PMID- 25359683
OWN - NLM
STAT- MEDLINE
DCOM- 20150917
LR  - 20190221
IS  - 1097-4644 (Electronic)
IS  - 0730-2312 (Linking)
VI  - 116
IP  - 3
DP  - 2015 Mar
TI  - MicroRNA profile of tumorigenic cells during carcinogenesis of lung 
      adenocarcinoma.
PG  - 458-66
LID - 10.1002/jcb.24999 [doi]
AB  - To obtain microRNA (miRNA) profile and clarify their biological function in 
      tumorigenic Sca-1(+) CD34(+) cells during carcinogenesis of lung adenocarcinoma. 
      After intranasal infection with recombinant Adeno-Cre viruses (AdV-Cre), lung 
      adenocarcinoma was identified pathologically in Lox-stop-lox Kras (LSL-Kras) G12D 
      mice. Sca-1(+) CD34(+) cells were sorted by flow cytometry and tested for 
      tumor-initiating ability, self-renewal and tumorigenicity. MiRNA profiles were 
      obtained using microarray and further confirmed by real-time RT-PCR (qRT-PCR). 
      MiRNA functions were predicted bioinformatically, and miR-294 function was 
      verified to explore its role in tumor migration and invasion. Lung adenocarcinoma 
      was induced in LSL-Kras G12D mice within 30 days. In vivo, the tumorigenicity of 
      Sca-1(+) CD34(+) cells was 25 times stronger than Sca-1(-) CD34(-) cells. During 
      tumorigenesis of lung adenocarcinoma, the expression of 145 miRNAs in Sca-1(+) 
      CD34(+) cells increased and 72 miRNAs decreased (P < 0.01). Four successively 
      up-regulated miRNAs (miR-15a*, miR-203, miR-294 and miR-295*) and three 
      successively down-regulated ones (miR-19b, miR-483 and miR-615-5p) were 
      identified. Among them, miR-294 could constitutively bind to 3'-UTR of matrix 
      metalloproteinase 3 (MMP3), and down-regulate MMP3 protein expression. MiR-294 
      also significantly inhibited migration and invasion of Lewis lung cancer cells. 
      MiRNAs are characteristically expressed in tumor-initiating Sca-1(+) CD34(+) 
      cells of lung adenocarcinoma, and may play important roles during the 
      carcinogenesis of lung adenocarcinoma.
CI  - (c) 2014 Wiley Periodicals, Inc.
FAU - Zhao, Zhen-guo
AU  - Zhao ZG
AD  - Cancer Institute of PLA, Xinqiao Hospital, Third Military Medical University, 
      Chongqing, PR China.
FAU - Jin, Jun-yu
AU  - Jin JY
FAU - Zhang, An-mei
AU  - Zhang AM
FAU - Zhang, Lu-ping
AU  - Zhang LP
FAU - Wang, Xin-xin
AU  - Wang XX
FAU - Sun, Jian-guo
AU  - Sun JG
FAU - Chen, Zheng-tang
AU  - Chen ZT
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Cell Biochem
JT  - Journal of cellular biochemistry
JID - 8205768
RN  - 0 (Antigens, CD34)
RN  - 0 (Antigens, Ly)
RN  - 0 (Ly6a protein, mouse)
RN  - 0 (Membrane Proteins)
RN  - 0 (MicroRNAs)
SB  - IM
ECI - J Cell Biochem. 2015 Sep;116(9):2120. PMID: 26205483
MH  - Adenocarcinoma/*genetics/pathology
MH  - Adenocarcinoma of Lung
MH  - Animals
MH  - Antigens, CD34/metabolism
MH  - Antigens, Ly/metabolism
MH  - Carcinogenesis/*genetics/pathology
MH  - Carcinoma, Lewis Lung/genetics/pathology
MH  - Cell Proliferation
MH  - Cell Separation
MH  - Disease Models, Animal
MH  - *Gene Expression Profiling
MH  - Gene Expression Regulation, Neoplastic
MH  - HEK293 Cells
MH  - Humans
MH  - Lung Neoplasms/*genetics/pathology
MH  - Membrane Proteins/metabolism
MH  - Mice, Inbred BALB C
MH  - Mice, Nude
MH  - MicroRNAs/genetics/*metabolism
MH  - Neoplastic Stem Cells/metabolism/pathology
MH  - Reproducibility of Results
OTO - NOTNLM
OT  - CARCINOGENESIS
OT  - LUNG ADENOCARCINOMA
OT  - TUMORIGENIC CELLS
OT  - miRNAs
EDAT- 2014/11/02 06:00
MHDA- 2015/09/18 06:00
CRDT- 2014/11/01 06:00
PHST- 2014/05/10 00:00 [received]
PHST- 2014/10/20 00:00 [accepted]
PHST- 2014/11/01 06:00 [entrez]
PHST- 2014/11/02 06:00 [pubmed]
PHST- 2015/09/18 06:00 [medline]
AID - 10.1002/jcb.24999 [doi]
PST - ppublish
SO  - J Cell Biochem. 2015 Mar;116(3):458-66. doi: 10.1002/jcb.24999.