PMID- 25359601
OWN - NLM
STAT- MEDLINE
DCOM- 20150410
LR  - 20181113
IS  - 1432-2307 (Electronic)
IS  - 0945-6317 (Linking)
VI  - 466
IP  - 1
DP  - 2015 Jan
TI  - Hyalinizing clear cell carcinoma with EWSR1-ATF1 fusion gene: report of three 
      cases with molecular analyses.
PG  - 37-43
LID - 10.1007/s00428-014-1676-5 [doi]
AB  - Hyalinizing clear cell carcinoma (HCCC) is a low-grade salivary gland carcinoma 
      characterized by clear cells and hyalinized stroma. Recently, the EWSR1-ATF1 
      fusion gene was found in HCCCs. We herein describe three cases of HCCC identified 
      in one male and two females, ranging in age from 27 to 67 years. The tumors were 
      located in the root of tongue, nasopharynx, and soft palate. They were composed 
      of nested or cord-like proliferations of epithelial cells with clear to pale 
      eosinophilic cytoplasm, embedded in hyalinized and focally fibroedematous stroma. 
      Tumor-associated lymphoid proliferation and pseudoepitheliomatous hyperplasia 
      were also observed in each one case. MAML2 fusions specific to mucoepidermoid 
      carcinoma were not detected in any of the three cases. We found EWSR1-ATF1 in two 
      of three HCCCs using reverse transcription polymerase chain reaction (RT-PCR) 
      with our original primer sets designed to detect the fusion gene transcripts in 
      formalin-fixed paraffin-embedded (FFPE) tissues. EWSR1 rearrangement was also 
      confirmed by fluorescence in situ hybridization (FISH) on FFPE sections in two 
      cases. There was a good concordance between the two methods (two positive cases 
      and one negative case by both RT-PCR and FISH). Therefore, RT-PCR and FISH using 
      FFPE tissue may be ancillary tools to confirm the diagnosis of HCCC.
FAU - Nakano, Takafumi
AU  - Nakano T
AD  - Department of Anatomic Pathology, Graduate School of Medical Sciences, Kyushu 
      University, 3-1-1 Maidashi, Higashi-ku, Fukuoka, 812-8582, Japan.
FAU - Yamamoto, Hidetaka
AU  - Yamamoto H
FAU - Nishijima, Toshimitsu
AU  - Nishijima T
FAU - Tamiya, Sadafumi
AU  - Tamiya S
FAU - Shiratsuchi, Hideki
AU  - Shiratsuchi H
FAU - Nakashima, Torahiko
AU  - Nakashima T
FAU - Komune, Shizuo
AU  - Komune S
FAU - Oda, Yoshinao
AU  - Oda Y
LA  - eng
PT  - Case Reports
PT  - Journal Article
DEP - 20141031
PL  - Germany
TA  - Virchows Arch
JT  - Virchows Archiv : an international journal of pathology
JID - 9423843
RN  - 0 (ATF1 protein, human)
RN  - 0 (Activating Transcription Factor 1)
RN  - 0 (Calmodulin-Binding Proteins)
RN  - 0 (EWSR1 protein, human)
RN  - 0 (RNA-Binding Protein EWS)
RN  - 0 (RNA-Binding Proteins)
SB  - IM
MH  - Activating Transcription Factor 1/*genetics
MH  - Adenocarcinoma, Clear Cell/diagnosis/*genetics/metabolism
MH  - Adult
MH  - Aged
MH  - Calmodulin-Binding Proteins/*genetics
MH  - Female
MH  - Gene Fusion/*genetics
MH  - Gene Rearrangement/genetics
MH  - Humans
MH  - Hyalin/*metabolism
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Middle Aged
MH  - Nasopharynx
MH  - Palate, Soft
MH  - Polymerase Chain Reaction
MH  - RNA-Binding Protein EWS
MH  - RNA-Binding Proteins/*genetics
MH  - Salivary Gland Neoplasms/diagnosis/*genetics/metabolism
MH  - Tongue
EDAT- 2014/11/02 06:00
MHDA- 2015/04/11 06:00
CRDT- 2014/11/01 06:00
PHST- 2014/06/19 00:00 [received]
PHST- 2014/10/20 00:00 [accepted]
PHST- 2014/10/02 00:00 [revised]
PHST- 2014/11/01 06:00 [entrez]
PHST- 2014/11/02 06:00 [pubmed]
PHST- 2015/04/11 06:00 [medline]
AID - 10.1007/s00428-014-1676-5 [doi]
PST - ppublish
SO  - Virchows Arch. 2015 Jan;466(1):37-43. doi: 10.1007/s00428-014-1676-5. Epub 2014 
      Oct 31.