06:00 [pubmed] PHST- 2015/10/01 06:00 [medline] PHST- 2014/10/31 00:00 [pmc-release] AID - s12920-014-0061-1 [pii] AID - 61 [pii] AID - 10.1186/s12920-014-0061-1 [doi] PST - epublish SO - BMC Med Genomics. 2014 Oct 31;7:61. doi: 10.1186/s12920-014-0061-1.