PMID- 25359716
OWN - NLM
STAT- MEDLINE
DCOM- 20170626
LR  - 20170626
IS  - 1600-0501 (Electronic)
IS  - 0905-7161 (Linking)
VI  - 27
IP  - 2
DP  - 2016 Feb
TI  - Strontium content and collagen-I coating of Magnesium-Zirconia-Strontium implants 
      influence osteogenesis and bone resorption.
PG  - e15-24
LID - 10.1111/clr.12511 [doi]
AB  - OBJECTIVE: Our objective was to study the role of Collagen type-I (Col-I) coating 
      on Magnesium-Zirconia (Mg-Zr) alloys, containing different quantities of 
      Strontium (Sr), in enhancing the in vitro bioactivity and in vivo bone-forming 
      and mineralisation properties of the implants. MATERIALS AND METHODS: MC3T3-E1 
      osteoblast cell line was used to analyse the in vitro properties of Col-I coated 
      and uncoated alloys. Cell viability analysis was performed by MTT assay; cell 
      attachment on alloy surfaces was studied by scanning electron microscopy (SEM); 
      and gene profiling of bone-specific markers in cells plated on uncoated alloys 
      was performed by Quantitative RT-PCR. In vivo studies were performed by 
      implanting 2-mm-sized cylindrical pins of uncoated and coated alloys in male New 
      Zealand white rabbits (n = 33). Bone formation and mineralisation was studied by 
      Dual Energy X-ray Absorptiometry (DXA) and histological analysis at one and three 
      months post-implantation. RESULTS: Our results clearly showed that Sr content and 
      Col-I coating of Mg-Zr-Sr alloys significantly improved their bone inducing 
      activity in vitro and in vivo. Osteoblasts on coated alloys showed better 
      viability and surface binding than those on uncoated alloys. Sr inclusion in the 
      alloys enhanced their bone-specific gene expression. The in vivo activity of 
      implants with higher Sr and Col-I coating was superior to uncoated and other 
      coated alloys as they showed faster bone induction and higher mineral content in 
      the newly formed bone. CONCLUSION: Our results indicate that bone-forming and 
      mineralising activity of Mg-Zr-Sr implants can be significantly improved by 
      controlling their Sr content and coating their surface with Col-I.
CI  - (c) 2014 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.
FAU - Mushahary, Dolly
AU  - Mushahary D
AD  - Institute for Frontier Materials, Deakin University, Geelong, Vic, Australia.
FAU - Wen, Cuie
AU  - Wen C
AD  - Faculty of Engineering and Industrial Sciences, Swinburne University of 
      Technology, Hawthorn, Vic, Australia.
FAU - Kumar, Jerald M
AU  - Kumar JM
AD  - CSIR-Centre for Cellular and Molecular Biology, Hyderabad, India.
FAU - Sravanthi, Ragamouni
AU  - Sravanthi R
AD  - CSIR-Centre for Cellular and Molecular Biology, Hyderabad, India.
FAU - Hodgson, Peter
AU  - Hodgson P
AD  - Institute for Frontier Materials, Deakin University, Geelong, Vic, Australia.
FAU - Pande, Gopal
AU  - Pande G
AD  - CSIR-Centre for Cellular and Molecular Biology, Hyderabad, India.
FAU - Li, Yuncang
AU  - Li Y
AD  - Institute for Frontier Materials, Deakin University, Geelong, Vic, Australia.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20141031
PL  - Denmark
TA  - Clin Oral Implants Res
JT  - Clinical oral implants research
JID - 9105713
RN  - 0 (Coated Materials, Biocompatible)
RN  - 0 (Collagen Type I)
RN  - C6V6S92N3C (Zirconium)
RN  - I38ZP9992A (Magnesium)
RN  - S38N85C5G0 (zirconium oxide)
RN  - YZS2RPE8LE (Strontium)
MH  - Absorptiometry, Photon
MH  - Animals
MH  - Bone Resorption/*chemically induced
MH  - Cell Line
MH  - Cell Survival
MH  - Coated Materials, Biocompatible/*pharmacology
MH  - Collagen Type I/*pharmacology
MH  - Magnesium/*pharmacology
MH  - Microscopy, Electron, Scanning
MH  - Osteoblasts
MH  - Osteogenesis/*drug effects
MH  - Rabbits
MH  - Real-Time Polymerase Chain Reaction
MH  - Strontium/*pharmacology
MH  - Zirconium/*pharmacology
OTO - NOTNLM
OT  - bone remodelling
OT  - calcification
OT  - gene expression
OT  - matrix mineralisation
OT  - osteoclasts
EDAT- 2014/11/02 06:00
MHDA- 2017/06/27 06:00
CRDT- 2014/11/01 06:00
PHST- 2014/09/16 00:00 [accepted]
PHST- 2014/11/01 06:00 [entrez]
PHST- 2014/11/02 06:00 [pubmed]
PHST- 2017/06/27 06:00 [medline]
AID - 10.1111/clr.12511 [doi]
PST - ppublish
SO  - Clin Oral Implants Res. 2016 Feb;27(2):e15-24. doi: 10.1111/clr.12511. Epub 2014 
      Oct 31.